Gene Expression

Gibco Tet-System Approved FBS has been functionally tested to provide researchers with optimal control over their gene expression systems, thus minimizing challenges that can be posed by this type of reagent.

Functional, cell-based assays that detect the presence of tetracycline and its derivatives are used to select FBS that delivers the highest range of induction.

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Why cell-based testing?

  • Provides confidence, convenience, and control over your research:
  • Higher sensitivity compared to analytical methods
  • Enables sensitivity in gene expression experiments down to nanogram quantities of tetracycline
  • Detects the presence of tetracycline and tetracycline analogs (e.g., doxycycline)
  • Allows for greater control of inducible expression systems

 

Areas of research utilizing tetracycline-inducible system with FBS

Ideal for researchers studying Example
Neuroscience Produce GABAA receptors for electrophysiological study1
Cancer research Quantitatively control PVT1 expression by tet-on in bladder cancer2 Tetracycline-regulated shRNA expression to apply to monocyte interaction3
Drug screening Establish cell line with inducible mGlutamate receptor for drug screening4
Vaccine development Research for the safer vaccinia virus vaccines with tet-inducible system5
Gene editing CRISPR/Cas9-mediated control of NFAT56  and PVT1 and ANRIL7

1. Protein Science 19:1728–1738 (2010).  2. Oncotarget 6(38):41194–41203 (2015).  3. Journal of Visualized Experiments 129:e56333 (2017).  4. Journal of Biomolecular Screening 20(3):350–358 (2015).  5. Virus Research 181:43–52 (2014).  6. Journal of Animal Science and Biotechnology 10:43 (2019).  7. Oncology Letters 16:4309–4316 (2018).

Why researchers select Tet-System Approved FBS
“I require tight control of gene expression and I need to ensure genes are not expressed until tetracycline is added in, so tet-free is a requirement.”

– Cancer researcher at US biotechnology company

Did you know?

Unintended expression can occur in very sensitive tet-inducible gene expression systems while using FBS. This happens because some lots of FBS contain residual amounts of the antibiotic tetracycline (tet) derived from animal treatment. It is important to use functionally tested FBS and don’t base your decisions solely on names like Tet-Free or Tet-Zero.

Using Tet-System Approved FBS is the most secure and shortest way for inducible expression systems

Tip
Tip: Looking to save some of your research budget? You can maintain your cell cultures in standard FBS and introduce Tet-System Approved FBS in the critical days prior to running assays.

Ordering information

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.