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Recombinant proteins information

Many advanced cell culture models rely on bioactive recombinant proteins to achieve the desired phenotype(s). Learn what recombinant proteins are, how they can be used, and how to handle them so you’re set up for success.

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Cell Culture & Transfection Application Notes

Search our application notes to find research applications and proof-of-concept experiments involving various products from our cell biology portfolio.

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Blog:  Advancing Cell Culture

Find cell culture news, best practices and informative resources for everything from cell isolation and growth to cell analysis, harvesting and storage.

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Application note3D spheroid culture as a tool for studying drug metabolism 3D cell culture
Application note5 Reasons to enhance your cell culture using variable oxygen control CO2 incubation
Application noteApplication properties of materials used for porous membranes in cell culture inserts Cultureware
Application noteAspects of cryo preservation Cryopreservation
Application noteBack to basics: Proper care and maintenance for your cell culture incubator CO2 incubation
Application noteBiological safety cabinet filter testing—Lessons from overseas Biological safety cabinets
Application noteCell culture contamination: Evaluating contamination prevention solutions for the CO2 incubator CO2 incubation
Application noteCell culture contamination: The role of the CO2 incubator in reducing contamination CO2 incubation
Application noteCell culture contamination: Understanding the causes and managing the risks General cell culture
Application noteCell harvesting by temperature reduction—Nunc UpCell Surface Cultureware
Application noteChoosing the right orbital shaker for your application Orbital shakers
Application noteCompatibility of mounting media on Nunc Permanox Slides Cultureware
Application noteCryopreservation of mammalian cells Cryopreservation
Application noteCulturing cells under hypoxic conditions for biologically relevant results Filtration
Application noteCulturing embryonic stem cells using media filtered with Thermo Scientific Nalgene Rapid-Flow PES filter units Filtration
Application noteGeneration of MCF7 spheroids in serum-free conditions 3D cell culture
Application noteDecontamination cycles in BBD 6220 and Heracell incubators completely eliminate MycoplasmaCO2 incubation
Application noteDelivery solutions for immune cellsTransfection
Application noteDifferentiation of pluripotent stem cells into neural organoids 3D cell culture
Application noteDoes a larger fluid reservoir give me more cooling capacity and improved temperature stability?  ENESDEBaths, circulators, chillers
Application noteEffective heat sterilization in CO2 incubators CO2 incubation
Application noteEliminate incubator contamination with Thermo Scientific Heracell incubators CO2 incubation
Application noteEnhanced expansion of neural stem cells using a heat stable bFGF General cell culture
Application noteEnhanced proliferation of primary NSCs and sustained differentiation into precursors using heat-stable bFGF General cell culture
Application noteErgonomics and safety in biological research cabinets  Biological safety cabinets
Application noteFeeder cell and extracellular matrix-free cultivation of hPSC on Nunclon Vita with Rho-kinase inhibitor Cultureware
Application noteFlow rate and throughput of cell culture media and serum using Nalgene Rapid-Flow filters with 0.2 micron PES membrane Cultureware
Application noteGeneration and transfection of induced pluripotent stem cells (iPSCs)Transfection
Application noteHow can a water-cooled condenser option significantly reduce energy consumption and heat output without compromising sample protection? Cold storage
Application noteHow can blood banks eliminate potential variables to ensure consistent product yield and reproducibility in centrifugation? Centrifugation
Application noteHow can blood banking and bioprocessing production facilities enhance ergonomics, while simplifying operation for greater productivity during their separations? Centrifugation
Application noteHow can I help improve safety in my laboratory when spinning pathogens or patient samples in swing-out rotors? Centrifugation
Application noteHow can instant rotor identification enhance productivity and safety during your centrifuge set-up and run? Centrifugation
Application noteHow can using the wrong type of water to provide in-chamber humidity cause corrosion in my CO2 incubator? CO2 incubation
Application noteHow can you extend the filter life of your BSC? Biological safety cabinets
