A key challenge of live-cell fluorescence microscopy is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. We have addressed this problem by developing Gibco FluoroBrite DMEM, a DMEM-based formulation with background fluorescence that is 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX, FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores so that researchers can visualize even the weakest fluorescent events in an environment that promotes optimum cell health.
- Enhancement of fluorescence signal during live-cell imaging
- DMEM-based to help preserve cell health