DMEM/F-12 Dulbecco’s Modified Eagle Medium F-12

Gibco Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) is formulated for superior quality, delivers greater reliability, consistency and improved control in mammalian cell culture.

DMEM/F-12 is a widely used basal medium for supporting the growth of many different mammalian cells including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. A 1:1 mixture of DMEM and Ham's F-12, this formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

We offer a variety of DMEM/F-12 modifications for a range of cell culture applications.

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Choose the right DMEM/F-12

Select the right Gibco MEM for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:

  • Glutamine/GlutaMAX supplementation
  • Phenol red indicator
  • Liquid vs powder format
  • Serum supplementation
  • Sodium pyruvate additive
  • Application

Specialty DMEM/F12 formulations

Take your cell culture to the next level with enhanced formulations of your everyday favorites.

  • Reduced-serum media to lower cost of serum by 50–90%
  • Greater consistency with fewer lot-to-lot serum changes

Choose Advanced DMEM/F12

  • Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
  • High viability for long-term cultures

Choose DMEM/F12 with GlutaMAX

Need a custom DMEM/F12 formulation? Customize your DMEM/F-12 media online

Taking your work to the clinic? Be sure to check out the CTS KnockOut DMEM/F-12

Find the right Gibco media formulation

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Use our interactive Media Formulation tool to compare basal media side-by-side. First choose your media, and then choose specific modifications to find the right match for your experiment. Easily compare selected media with the download feature for your future reference. 
View formulations


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