Cell Culture Cafe

Welcome to the Cell Culture Café, a science-based community where techniques, tips, and questions concerning cell culture are presented and discussed. Via interactive webinars, our cell culture experts explore topics ideally suited to your biggest concerns in the lab.

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Featured Cell Culture Café videos

Keeping your COincubator free from contamination

Choosing the right cold storage for cell culture

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Webinar title Webinar topics Featured products
Ultrapure water and cell culture: Know why, what, and how for proper water system maintenance Ultrapure water is an important staple to the cell culture lab. The water system is expected to deliver water that is free of impurities, of consistent quality and always available upon demand. This webinar investigates the different types of impurities in water and how water systems remove these critical impurities. We will also review guidelines for properly maintaining the lab water system to ensure successful cell culture. Water Purification Systems
In Search of the Perfect Pellet: Recommendations for Cell Culture Centrifugation The centrifuge is a crucial but often overlooked component within the cell culture workflow. Cultured mammalian cells are extremely delicate and require gentle centrifugation processing to maximize viable yield. Join us as we discuss important centrifuge considerations, to help increase efficiency and ergonomics, while reducing the risk of contamination. We will examine ways to help achieve optimal pellets and how to improve reproducibility between runs for high-quality results. Benchtop Centrifuges
Culture Cells Using Less Oxygen for Biologically Relevant Results Culture cells using less oxygen for biologically relevant results. Cell metabolism, proliferation, differentiation, disease progression and many more processes are modulated in response to oxygen concentration. Controlling oxygen in vitro is increasingly important in cancer and other disease modeling, drug discovery, toxicity testing, regenerative tissue development and biopharmaceutical production. CO2 Incubators
Avoid the icebergs! Managing sample preparation for cryo storage Determining the proper sample preparation technique for long term cryopreservation storage is of paramount importance to all downstream work stemming from the affected samples. During this webinar, we will discuss what happens during the sample preparation process, key concepts to be familiar with and key questions to consider when making decisions about long- term storage sample preparation. Cryopreservation Systems
Best practices in cell counting for accurate and reproducible data Accurate cell counting is a critical component for many biological applications. Without precise and reproducible cell counting and viability data, it is very difficult to determine if the root cause of downstream observations are due to cell culture and sample preparation or the intended experimental variables being tested. Automated Cell Counters
Cell culture contamination: What you don't know can cost you Cell culture use continues to increase beyond biological research with novel clinical applications in personalized medicine and cellular therapy. Cultivating these physiologically relevant cell types requires stringent conditions and heightens concerns regarding the perils of contamination. CO2 Incubators
Choosing the ideal lab water system for your cell culture applications Cell culture experiments are often sensitive to organic, inorganic, and bacterial contamination. Water must be free of these impurities to help ensure that you have consistent results with your experiments. We discuss water impurities and the technologies used to remove them. Water Purification Systems
Choosing the right cold storage for cell culture applications Learn how to select the best cold storage and sample prep solutions, with an eye to pertinent criteria that can lead to an optimized cell culture experience. Cold Storage
Choosing the right culture surface for your cells An overview of established and novel technologies used to modify cultureware surfaces for optimally growing adherent as well as non-adherent mammalian cells. Characterize the different Thermo Scientific™ Nunc™ surfaces by their adhesion properties and provide examples of how best to select the best culture surface for your application. Cell Culture Plastics
Eliminate the threat: Cleaning and contamination in your biological safety cabinet Contamination is the ever present threat to good science and your hard work. This webinar provides important points that will help you work with your biological safety cabinet more effectively to prevent contamination in your cell culture lab. Biological Safety Cabinets
From the hood to the microscope – Revolutionizing cell-based imaging Showcases some of the latest advancements in cell-based research and imaging. Learn as we explore how to obtain superior results through the careful selection of reagents and the optimization of your imaging workflow— from growing cells on suitable surfaces through to image capture. Particular attention will be given to growing and monitoring cells for imaging, fluorescent labeling of live cells, critical considerations for time-lapse imaging and optimizing live cell imaging. EVOS Cell Imaging Systems