Application noteHow do carbon fiber rotors ensure greater sample integrity than metal rotors during the sample preparation process? Centrifugation
Application noteHow do I ensure that my heated bath circulator will be able to control to that temperature rate? Baths, circulators, chillers
Application noteHow do I select an energy efficient refrigerated bath circulator? Baths, circulators, chillers
Application noteHow does a CO2 resistant shaker provide greater reliability, flexibility and ease of use for varied cell culture applications, compared to a dedicated incubated CO2 shaker? Orbital shakers
Application noteHow does a low-binding culture surface compare to a non-treated surface in supporting cancer spheroid cultures? Cultureware
Application noteHow does Thermo Scientific Auto-Lock rotor exchange facilitate rotor placement and improve safety, compared with traditional rotor tie-down systems?  EN DECentrifugation
Application noteHow is it possible to reduce energy consumption and environmental impact with production-level centrifuges? Centrifugation
Application noteHow will the cost of purchasing and running a recirculating chiller or bath circulator affect my bottom line? Baths, circulators, chillers
Application noteI need an ultra-low freezer to store my samples. Does the initial pull down time of a freezer indicate how well the unit can protect my samples after a door opening? Cold storage
Application noteInduction of cancer cell migration using a heat stable bFGF General cell culture
Application noteIn vitro evaluation of hepatic function using a primary human hepatocyte 3D spheroid culture system3D cell culture
Application noteIn vitro evaluation of hepatic gene expression using a Gibco 3D spheroid culture system 3D cell culture
Application noteI plan on using my bath circulator for external circulation only. Should I get a large reservoir that can absorb the heat for more stability?  EN ES DEBaths, circulators, chillers
Application noteI want to keep my samples at a temperature of 37°C. Which type of water bath is ideal for this requirement: general purpose, circulating or shaking? Baths, circulators, chillers
Application noteI want to use a bath circulator to immerse my vessels. How do I select the right size bath for my application?  EN ES DEBaths, circulators, chillers
Application noteI’m hearing a lot about SNAP in my lab. What is SNAP? Cold storage
Application noteImportance of Class 100 air in a CO2 incubator CO2 incubation
Application noteImprove genome editing outcomes in biologically relevant cell modelsTransfection
Application noteImprove lentiviral production using Lipofectamine 3000 reagentTransfection
Application noteiPSC generation using Lipofectamine transfection reagent Transfection
Application noteIs my -80°C freezer designed to prepare samples for long-term storage? Cold storage
Application noteIs the use of hydrocarbon refrigerants in laboratory refrigerators and freezers safe? Cold storage
Application noteIs there a safer way to store potentially flammable, pharmaceutical-grade materials that require refrigerated or frozen temperatures? Cold storage
Application noteIsolation of mitochondria using the Thermo Scientific Sorvall LYNX 6000 superspeed centrifuge Centrifugation
Application noteI’ve heard that switching to −70°C for ultra-low freezers can reduce energy, but is that safe for my sample? Cold storage
Application noteLarge-scale recombinant protein production using the Thermo Scientific Sorvall LYNX 6000 Superspeed Centrifuge and Fiberlite Large Capacity Rotors Centrifugation
Application noteLarge volume precipitation of proteins with ammonium sulfate using Thermo Scientific Fiberlite Carbon Fiber Rotors Centrifugation
Application noteNeurobiology transfection guideTransfection
Application noteOptimizing neuron adhesion and growth by choosing the right surface of Thermo Scientific Chamber Slide General cell culture
Application noteOxygen: Too much of a good thing? CO2 incubation
Application notePelleting efficiency of patented tilted adapters using the new Thermo Scientific benchtop 1L centrifuges: a comparison of pelleting in Thermo Scientific benchtop and superspeed floor-model centrifuges Centrifugation
Application notepH and pressure issues in closed cell culture vessels Cultureware
Application noteProper care and maintenance of your cell culture incubator CO2 incubation
Application noteReduced evaporation in Nunc Edge plate with solid reservoir Cultureware
Application noteSeparation of PBMCs from blood samples using the new Thermo Scientific benchtop 1L centrifuge Centrifugation
Application noteStem cell transfection guideTransfection
Application noteSuperior imaging quality of Nunc Optical Bottom Plate Cultureware
