RPMI 1640 media

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.

We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.

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Choose the right RPMI 1640 Media

Select the right Gibco MEM for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:

  • Glutamine / GlutaMAX supplementation
  • Phenol red Indicator
  • Liquid vs powder format
  • Serum supplementation
  • Sodium pyruvate additive
  • Application

Browse the RPMI 1640 Media catalog

Specialty RPMI 1640 media formulations

Take your cell culture to the next level with enhanced formulations of your everyday favorites.

  • Reduced-serum media to lower cost of serum by 50–90%
  • Greater consistency with fewer lot-to-lot serum changes

Choose Advanced RPMI 1640

  • Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
  • High viability for long-term cultures

Choose RPMI 1640 with GlutaMAX
Choose RPMI 1640 with GlutaMAX and HEPES

Need a custom RPMI 1640 Media formulation? Customize your RPMI 1640 Media online


Find the right Gibco media formulation

screenshot of Cell Culture Media Formulation Tool

Use our interactive Media Formulation tool to compare basal media side-by-side. First choose your media, and then choose specific modifications to find the right match for your experiment. Easily compare selected media with the download feature for your future reference. 
View formulations

Resources

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References