Get a Handle on Liquid Handling. Increase pipetting efficiency for reproducible results One of the most common tools in any cell culture laboratory is a pipette. You turn to it time and time again, and like most tools, finding the right fit for your lab is important to enable accurate and precise transferring of samples. During this webinar, we will review common requirements for cell culture applications, and key considerations to provide worry-free pipetting for confidence in your results. Pipettes and Pipette Tips
Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across many different cell types, including transformed cell lines and pluripotent stem cells. Nunclon™ Sphera™ Microplates
How to choose the right Cold Storage Unit Learn how to select the best cold storage and sample prep solutions, with an eye to pertinent criteria that can lead to an optimized cell culture experience. Lab Refrigerators
Maintaining integrity: Selecting the right storage tube for downstream cell culture Ensuring storage integrity and traceability of cells is of particular importance for cell culture applications. This webinar will provide detail related to cell preservation and storage considerations, as well as criteria for selecting appropriate storage tubes to meet your downstream cell culture needs. Cryogenic Tubes
No more separation anxiety! A research case study: Gentle, non-enzymatic detachment of sensitive cells Application of an innovative surface to enable transfection and non-invasive detachment of macrophages. This research case study can be applied to diverse applications and cell types, offering insights into viable alternatives to your cell detachment methods. Nunc UpCell Surface
Nurture your incubator; nurture your cells. Proper Care and “feeding” of your cell culture incubator You trust your work—your cells--to your CO2 incubator. So if a problem occurs, it can be catastrophic for your research, setting you back weeks or months. This webinar provides important points that will help you avoid common mistakes, reduce contamination, and extend the life of your incubator. CO2 Incubators
Optimize your cell culture workflow with Thermo Scientific peristaltic pumps Optimizing cell culture processes is often dependent on how the cell culture fluid and media are handled. The proper selection of fluid handling equipment—pumps—can help improve efficiency and production in bio-processing research and production. Nalgene™ 50 Platinum-Cured Silicone Tubing for Peristaltic Pumps
Optimizing results in your downstream imaging applications Get the clear picture on your imaging applications workflow – from culturing and seeding your cells to image acquisition and data analysis - and how you can significantly improve your imaging results downstream by merely choosing the right culture vessel. Nunc™ Glass Bottom Dishes
Cell Culture Contamination: What You Don't Know Can Cost You Cell culture use continues to increase beyond biological research with novel clinical applications in personalized medicine and cellular therapy. Cultivating these physiologically relevant cell types requires stringent conditions and heightens concerns regarding the perils of contamination. CO2 Incubators
Protect you and your science with biological safety cabinets Effective use of the Class II biological safety cabinet (BSC) protects you and your important work. Explore how BSCs use effective air filtration and controlled airflow to prevent contamination from passing into the BSC. Biological Safety Cabinets
Surface selection: Achieving optimal growth and harvesting of your cells An overview of established and novel technologies used to modify cultureware surfaces for optimally growing adherent as well as non-adherent mammalian cells. We characterize the different Thermo Scientific™ Nunc™ surfaces by their adhesion properties and provide examples of how best to select the best culture surface for your application. We also present alternative methods to help improve cell harvesting. Nunc™ Surfaces
Take your samples for a spin: Fundamentals of cell culture centrifugation Separating cells is a critical step in the dynamic process of cell culture. This webinar will examine the role of centrifugation in the cell culture workflow and explore the different types of centrifuges used to separate cells. We will look at centrifuge requirements and technical considerations for cell culture applications, including selecting the appropriate speed and g-force. State-of-the-art trends in centrifugation will also be discussed. Benchtop Centrifuges
The last line of defense: Cell culture contamination and using filtration as a final preventative measure Filtration is the last line of defense against inadvertent contamination of cell culture media and buffers by bacteria, mycoplasma and particulates.In this webinar, we discuss cell culture contamination and the use of final filtration in the laboratory as a way to mitigate the risk of cell culture loss from contamination. Nalgene™ Rapid-Flow™ filters
Understanding all incubation parameters for sensitive cell growth In advanced biological applications such as pharmaceutical testing, regenerative medicine/cell therapy, and modeling of cancer, immunology, neurology etc., the integrity and health of cells are proportionally more important. To best support sensitive cells and to ensure they give accurate responses predictive of in vivo conditions, new CO2 incubators designed for 21st century cell culture offer better culturing environments than previously available. This presentation will help you understand how all incubation parameters work together to support cell health. CO2 Incubators

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