Application noteThe Thermo Scientific Carrier Plate enables precise adjustment of height for Cell Culture Inserts in multiwell dishes Cultureware
Application noteThe Thermo Scientific Nunclon Sphera surface supports effective formation of three dimensional cancer spheroids in suspension Cultureware
Application noteThermo Scientific Biosafety: A European view Biological safety cabinets
Application noteThermo Scientific Nunc TripleFlask culturing technique Cultureware
Application noteTotal thermal disinfection of CO2 incubators CO2 incubation
Application noteUneven cell growth during incubation Cultureware
Application noteUniversity of Michigan field study of Class II Biological Safety Cabinet energy consumption costs Biological safety cabinets
Application noteWhat steps can I take to ensure that my heated bath circulator can actually bring my application to setpoint temperature? Baths, circulators, chillers
Application noteWhat do I need to consider when preparing samples in bags using a controlled-rate freezer? Cold storage
Application noteWhat kind of storage tube should I use to prevent the breakdown of RNA in tissue during cold storage? Cold storage
Application noteWhat technologies make it possible to reduce energy consumption and environmental impact with high performance floor model centrifuges? Centrifugation
Application noteWhy should you select a Class II Biological Safety Cabinet (BSC) with a DC motor over one with an AC motor? Biological safety cabinets
Application noteWhere is the highest risk of contamination in your biological safety cabinet (BSC): the HEPA filters or the airflow balance at the front opening? Biological safety cabinets
Application noteWhich incubation parameters are most important for proper cell growth and expression? CO2 incubation
Application noteWhy are airflow alarms an important consideration for biological safety cabinets (BSCs) Biological safety cabinets
Application noteWhy does the location of sensors in my CO2 incubator affect responses from my cultured cells? CO2 incubation
Application noteWhy is a circulating fan essential to superior growth conditions in a CO2 cell culture incubator? CO2 incubation
Application noteWhy is a circulating fan essential to superior growth conditions in a cell culture incubator? CO2 incubation
Application noteWhy is a dual blower system better than a single blower system? Biological safety cabinets
Application noteWhy is automated heat decontamination a better solution for sterilizing your CO2 incubator than an automated chemical disinfection? CO2 incubation
BlogA Brief History of Spheroids3D cell culture
BlogAdvancing Cell CultureGeneral cell culture
BlogFormation of Uniform and Reproducible 3D Cancer Spheroids in High-Throughput Plates3D cell culture
BlogThe use of spheroids in cancer research3D cell culture
CitationCytotoxicity testing of burn wound dressings, ointments and creams: a method using polycarbonate cell culture inserts on a cell culture system Cultureware
CitationOptimizing parameters for clinical-scale production of high IL-12 secreting dendritic cells pulsed with oxidized whole tumor cell lysate Cultureware
CitationPro-apoptotic molecule suppression using siRNA delivery Transfection
HandbookA guide for proper cryogenic preservation Cryopreservation
HandbookGibco Cancer Basics Handbook General cell culture
HandbookGibco Cell Culture Basics HandbookGeneral cell culture
HandbookOne Cell Bio Product Handbook General cell culture
HandbookPluripotent Stem Cell HandbookGeneral cell culture
HandbookPrimary Cell Culture Sourcebook General cell culture
HandbookProtein Expression HandbookGeneral cell culture
HandbookSkin and Eye Primary Cell Sourcebook General cell culture
Mobile appGibco Cell Culture Companion General cell culture
ProtocolSKOV-3 Cell Line Spheroid Generation for HT Assays3D cell culture
ProtocolT47D Cell Line Spheroid Generation for HT Assays3D cell culture
ProtocolMDA-MB-231 Cell Line Spheroid Generation for HT3D cell culture
ProtocolHepG2 Cell Line Spheroid Generation for HT Assays3D cell culture
ProtocolLNCaP Cell Line Spheroid Generation for HT Assays3D cell culture
ProtocolPC-3 Cell Line Spheroid Generation for HT Assays3D cell culture
Protocol3D cell culture protocols3D cell culture
ProtocolCell culture protocolsGeneral cell culture
ProtocolNeural cell culture protocolsGeneral cell culture
ProtocolPrimary cell protocolsGeneral cell culture
ProtocolSerum-free media protocolsGeneral cell culture
ProtocolStem cell protocolsGeneral cell culture
ProtocolTransfecting neural cells using Neon Transfection SystemTransfection
ProtocolTransfection protocolsTransfection
ProtocolTransfection protocols (by cell type)Transfection
ProtocolTransfection protocols (by reagent)Transfection
