Bottle containing DMEM F12 media

Culture your cells with Gibco DMEM F12

Gibco Dulbecco's Modified Eagle Medium: Nutrient Mixture F12 (DMEM F12) is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F12 media, this formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with the wide variety of components found in Ham’s F12 media.

Gibco DMEM F12 is formulated for superior quality and delivers greater reliability, consistency, and improved control in mammalian cell culture.

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DMEM F12 composition

Along with the glucose, amino acids, and vitamins in DMEM, DMEM F12 contains additional components found in Ham’s F12 media, including zinc, putrescine, hypoxanthine, and thymidine. DMEM F12 may also include other factors such as L-glutamine and phenol red indicator. However, due to its lack of proteins, lipids, and growth factors, DMEM F12 is commonly supplemented with fetal bovine serum (FBS). In addition, the sodium bicarbonate buffer system used in DMEM F12 requires a 5–10% CO2 environment for maintenance of physiological pH.

Thermo Fisher Scientific offers a variety of DMEM F12 modifications for a range of cell culture applications.

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DMEM alone is rich in nutrients such as amino acids and vitamins, while Ham’s F12 media contains a higher diversity of components. Therefore, DMEM F12 is a more complex media than DMEM, including many additional factors (e.g., zinc, thymidine) that are generally not found in more standard basal media formulations. 

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Choose the right DMEM F12

There are several factors to consider when selecting the right Gibco DMEM F12 composition for your cell culture experiments, including format, supplementation, and additives. Our interactive media formulation tool provides side-by-side comparisons of DMEM F12 formulations, allowing for easy selection of the DMEM F12 you need. Select the following modifications:

  • L-glutamine/GlutaMAX supplementation
  • Phenol red indicator
  • Liquid vs powder formats
  • Serum supplementation
  • Sodium pyruvate additive
  • Glucose additive
  • HEPES additive

Take your cell culture to the next level with enhanced DMEM formulations. Explore the following formulations that can help improve your cell viability, serum supplementation, cold storage constraints, and clinical cell culture.

Bottle of Advanced DMEM F12

Advanced DMEM F12

Gibco Advanced DMEM F12 includes ingredients that allow you to reduce FBS supplementation by 50–90%. This both helps reduce your costs and increase consistency in your experiments with fewer lot-to-lot serum changes.

BenchStable DMEM F12 product photo

BenchStable DMEM F12

Gibco BenchStable DMEM F12 is formulated for storage at room temperature, helping save valuable cold storage space while maintaining the ability to support cell proliferation and densities at levels comparable to standard DMEM F12.

Bottle of DMEM F12 with GlutaMAX Supplement

DMEM F12 with GlutaMAX supplement

Gibco DMEM F12 with GlutaMAX supplement allows you to provide your cells with a stable source of L-glutamine that does not spontaneously degrade, helping improve cell health and viability by reducing toxic ammonia buildup.

CTS Knockout DMEM F12

Taking your work to the clinic? Gibco Cell Therapy Systems (CTS) Knockout DMEM F12 is a cGMP manufactured formula designed for culturing human embryonic stem cells and induced pluripotent stem cells. 

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Customize your DMEM F12

If you require a custom DMEM F12 formulation, use our media customization services to select the DMEM F12 composition that is right for you, including custom media components, packaging, and QC testing.

Explore services for DMEM F12 customization

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Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.

Learn more about GMP manufacturing

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The 1 L Gibco bottle uses 38% less source material (32% less material for the 500 mL bottle) than comparable media bottles on the market today. This helps reduce the consumption of non-renewable resources, helping shrink your footprint.

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