Collagen is the most widely used extracellular matrix (ECM) protein for cell culture, facilitating cell attachment, growth, differentiation, migration, and tissue morphogenesis.

We offer Collagen I Rat Tail (in liquid form and on precoated tissue culture plates) and Collagen I Bovine (in liquid form) for 3D applications and as coating solution.

Collagen I Rat Tail and Collagen I Bovine, Liquid Form

We offer Collagen I Rat Tail and Collagen I Bovine at 5 mg/mL concentration which gives the flexibility to dilute to lower concentrations.

Figure shows Gibco™ Collagen I and collagen from two other suppliers were prepared at the concentrations stated and allowed to solidify in individual wells of a 24-well plate. Gibco Collagen I forms a firm, clearer gel matrix for cell culture.

Collagen I, Coated Plates

  • Ready to use right from the package
  • Available in 6-well, 24-well, and 96-well formats
  • High-quality, consistent coating of rat tail collagen I  for culturing primary cells including hepatocytes and keratinocytes
  • Convenient room temperature storage

Key Applications for Collagen I

Collagen consists of three α-chains which form a triple helix, providing tensile strength to the extracelluar matrix (ECM). Collagen I is the most common fibrillar collagen and is found in skin, bone, tendons, and other connective tissues.

Collagen I plays key roles in cell biology and biomedical applications: 

  • Promotes attachment, proliferation, and differentiation in breast cancer cells1
  • Forms gland-like circular structures from embryonic stem cells2
  • Allows better representation of endometrial physiology and morphology in vitro, allowing for in vivo studies of endometrial cancer cell invasion3
  • Helps microvascular endothelial cells (HMVEC cells) adopt an in vitro spindle-shaped morphology and form solid cord-like assemblies4
  • Used for in vitro angiogenesis assays, is a barrier in cell invasion assays, and enables cell adhesion studies5-6
  • Supports a variety of cell types, including primary colon carcinoma, mouse liver progenitor, and rat pancreatic islet cells


1.  Chandrasekaran, S., et al., J. Biol. Chem. 274(16):11408 (1999).
2.  Chen, S., R. et al. (2003) Stem Cells 21: 281-295.
3.  Park, D., et al. (2003) Cancer Letters 195: 185-192.
4.  Whelan. M.C., et al. (2003) J. Biol. Chem. Jan 3;278 (1):327-34
5.  Kokenyesi, R., K., et al. (2003) Gynecol Oncol 89: 60-72.
6.  Ritty,T. et al. (2003) J Ortho Res 21: 442-450