Scientific poster3D Scientific Presentations (Posters and App Notes) 3D cell culture
Scientific posterAnalysis of cellular processes within 3D cell models using fluorescence microscopy 3D cell culture
Scientific posterCo-culture of Primary Hepatic Stellate Cells and Hepatocytes in Modeling of Liver Fibrosis in 2D and 3D Spheroids 3D cell culture
Scientific posterEvaluating antibody-mediated cellular cytotoxicity and potency of antibody-drug conjugates within three-dimensional tumor models (SITC 2018) 3D cell culture
Scientific posterGibco BenchStable Media Poster: Novel Basal Cell Culture Media Formulations Enable Long-Term Storage at Room Temperature General cell culture
Scientific posterGibco Heat Stable bFGF Poster: Improved Expansion of Neural Stem Cells with Gibco Heat Stable Recombinant Human General cell culture
Scientific posterGibco Heat Stable bFGF Poster: Novel Engineered Basic Fibroblast Growth Factor Improves Stability and Enables Improved Cell Culture Outcomes General cell culture
Scientific posterIn vitro evaluation of primary animal hepatic function using a 3D-spheroid culture system 3D cell culture
Scientific posterMatching refractive index of mounting media improves axial resolution and image quality in 3D biological samples 3D cell culture
Scientific posterNew and improved cellular health evaluation of 2D and 3D cellular models using microplate reader assays (SLAS 2019) 3D cell culture
Scientific posterOptimization of HepaRG workflow for use in 3D spheroid models 3D cell culture
Scientific posterPrimary human hepatocytes 3D in vitro culture model for studying hepatic function 3D cell culture
Scientific posterPrimary human hepatocyte 3D spheroids for studying hepatic function and drug toxicity (ISSCR Symposia 2019) 3D cell culture
Selection guideLipofectamine transfection reagent selection guideTransfection
Selection guideNunc cell culture surface selection guide Cultureware
Selection guideNunc microplate selection guide Cultureware
Selection guidePrimary cell media & supplement selection guideGeneral cell culture
Selection guideSelection guide for CRISPR delivery systemsTransfection
Selection guideiMATCH sera lot matching toolGeneral cell culture
Webinar24 Hours of Stem CellsGeneral cell culture
WebinarAvoid the icebergs! Managing sample preparation for cryo storage Cryopreservation
WebinarBest practices in cell counting for accurate and reproducible data Cell analysis
WebinarBest practices for incubating sensitive cells General cell culture
WebinarCell culture contamination: What you don't know can cost you General cell culture
WebinarChoosing the ideal lab water system for your cell culture applications General cell culture
WebinarChoosing the right cold storage for cell culture applications Cryopreservation
WebinarCulture your cells using less oxygen for biologically relevant results General cell culture
WebinarEliminate the threat: Cleaning and contamination in your biological safety cabinet Biological safety cabinet
WebinarFrom the hood to the microscope—Revolutionizing cell-based imaging Cell analysis
WebinarGet a handle on liquid handling. Increase pipetting efficiency for reproducible results General cell culture
WebinarGibco Cell Culture HeroesGeneral cell culture
WebinarHarnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly General cell culture
WebinarMaintaining integrity: Selecting the right storage tube for downstream cell culture Cryopreservation
WebinarNo more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells General cell culture
WebinarNurture your incubator; nurture your cells. Proper care and “feeding” of your cell culture incubator CO2 Incubation
WebinarOptimize your cell culture workflow with Thermo Scientific peristaltic pumps General cell culture
Webinar;Optimizing results in your downstream imaging applications Cell analysis
WebinarProtect you and your science—with biological safety cabinets Biological safety cabinets
WebinarSurface selection: Achieving optimal growth and harvesting of your cells Cultureware
WebinarTake your samples for a spin: Fundamentals of cell culture centrifugation General cell culture
WebinarTake your cell-based assays to the edge; reducing edge-effects for full-plate results Cultureware
WebinarThe last line of defense: cell culture contamination and using filtration as a final preventative measure General cell culture
WebinarThe power behind culturing cells on a porous membrane Cultureware
WebinarThe seven keys to safeguarding your cryopreserved cells Cryopreservation
WebinarUnderstanding all incubation parameters for sensitive cell growth CO2 Incubation
WebinarVersatile is smart! The power behind culturing cells on a porous membrane Cultureware

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