Gibco universal cell culture bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, without the need for costly customization.
Explore the many benefits of using Gibco cell and tissue culture bags in your lab:
Figure 4. Four-port design of Gibco culture bag.
Gibco universal cell culture media bags are available with multiple mechanical connection methods that offer:
The product contact layer of the Gibco universal cell culture media bag is ultralow density polyethylene and has the following benefits:
|Physical property||Dimension||Value (before/after gamma sterilization)*||Procedure|
|Haze||%||5 / 5||ASTM D-1003|
|Clarity||%||98 / 98||ASTM D-1003|
|Transmittance||%||93 / 93||ASTM D-1003|
|Tensile strength at break||MPa||14 / 13||ASTM D-882|
|Elongation at break||%||280 / 300||ASTM D-882|
|Elastic modulus||MPa||370 / 350||ASTM D-882|
|Break at cold temperature||ºC||Below –45 / below –45||ISO 8570|
|Water vapor transmission rate**||g/(m2.day)||0.35 / 0.32||ASTM F-1249|
|Oxygen permeability**||cm3/(m2.day.atm)||<0.05 / <0.05||ASTM D-3985|
|Carbon dioxide permeability**||cm3/(m2.day.atm)||<0.2 / <0.2||ASTM F-2476|
|Film thickness||0.325 mm|
|*Gamma sterilization dose 25 kGy (except parameters marked ** received 50 kGy)|
|Appearance of solution S1||Pass||Pass|
|Acidity or alkalinity||≤1.5 mL 0.01 N NaOH|
≤1.0 mL 0.01 N HCl
|Pass (0.60 mL)|
Pass (0.40 mL)
|UV absorbance (220-340 nm)||≤0.20||Pass (0.01)|
|Reducing substances||≤0.5 mL||Pass (0.20 mL)|
|Extractable aluminum||≤1 ppm||Pass|
|Extractable zinconium||0.0||≤ 0.1 ppm|
|Extractable chromium||≤0.05 ppm||Pass|
|Extractable heavy metals||≤2.5 ppm||Pass|
|Extractable titanium||≤1 ppm||Pass|
|Extractable vanadium||≤0.1 ppm||Pass|
|Extractable zinc||≤1 ppm||Pass|
|Extractable zirconium||≤0.1 ppm||Pass|
|Sulphated ash||≤1%||Pass (0.02%)|
|Substances soluble in hexane||NA||Impracticable|
|Antioxidants (max. 3)||Pass||Pass|
|Amides and stearates||Pass||Pass|
|Nonvolatile residue||USP <661>||≤15 mg||Pass|
|Residue on ignition||USP <661>||≤5 mg||Pass|
|Heavy metals||USP <661>||<2 ppm||Pass|
|Buffering capacity||USP <661>||≤10 mL||Pass|
|Elution test||USP <87>||Pass||Pass|
|Systemic injection test||USP <88>||Class VI||Pass|
|Intracutaneous test||USP <88>||Class VI||Pass|
|Buffering capacity||USP <88>||Class VI||Pass|
|Lower is better|
The Gibco PE film demonstrates significantly lower gas transmission rates in comparison to other cell and tissue culture bags on the market.
|Figure 1.Gas transmission rates of Gibco PE film compared to other culture bags on the market.|
|Figure 2.Structure of Gibco PE film.|
A shelf life stability study was conducted over a 19 month period for CD-CHO, DMEM, and FreeStyle 293. Media packaged in the Gibco PE film remains within pH specification limits.
Figure 3.Results of shelf life stability study examining pH in CD-CHO, DMEM, and FreeStyle 293 media packaged in Gibco PE film.
Our knowledgeable packaging experts and technical specialists are ready to assist you in:
In addition, explore our media customization services for information on customized components, packaging, and QC testing.
Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, ensuring the consistency, reliability, and high quality you can rely on.
We are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.
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For Research Use Only. Not for use in diagnostic procedures.