Cell Culture Flasks | Tissue Culture Flasks
Your choice for optimal scale-up
Find Thermo Scientific cell culture flasks designed for consistency, cell health and reproducibility, with the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a variety of sizes, with culture areas ranging from 25cm2 to 500cm2 and a selection of surface treated offerings to address your specific applications and cell types.
Quick links
- Single-Use PETG Erlenmeyer Flasks—Plain bottom
- Single-Use PETG Erlenmeyer Flasks—Baffled bottom
Nunc EasYFlasks treated cell culture flasks
- Available in sizes: T25, T75, T175 and T225.
- Wide, angled neck provides easy access to entire growth surface.
- Cell culture treated and lot-to-lot certified for adhesion cultures, to ensure consistency, reproducibility and reliability. Certificate included in every case.
- Cap opens with 1/3 turn allowing one-handed operation.
- Filter and vent/close cap options.
Order now:
Cell culture flasks for suspension cells
Made with high quality, optically clear virgin polystyrene, these non-treated cell culture flasks offer a high level sterility assurance ideal for suspension culture of non-adherent cells.
- Available in sizes: T25, T75, T175
- Wide, angled neck provides easy access to entire growth surface
- Filter cap opens with 1/3 turn allowing one-handed operation
- White cap for easy identification
Order now:
Specialty cell culture flasks
Enables formation of reproducible spheroid cultures
- Available in T25 and T75 sizes
- Intended for spheroid culture, organoid culture, and 3D culture
- Wide, angled neck provides easy access to entire growth surface
- Cell culture treated and lot-to-lot certified for adhesion cultures
- Cap opens with 1/3 turn allowing one-handed operation; available with filter caps
For cells with low adherence or growth
- Available in T25, T75, and T175 sizes
Poly-d-lysine (PDL) and collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.
The uniform coating creates a positive charge on the surface that helps ensure cell attachment, growth and differentiation. Should be used when cell lines are difficult to grow and show low adherence or slowed growth even after optimization of growth conditions.
Large-scale cell culture flasks
Largest single layer cell culture treated flask on the market.
- 1,900 mL total volume
- Large writing area
- Prominent stacking feet enable reliable stacking of multiple flasks
- Filter cap with 0.2 μm pore size hydrophobic membrane for sufficient gas exchange
Enables compact scale-up and automation
- Provides 500 cm2 surface area for cell attachment with the space-saving footprint of a T175 flask
- Expanded surface saves incubator space and increases productivity
- Treated for maximum adhesion of a broad range of cell types
- Available with a non-treated surface for suspension cultures
- Barcoded option available
Perfect your cell culture processes at every stage, from research and process development to large-scale biomanufacturing. The quality of our production cell culture platforms, including Thermo Scientific Nunc Cell Factory Systems, Nunc roller bottles, and Nalgene single-use shaker flasks, reflects the rigorous standards that have made us the world's leading provider of cell culture products.
What features should you consider when selecting a cell culture flask?
| Why EasYFlask is the optimal choice for cell culture and scale-up | |
|---|---|
| Neck width | Extra wide neck provides easier access to growth surface with scraper or pipette |
| Cap opening | 1/3-turn cap enables one-handed operation and avoids wrist strains |
| Volume graduations | Molded and printed for easy and quick measurement of growth media |
| Sterility assurance level (SAL) | Sterility assurance of SAL 10-6 guarantees that no more than one flask out of 1 million may be non-sterile |
| Lot-to-lot testing and certification in each case | Lot specific testing across four cell lines and primary cells ensures consistency, reproducibility and reliability of quality; certificate included in each case |
| Airflow below flask | Vented ribs on flask bottom enable optimal temperature equilibration |
| Robust design | Thick walls provide robust design for better resistance to mechanical stress |
| Continuity of surface for scale up | The Thermo Scientific Nunclon Delta surface—for maximum attachment and growth of adherent cultures—is offered across our T25, T75, T175, T225 and T500 flasks, enabling consistency and confidence when scaling up |
Related cell culture flasks categories
With more than 100 combinations of format and surface, our polystyrene cell culture plates help promote healthy cells and generate reliable results.
Maximum performance for a broad range of cell types and applications. Choose from round and flat-bottom tubes as well as conical tubes that offer a variety of sizes and features, including recyclable racks and ease-of-use designs for enhanced lab performance.
Browse a full line of dishes with various surface treatment options to promote adherent, suspension, and 3D spheriod cell cultures. Also available in glass bottom for high quality imaging applications.
Available in a wide range of surfaces and formats, choose Nunc Lab-Tek and Lab-Tek II Chamber Slides which enable seeding, incubating, fixing and staining on a single slide.
Designed and manufactured to provide the quality and consistency you need, Gibco media, sera and reagents help you achieve reproducible results and successful research outcomes—every day.
Advance your research with high-performing, proven cell culture instruments and equipment, including CO2 incubators, biological safety cabinets, general purpose centrifuges, lab refrigerators and freezers, automated cell counters, and cell imaging systems.
Simply select your criteria from the available options; the cell culture plate guide will return all plates that match your selections.
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.
Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.
Related products
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support? Contact our expert team for technical and application support of Laboratory Products
For Research Use Only. Not for use in diagnostic procedures.