Supporting cells in the freezer

The use of a freezing medium, which should contain a cryoprotective agent such as dimethylsulfoxide (DMSO) or glycerol, is highly recommended for cryopreserving your cells. Cryoprotective agents reduce the freezing point of the medium and allow a slower cooling rate, greatly reducing the risk of ice crystal formation that can damage cells and cause cell death.

Gibco cryopreservation media were designed to support safe cryopreservation of your cell lines for future use.

Which Gibco cryopreservation solution best fits your research needs?

 Synth-a-Freeze Cryopreservation MediumRecovery Cell Culture Freezing MediumPSC Cryopreservation Kit
Intended for:Freezing and storing a variety of cell types Cryopreservation of a wide variety of mammalian cellsCryopreservation medium and recovery supplement optimized to enable maximum pluripotent stem cell viability
Tested cell typesHuman keratinocytes, ESCs, MSCs, NSCs, other primary cell typesCHO-S, CHO-K1, HEK 293, Jurkat, NIH 3T3Pluripotent stem cells
Chemical compositionAOFContains FBSXeno-free
Ready-to-use
cGMP-manufactured
Cell Therapy Systems (CTS) product availableCTS Synth-a-Freeze Medium-CTS PSC Cryopreservation Kit
Size50 mL50 mL50 mL bottle + 5 mL vial
Cost$$$$$$
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Resources

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Stem Cell Research Learning Center
Access a collection of resources to help ensure your stem cell research success.

Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

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Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

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