Gibco Minimum Essential Media (MEM), patterned after Eagle’s Media, is one of the most commonly used of all cell culture media and is well suited for the growth of a broad spectrum of mammalian cells. Compared to the earlier Basal Medium Eagle (BME), MEM is formulated with increased concentrations of amino acids. Today there are many other MEM formulations available, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification.
MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.
Minimum Essential Medium (MEM) contains a higher concentration of amino acids than Basal Medium Eagle (BME). MEM media may be formulated with Earle’s salts for maintenance of pH in a CO2 incubator, or Hanks’ salts for maintenance of pH in atmospheric conditions. Because MEM media lacks proteins, lipids, or growth factors, it commonly requires supplementation with fetal bovine serum (FBS).
Thermo Fisher Scientific offers a variety of MEM modifications for a range of cell culture applications.
There are several factors to consider when selecting the right Gibco MEM composition for your cell culture experiments, including format, supplementation, and additives. Our interactive media formulation tool provides side-by-side comparisons of MEM media formulations, allowing for easy selection of the MEM you need. Select the following modifications:
Take your cell culture to the next level with enhanced MEM formulations. Explore the following formulations that can help improve your cell viability, serum supplementation, transfection experiments, and cold storage constraints.
Gibco Advanced MEM is formulated with ingredients that allow for the reduction of serum supplementation by 50–90%, supporting cell growth while lowering your costs and reducing variability caused by lot-to-lot serum changes.
If you require a custom MEM formulation for your cell culture experiments, our media customization services can provide you with the media you need, including custom components, packaging, and QC testing.
Gibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.
The 1 L Gibco bottle uses 38% less source material (32% less material for the 500 mL bottle) than comparable media bottles on the market today. This helps reduce the consumption of non-renewable resources, helping shrink your footprint.
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