Kanamycin Sulfate is effective as a bacteriocidal agent by inhibiting ribosomal translocation and eliciting miscoding. Resistance is conferred by the KanR-Tn5 gene product (aminoglycoside phosphotransferase), which modifies the antibiotic and prevents interaction with ribosomes. Liquid kanamycin (100X) contains 10 mg/ml kanamycin (base) utilizing kanamycin sulfate in 0.85% saline.
Keep your irreplaceable cultures contamination freeResearchers are typically concerned about two types of cell culture contamination: the contamination of cell cultures with microbiological organisms and the contamination of one cell line with another. Both types of contamination are extremely prevalent, and the possibility of contamination—and the impact on results—cannot be underestimated. The decision to use antibiotics and antimycotics to prevent contamination should be based on the individual researcher’s needs and experience.
We manufacture a wide array of antibiotics and antimycotics to control contamination arising from bacteria, fungi, mycoplasma, and yeast. While researchers may attempt to eliminate or control contaminations with antibiotics or antimycotics, it should be noted that these substances can be toxic to certain cell lines. Before using, it is essential to first perform a dose-response test to determine the level at which toxicity begins.