T75 Flasks
Your choice for optimal scale-up
Start your scale-up with a T-75 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help to ensure your healthy tissue cells expand and grow in the flask of your choice.
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What makes our Nunc EasYFlasks so easy to use?
Angled neck
EasYFlasks
- Ergonomic design for easy access to the growth surface by pipettes or cell scrapers
- Low profile to maximize incubator space
- Slightly angled side walls for complete viewing under a microscope
- Volume graduations printed and molded on both sides
- Sterilized by gamma irradiation and certified to be non-pyrogenic
Filter or vent/close cap
Filter caps
- Effective barrier against micro-organisms
- Continuous venting with consistent gas exchange
Vent/close caps
- “Y” mark allows visual verification of vent position as either open or closed
Find your T75 cell culture flasks by surface
To help ensure flexible, reproducible and reliable results across every stage of cell culture, we offer a range of cell culture flasks spanning a variety of formats, sizes and surfaces. Let us help guide your product selection with specific cell types and desired cell culture ranges in mind.
For additional guidance on usage by cell type, view our Surface Selection Guide (PDF).
Adherent cell culture vs. suspension cell culture
There are two basic systems for growing cells in culture: as monolayers on an artificial substrate (i.e., adherent culture) or free-floating in the culture medium (suspension culture). Visit our technical reference library for an introduction to cell culture and to take a closer look at adherent cell culture vs. suspension cell culture.
The Thermo Scientific™ Nunclon™ Delta cell culture surface
The Nunclon Delta surface is a fully synthetic energy-treated surface that makes the otherwise very hydrophobic polystyrene surface more hydrophilic, thus facilitating cell attachment and growth. The Nunclon Delta certificate ensures high quality standards and lot-to-lot consistency. Look for it on a variety of Thermo Scientific™ cell culture plastics.
The proprietary Nunclon Delta cell culture surface supports optimal growth and attachment of cells to the growth surface.
We also have regular T80 flasks with straight necks in our Thermo Scientific™ Nunc™ Cell Culture Treated Flasks with filter caps or vent/close caps.
Make your most valuable work your best work with Thermo Scientific™ Nunclon™ Sphera
Elevate your stem cell and cancer research with a surface that supports many different cell types and their ability to generate spheroids. Studies show that the Nunclon Sphera surface enables consistent, repeatable spheroid formation and growth.
| Neck | Surface | Description | Cap | |
|---|---|---|---|---|
| Nunclon Sphera Flask | Angled | Nunclon Sphera | Set in the EasYFlask format, the Nunclon Sphera Flasks enable 3D scaffold-free spheroid culture (e.g. tumor spheroids, embryoid body formation of pluripotent stem cells) | Filter |
Poly-D-Lysine (PDL) and Collagen I coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface. Selection of one surface over another depends on tissue type; we suggest reviewing previous references for your specific cell line or testing both, if necessary.
| Neck | Surface | Description | Cap | |
|---|---|---|---|---|
| PDL Cell Culture Coated EasYFlask | Angled | Poly-D-Lysine | Animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach |
Filter |
| Collagen I Cell Culture Coated EasYFlask | Angled | Collagen I | Surface of animal origin; for adherent cultures of primary cells that are finicky and hard to attach |
Filter |
Made with high quality, optically clear virgin polystyrene, the Thermo Scientific™ Nunc™ Non-treated Flasks, with high level sterility assurance, are ideal for suspension culture of non-adherent cells.
| Neck | Surface | Description | Cap | |
|---|---|---|---|---|
| Non-treated EasYFlask | Angled | Not cell culture treated | Untreated polystyrene for suspension growth of non adherent cells |
Filter |
Related flask and cell culture resources
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for advanced cell harvesting
Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly
Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across different cell types.
- Closely simulate tumor progression in vitro
- Assess efficacy of anti-cancer agents
- Generate embryoid bodies for germ layer differentiation
- Form 3D cellular structures for many cell types
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for advanced cell harvesting
Harnessing new dimensions in your research: Growing 3D cultures consistently and repeatedly
Learn about a novel surface technology that enables consistent, repeatable growth of spheroid cultures across different cell types.
- Closely simulate tumor progression in vitro
- Assess efficacy of anti-cancer agents
- Generate embryoid bodies for germ layer differentiation
- Form 3D cellular structures for many cell types
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
For Research Use Only. Not for use in diagnostic procedures.
