Gibco Cell Culture Media

Superior quality and consistency for results you can rely on

Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We’ve developed ready-to-use cell culture media products as well as powdered and concentrated liquid formulations to fit your experimental setup and your budget.

Classic Gibco cell culture media

We are dedicated to providing quality Gibco cell culture media products that meet all of your research needs.

DMEM

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

RPMI 1640

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

MEM

MEM (Minimum Essential Medium) can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Try Opti-MEM I Reduced-Serum Medium an improved formulation that allows for at least 50% reduction of FBS supplementation with no change in growth rate or morphology.

IMDM

IMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells.

DMEM/F-12

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

Upgrade your cell culture media

Take your cell culture to the next level with enhanced formulations of your everyday favorites. Choose Gibco quality for consistent and reproducible results.

GlutaMAX media

  • Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
  • High viability for long-term cultures

Advanced media

  • Reduced-serum media to lower cost of serum by 50–90%
  • Greater consistency with fewer lot-to-lot serum changes

Recovery media

  • Improved cell recovery and viability after thawing from cryostorage
  • Ready-to-use; no need to combine multiple products to freeze cells

Additional cell culture media products

Gibco Cell Culture Basics

Cell culture is critical to your success, so make sure everyone in your lab uses the best procedures and products to consistently get the outcomes you expect. Cell Culture Basics is an introduction to cell culture, covering topics such as laboratory set-up, safety and aseptic technique.

Learn more

Resources

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

Related products

Support

Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

Need technical support? Contact our expert team for technical and application support of Laboratory Products