Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium.
We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications.
NEW Gibco BenchStable Media
The coolest new media are totally uncool
Gibco BenchStable Media has been engineered for flexibility and convenience, enabling storage at room temperature. Save your lab's valuable cold storage space.
Choose the right RPMI 1640 Media
Select the right Gibco MEM for your cell culture. Browse the catalog and you’ll be able to narrow the list according to:
- Glutamine / GlutaMAX supplementation
- Phenol red Indicator
- Liquid vs powder format
- Serum supplementation
- Sodium pyruvate additive
- Application
Specialty RPMI 1640 media formulations
Take your cell culture to the next level with enhanced formulations of your everyday favorites.
- Reduced-serum media to lower cost of serum by 50–90%
- Greater consistency with fewer lot-to-lot serum changes
- Pre-supplemented with stable form of L-glutamine to prevent ammonia buildup
- High viability for long-term cultures
Choose RPMI 1640 with GlutaMAX
Choose RPMI 1640 with GlutaMAX and HEPES
Need a custom RPMI 1640 Media formulation? Customize your RPMI 1640 Media online
Find the right Gibco media formulation
Use our interactive Media Formulation tool to compare basal media side-by-side. First choose your media, and then choose specific modifications to find the right match for your experiment. Easily compare selected media with the download feature for your future reference.
View formulations
Resources
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.
Gibco Cell Culture Basics
The Gibco Cell Culture Basics handbook and videos can help your lab attain reproducible results.
Related products
Support
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support? Contact our expert team for technical and application support of Laboratory Products
