Your choice for optimal scale-up
Continue your scale-up with a T175 flask that has the design and surface options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options, and surface treatment alternatives that help to ensure your healthy tissue cells expand and grow in the flask of your choice.
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What makes our Nunc EasYFlasks so easy to use?
Angled neck TC-treated
Angled neck non-treated
Filter or vent/close cap
EasYFlasks
- Ergonomic design for easy access to the growth surface by pipettes or cell scrapers
- Low profile to maximize incubator space
- Slightly angled side walls for complete viewing under a microscope
- Volume graduations printed and molded on both sides
- Sterilized by gamma irradiation and certified to be non-pyrogenic
Filter caps
- Effective barrier against micro-organisms
- Continuous venting with consistent gas exchange
Vent/close caps
- “Y” mark allows visual verification of vent position: open or closed
To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. Let us help guide your product selection with specific cell types and desired cell culture ranges in mind.
For additional guidance on usage by cell type, view our Surface Selection Guide .
Adherent cell culture vs. suspension cell culture
There are two basic systems for growing cells in culture: as monolayers on an artificial substrate (i.e., adherent culture), or free-floating in the culture medium (suspension culture). Visit our technical reference library for an introduction to cell culture and to take a closer look at adherent cell culture vs. suspension cell culture.
The Nunclon Delta cell culture surface
The Thermo Scientific Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific cell culture plastics.
The proprietary Nunclon Delta cell culture surface supports optimal growth and attachment of cells to the growth surface.
We also have regular Thermo Scientific Nunc Cell Culture Treated Flasks with straight necks and filtered caps or vent/close caps.
Poly-D-Lysine (PDL) and collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. Selection of one surface over another depends on tissue type. We suggest reviewing previous references for your specific cell line or testing both, if necessary.
| Neck | Surface | Description | Cap | |
|---|---|---|---|---|
| PDL-Coated EasYFlask | Angled | Poly-D-lysine | Animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach |
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| Collagen I-Coated EasYFlask | Angled | Collagen I | Surface of animal origin; for adherent cultures of primary cells that are finicky and hard to attach |
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Made with high quality, optically clear virgin polystyrene, the Thermo Scientific Nunc Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.
| Neck | Surface | Description | Cap | |
|---|---|---|---|---|
| Non-treated EasYFlask 2/pack | Angled | Not cell culture treated | Untreated polystyrene for suspension growth of non adherent cells |
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Surface selection: Achieving optimal cell growth and harvesting (webinar)
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for enzyme-free cell harvesting
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
Surface selection: Achieving optimal cell growth and harvesting (webinar)
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for enzyme-free cell harvesting
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
For Research Use Only. Not for use in diagnostic procedures.
