3D cell culture and analysis and the study of organoids and spheroids are becoming more prevalent as a research method in publications. Traditional 2D cell cultures lack the organizational complexity and longevity needed to serve as effective models. 3D cell culture can offer more physiologically relevant testing models, and, as experimental techniques are refined and methods are optimized, the use of 3D cell culture, organoids, and spheroids in applications such as disease modeling and regenerative medicine are becoming more common.

The purpose of this learning center is to connect researchers to our many resources for information about culture and analysis of 3D models and to highlight effective protocols, methods, and techniques to help ensure your success.

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FREE 3D eLearning Courses

We are now offering an ever-expanding set of free e-Learns to pass on the expertise and assist with advancing your research. Follow the links below and start your free learning adventure in the basics of 3D and then progress to our neural organoid training course.

Getting started in 3D cell culture
Learn more at: thermofisher.com/3dbasics-elearn

Neural organoid generation from PSCs
Learn more at: thermofisher.com/neuralorganoid-elearn


Researchers utilize cell lines often to elucidate disease models of interest. Gibco cell lines allow you to closely mimic the in vivo state and generate more physiologically relevant data for organoid and spheroid research.

  • Primary cells education—Learn more about our primary cell culture tools, protocols, FAQs and custom services
  • Neuronal cells—Explore our Neuro protocols area and learn more about neural cell culture and differentiation, cell analysis, molecular characterization, and transfection
  • Stem cells—Harness the power of the stem cell and find out more about our extensive educational resources supporting stem cell research

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Thermo Scientific Nunclon Sphera culture systems are designed for spheroid culture (e.g., 3D tumor spheroids) and organoid culture (e.g., brain organoids) with a wide selection of format from plates to dishes and flasks. 

  • Application note—Harnessing new dimensions in your research: coming ‘round to cancer spheroid culture
  • Article—Formation of uniform and reproducible 3D cancer spheroids in high-throughput plates
  • Blog post—A brief history of spheroids and 3D culture


Gibco media

Cited in numerous organoid and spheroid peer-reviewed papers, Gibco media and reagents are widely used in the growth, differentiation and maturation of cellular 3D models. This is where a culture system can be developed to suit specialized cell types like stem cells or cancer cell lines.

  • Cell culture and supplements—Explore our many resources for learning about cell culture methods and techniques
  • Growth factors—Explore our “Growth Facts” section where we have compiled a list from experienced researchers of important facts about growth factors