Your choice for optimal scale-up

Find Thermo Scientific™ cell culture flasks designed for consistency, cell health and reproducibility, with the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a variety of sizes, with culture areas ranging from 25cm2 to 500cm2 and a selection of surface treated offerings to address your specific applications and cell types.

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Cell culture flasks for adherent cells

Nunc Cell Culture Treated EasYFlasks

  • Available in sizes: T25, T75, T175 and T225.
  • Wide, angled neck provides easy access to entire growth surface.
  • Cell culture treated and lot-to-lot certified for adhesion cultures, to ensure consistency, reproducibility and reliability. Certificate included in every case.
  • Cap opens with 1/3 turn allowing one-handed operation.
  • Filter and vent/close cap options.

Cell culture flasks for suspension cells

Nunc non-treated EasYFlask

Made with high quality, optically clear virgin polystyrene, these non-treated cell culture flasks offer a high level sterility assurance ideal for suspension culture of non-adherent cells.

  • Available in sizes: T25, T75, T175
  • Wide, angled neck provides easy access to entire growth surface
  • Filter cap opens with 1/3 turn allowing one-handed operation
  • White cap for easy identification

Specialty cell culture flasks

Enables formation of reproducible spheroid cultures

  • Available in T25 and T75 sizes
  • Intended for spheroid culture, organoid culture, and 3D culture
  • Wide, angled neck provides easy access to entire growth surface
  • Cell culture treated and lot-to-lot certified for adhesion cultures
  • Cap opens with 1/3 turn allowing one-handed operation; available with filter caps

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PDL flasks

For cells with low adherence or growth

  • Available in T25, T75, and T175 sizes

Poly-d-lysine (PDL) and collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.

The uniform coating creates a positive charge on the surface that helps ensure cell attachment, growth and differentiation. Should be used when cell lines are difficult to grow and show low adherence or slowed growth even after optimization of growth conditions.

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Large scale cell culture flasks

T300 flasks

Find the largest single layer cell culture treated flask on the market with a 1,900mL total volume and bold printed graduations from 100 to 500mL.

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Nunc TripleFlasks

Enables compact scale-up and automation

  • Provides 500 cm2 surface area for cell attachment with the space-saving footprint of a T175 flask
  • Expanded surface saves incubator space and increases productivity
  • Treated for maximum adhesion of a broad range of cell types
  • Available with a non-treated surface for suspension cultures
  • Barcoded option available

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Nunc™ EasyFill™ Cell Factory™ Systems

Perfect your cell culture processes at every stage, from research and process development to large-scale biomanufacturing. The quality of our production cell culture platforms, including Thermo Scientific™ Nunc™ Cell Factory™ Systems, Nunc™ roller bottles, and Nalgene™ single-use shaker flasks, reflects the rigorous standards that have made us the world's leading provider of cell culture products.

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What factors should you consider when selecting a cell culture flask?

  Why EasYFlask is the optimal choice for cell culture and scale-up
Neck width Extra wide neck provides easier access to growth surface with scraper or pipette
Cap opening 1/3-turn cap enables one-handed operation and avoids wrist strains
Volume graduations Molded and printed for easy and quick measurement of growth media
Sterility assurance level (SAL) Sterility assurance of  SAL 10-6 guarantees that no more than one flask out of 1 million may be non-sterile
Lot-to-lot testing and certification in each case Lot specific testing across four cell lines and primary cells ensures consistency, reproducibility and reliability of quality; certificate included in each case
Airflow below flask Vented ribs on flask bottom enable optimal temperature equilibration
Robust design Thick walls provide robust design for better resistance to mechanical stress
Continuity of surface for scale up The Thermo Scientific™ Nunclon™ Delta surface—for maximum attachment and growth of adherent cultures—is offered across our T25, T75, T175, T225 and T500 flasks, enabling consistency and confidence when scaling up

Related cell culture flasks categories

With more than 100 combinations of format and surface, our polystyrene cell culture plates help promote healthy cells and generate reliable results.

Maximum performance for a broad range of cell types and applications. Choose from round and flat-bottom tubes as well as conical tubes that offer a variety of sizes and features, including recyclable racks and ease-of-use designs for enhanced lab performance.

Browse a full line of dishes with various surface treatment options to promote adherent, suspension, and 3D spheriod cell cultures. Also available in glass bottom for high quality imaging applications.

Available in a wide range of surfaces and formats, choose Nunc Lab-Tek and Lab-Tek II Chamber Slides which enable seeding, incubating, fixing and staining on a single slide.

Designed and manufactured to provide the quality and consistency you need, Gibco media, sera and reagents help you achieve reproducible results and successful research outcomes—every day.

Advance your research with high-performing, proven cell culture instruments and equipment, including CO2 incubators, biological safety cabinets, general purpose centrifuges, lab refrigerators and freezers, automated cell counters, and cell imaging systems.

Select the ideal plate for your application

Simply select your criteria from the available options; the guide will return all plates that match your selections.

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Related flask and cell culture resources

Surface selection: Achieving optimal cell growth and harvesting

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

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Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly

Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across different cell types.

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Featured article

Cell adhesion and growth on coated or modified glass or plastic surfaces

Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge. 

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