A key challenge of live-cell fluorescence microscopy is the ability to image weak fluorophores without causing cell damage, photobleaching, or undesirable changes to cell health. We have addressed this problem by developing Gibco FluoroBrite DMEM, a DMEM-based formulation with background fluorescence that is 90% lower than that emitted by standard phenol red–free DMEM. Formulated to include the required nutrients for routine cell culture when supplemented with 10% fetal bovine serum and 4 mM L-glutamine or GlutaMAX, FluoroBrite DMEM is designed to enhance the signal-to-noise ratio of fluorophores so that researchers can visualize even the weakest fluorescent events in an environment that promotes optimum cell health.
- Enhancement of fluorescence signal during live-cell imaging
- DMEM-based to help preserve cell health
FluoroBrite DMEM testimonials
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Figure 1. FluoroBrite DMEM has 10% of the background fluorescence emitted by standard phenol red–free DMEM and provides a 9-fold enhancement in signal-to-noise ratio.(A) Images of live epithelial cells that have been labeled with CellLight® Golgi-GFP, BacMam 2.0 (Cat. No. C10592) and cultured in phenol red–free DMEM (Cat. No. 31053) or FluoroBrite DMEM with 10% fetal bovine serum. Cells in 10X images are co-labeled with CellLight Actin-RFP, BacMam 2.0 (Cat. No. C10583). Cells in 40X images (inset) are co-labeled with ER-Tracker Red (BODIPY TR Glibenclamide; Cat. No. E34250). (B) Fluorescence signal from PBS, FluoroBrite DMEM, and phenol red–free DMEM at 509 nm in response to excitation at 480 nm. (C) Fluorescent signal fromdextran labeled with Alexa Fluor 488 dye (Cat. No. D22910) over background in PBS, FluoroBrite DMEM, and phenol red–free DMEM.
Figure 2. Cell lines cultured in FluoroBrite DMEM and standard DMEM display comparable long-term growth over multiple passages. Graphs display the average viable cell density (solid line; left y axis) and average percent viability (dotted line; right y axis) for three cell lines cultured for several passages in a standard phenol red–free DMEM (Cat. No. 31053) or FluoroBrite DMEM, both supplemented with 10% fetal bovine serum and and 4 mM GlutaMAX supplement. Note: the Sp2 suspension cell line was tested for long-term growth in FluoroBrite DMEM because of its known hypersensitivity to nutrient deficiencies.
- D’Souza et. al (2019). Defects in COG-Mediated Golgi Trafficking Alter Endo-Lysosomal System in Human Cells
- shows beautiful Golgi staining with the use of FluoroBrite DMEM medium
- Aldeghaither et. al (2018). A mechanism of resistance to Antibody-targeted immune attack
- assay for NK conjugation to target cells to study resistance to antibody mediated therapy
- Blinova et. al (2019). Clinical trial in a dish: personalized stem cell derived cardiomyocyte assay compared to clinical trial results of two QT prolonging drugs
- drug dilutions and dosing, action potential measurements in stem cell derived cardiomyocyte to assess for clinical trial results
- Ziemba et. al (2018). A PKC-MARCKS-PI3K regulatory module links Ca2+ and PIP3 signals at the leading edge of polarized macrophages
- study of cell polarity and chemotaxis of leukocytes
- Ali et. al (2019). Single-cell screening of Tamoxifen abundance and effect using Mass spectrometry and Raman Spectroscopy
- Steinmark et. al (2019). Targeted fluorescence lifetime probes reveal responsive organelle viscosity and membrane polarity
- study of organelle viscosity and membrane fluidity which can influence biological processes and contribute to diseases like Alzheimer’s disease and Leigh’s syndrome
- Lindstrom et. al (2018). Progressive recruitment of mesenchymal progenitors reveals a time-dependent process of cell fate acquisition in mouse and human nephrogenesis
- cell recruitment and cell fate decision in nephrogenesis
- Bianchi et. al (2019). Membrane mechanical properties regulate the effect of strain on spontaneous electrophysiology in human iPSC-derived neurons
- study of electrophysiology of human iPSC derived neurons
- Salgado et. al (2018). Visualization of calcium ion loss from Rotavirus during cell entry
- Bianchi et. al (2019). Engineering a uniaxial substrate-stretching device for simultaneous electrophysiological measurements and imaging of strained peripheral neurons
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