T175 Flasks

Your choice for optimal scale-up

Continue your scale-up with a T175 flask that has the design and surface options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options, and surface treatment alternatives that help to ensure your healthy tissue cells expand and grow in the flask of your choice.

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What makes our Nunc EasYFlasks so easy to use?

Angled neck TC-treated

Angled neck non-treated

Filter or vent/close cap

EasYFlasks

Ergonomic design for easy access to the growth surface by pipettes or cell scrapers
Low profile to maximize incubator space
Slightly angled side walls for complete viewing under a microscope
Volume graduations printed and molded on both sides
Sterilized by gamma irradiation and certified to be non-pyrogenic

Filter caps

Effective barrier against micro-organisms
Continuous venting with consistent gas exchange

Vent/close caps

“Y” mark allows visual verification of vent position: open or closed

Find your T175 cell culture flask by surface

To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. Let us help guide your product selection with specific cell types and desired cell culture ranges in mind.

For additional guidance on usage by cell type, view our Surface Selection Guide .

Adherent cell culture vs. suspension cell culture
There are two basic systems for growing cells in culture: as monolayers on an artificial substrate (i.e., adherent culture), or free-floating in the culture medium (suspension culture). Visit our technical reference library for an introduction to cell culture and to take a closer look at adherent cell culture vs. suspension cell culture.

The Nunclon Delta cell culture surface
The Thermo Scientific Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific cell culture plastics.

T175 flasks for adherent cells

The proprietary Nunclon Delta cell culture surface supports optimal growth and attachment of cells to the growth surface.

	Neck	Surface	Material	Cap	Cap Feature
EasYFlask	Angled	Nunclon Delta	Cell culture-treated polystyrene	Filter	Constant airflow
EasYFlask	Angled	Nunclon Delta	Cell culture-treated polystyrene	Vent/close	Sufficient gas exchange

We also have regular Thermo Scientific Nunc Cell Culture Treated Flasks with straight necks and filtered caps or vent/close caps.

T175 flasks with poly-d-lysine coating or collagen I coating

Poly-D-Lysine (PDL) and collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. Selection of one surface over another depends on tissue type. We suggest reviewing previous references for your specific cell line or testing both, if necessary.

	Neck	Surface	Description	Cap
PDL-Coated EasYFlask	Angled	Poly-D-lysine	Animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach	Filter
Collagen I-Coated EasYFlask	Angled	Collagen I	Surface of animal origin; for adherent cultures of primary cells that are finicky and hard to attach	Filter

T175 flasks for suspension culture of non-adherent cells

Made with high quality, optically clear virgin polystyrene, the Thermo Scientific Nunc Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.

	Neck	Surface	Description	Cap
Non-treated EasYFlask 2/pack	Angled	Not cell culture treated	Untreated polystyrene for suspension growth of non adherent cells	Filter

Related flask and cell culture resources

Surface selection: Achieving optimal cell growth and harvesting (webinar)

Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.

Surface modifications to improve cell adhesion
Characterization of Nunc cell culture surfaces by adhesion properties
Alternative methods for enzyme-free cell harvesting

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Featured article

Cell adhesion and growth on coated or modified glass or plastic surfaces

Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.

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