Learn about cell culture and transfection methods and technologies

Cell culture and transfection are foundational techniques for life scientists, translational researchers, drug discovery and pre-clinical testing facilities, bioproduction, biobanking, and biorepository centers, and many other academic and applied efforts. Understanding (and effectively utilizing) the various cell lines, media and supplement systems, transfection techniques, incubators and biological safety cabinets, cultureware, and cryopreservation techniques can mean the difference between success and failure.

The purpose of this learning center is to connect scientists (whether new or experienced) to our many resources for learning about cell culture and transfection methods and techniques to help ensure your success every time you step into the lab.

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Topics in cell culture & transfection

Cell culture & transfection features

Virtual lab training

The Gibco Education virtual labs represent a unique interactive cell culture training experience. Learn everything from the fundamentals of cell culture to key techniques for primary and stem cell cultures and successful protein expression. As you progress through each lab, you can certify your knowledge by taking in-lab quizzes.


Intended as an introduction to cell culture, the handbook covers topics such as getting familiar with the requirements of a laboratory dedicated to cell culture experiments, laboratory safety, aseptic technique, and microbial contamination of cell cultures, as well as providing basic methods for passaging, freezing, and thawing cultured cells.


This on-demand webinar provides important points that will help you work with your biological safety cabinet more effectively to prevent contamination in your cell culture lab. (Registration required)

Cell culture & transfection subtopics

Cell culture information

Cell culture is viewed as one of those enigmatic techniques by some researchers. There are so many parameters to consider: which cells to use, what media and supplements are appropriate, how often to feed, how to deal with contamination, etc. We've put together a collection of resources that we hope will give you the confidence to get started and to continuously improve your skills in the cell culture room.

Transfection information

Whether for protein expression, gene expression modulation, or genome editing, efficient, effective transfection relies on an optimal match between the cultured cells, the transfection reagent or equipment, and post-transfection care and maintenance. Get the information you need to get the results you're aiming for.

Cryopreservation information

Preparation is key. Improper cryogenic preservation can have disastrous outcomes, leading to poor (or no!) cell recovery and project setbacks. Review information on cryopreservation and cold storage best practices before you freeze your first vial.

Cell culture equipment information

Biosafety cabinets, CO2 incubators, centrifuges, orbital shakers, media filters and lab cultureware...there's a lot to understand to keep your cultures healthy, thriving, and contamination-free. Use our collection of resources on cell culture equipment to boost your effectiveness with all of the tasks of cell culture.

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Cell Culture & Transfection Resource Library

Browse cell culture & transfection learning center content by collection (resource type)—application notes, case studies, videos, webinars, and white papers.

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