Gibco Cell Culture Media Family

Superior quality and consistency for results you can rely on

Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We’ve developed ready-to-use cell culture media products as well as powdered and concentrated liquid formulations to fit your experimental setup and your budget. Use the quick links below to access Gibco media.

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NEW Gibco BenchStable Media

media in ice cube

The coolest new media are totally uncool

Gibco BenchStable Media has been engineered for flexibility and convenience, enabling storage at room temperature. Save your lab's valuable cold storage space.

Check out the latest innovation in cell culture media

Classic Gibco cell culture media

We are dedicated to providing quality Gibco cell culture media products that meet all of your research needs.

DMEM (Dulbecco's Modified Eagle Medium)

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.


MEM (Minimum Essential Medium) can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. Try Opti-MEM I Reduced-Serum Medium for greater transfection efficiency. This improved formulation allows for at least 50% reduction of FBS supplementation with no observable change in growth rate or morphology.

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

Bottle Weight

The Gibco media bottle has its own bottle weight for keeping order in the water bath. The bottle weight fits both the 500 mL and 1 L bottles, keeping them upright in the water bath further reducing the risk of contamination.

Upgrade your cell culture media

Take your cell culture to the next level with enhanced formulations of your everyday favorites. Choose Gibco quality for consistent and reproducible results.

GlutaMAX media

  • Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
  • High viability for long-term cultures

Advanced media

  • Reduced-serum media to lower cost of serum by 50–90%
  • Greater consistency with fewer lot-to-lot serum changes

FluoroBrite media

  • Reduces background fluorescence in cell imaging
  • DMEM-based to preserve cell viability

Additional cell culture media products

Cell Culture Media Best Practices

Here are a few simple tips & tricks to help ensure your cell culture media is maintained for optimal performance.

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If refrigerated, warm your cell culture media before use. Your cells would be cold at 2-8°C, and you would be too!

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Keep your cell culture media protected from light. Light exposure degrades the essential vitamins in media that your cells need to grow.

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Once you have supplemented your cell culture media with FBS, place the complete media into the refrigerator (2-8°C ) to maintain performance.

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Use your supplemented media within 2-4 weeks to reduce the chances of contamination and the impact of pH drift.

leaf icon Less waste

When minimizing waste in your lab, every little bit helps. The 1 L Gibco bottle uses 38% less source material (32% less for the 0.5 L bottle) than comparable media bottles on the market today. This reduces the consumption of non-renewable resources helping shrink your footprint.
Download the Green Fact Sheet
Learn more about sustainable solutions

cell culture media icon GMP manufacturing

Gibco cell culture products are manufactured in facilities that are compliant with current good manufacturing practices (GMP) for medical devices and adheres to a robust quality management system.


Learn more about GMP manufacturing


Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

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Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

Need technical support? Contact our expert team for technical and application support of Laboratory Products