Jurkat cells were treated with 0.05% trypsin (A) or TrypLE Express reagent (B) for a period of up to 20 minutes. Cell-surface CD2 levels were then measured via flow cytometry with an APC-conjugated anti-CD2 monoclonal antibody. As demonstrated, cells treated with 0.05% trypsin show a clear time-dependent reduction in CD2 levels, those treated with TrypLE Express reagent exhibit no measurable CD2 loss.
TrypLE Reagents
The superior replacement for trypsin
Gibco TrypLE reagents are highly purified, recombinant cell-dissociation enzymes that replace porcine trypsin. These reagents are ideal for dissociating attachment-dependent cell lines in both serum-containing and serum-free conditions, and can be directly substituted for trypsin without protocol changes.
TrypLE reagents are:
- Gentle on cells—protect your cell’s surface proteins
- Stable at room temperature—no need to freeze means they’re ready when you need them
- Animal origin–free—important if you’re looking for a product without animal-derived components
TrypLE Express reagent outperforms trypsin in preserving cell-surface epitope expression
Flow cytometry analysis: cell dissociation from mouse CNS tissue
Flow cytometry is an invaluable part of the scientist’s toolbox in the field of cell biology, providing a wealth of information. In one study, different cell dissociation reagents were compared and their impact on cell viability and membrane protein integrity assessed. The authors concluded that dissociation with TrypLE reagent is efficient and preserves both cell viability and cell-surface protein epitope integrity.
Cell viability is better with TrypLE reagent than with Accutase reagent. Dissociation time: 10min; Viability (%) +/- SEM. Adapted from data shown in Stem Cells, 25:1560 (2007). doi:10.1634/stemcells.2006-0260
The CD24 epitope is retained with TrypLE whereas use of papain illustrates complete loss of the CD24 epitope (Adapted from data shown in Stem Cells, 25:1560 (2007). doi:10.1634/stemcells.2006-0260).
TrypLE Express reagent outperforms trypsin in preserving cell-surface epitope expression
Jurkat cells were treated with 0.05% trypsin (A) or TrypLE Express reagent (B) for a period of up to 20 minutes. Cell-surface CD2 levels were then measured via flow cytometry with an APC-conjugated anti-CD2 monoclonal antibody. As demonstrated, cells treated with 0.05% trypsin show a clear time-dependent reduction in CD2 levels, those treated with TrypLE Express reagent exhibit no measurable CD2 loss.
Flow cytometry analysis: cell dissociation from mouse CNS tissue
Flow cytometry is an invaluable part of the scientist’s toolbox in the field of cell biology, providing a wealth of information. In one study, different cell dissociation reagents were compared and their impact on cell viability and membrane protein integrity assessed. The authors concluded that dissociation with TrypLE reagent is efficient and preserves both cell viability and cell-surface protein epitope integrity.
Cell viability is better with TrypLE reagent than with Accutase reagent. Dissociation time: 10min; Viability (%) +/- SEM. Adapted from data shown in Stem Cells, 25:1560 (2007). doi:10.1634/stemcells.2006-0260
The CD24 epitope is retained with TrypLE whereas use of papain illustrates complete loss of the CD24 epitope (Adapted from data shown in Stem Cells, 25:1560 (2007). doi:10.1634/stemcells.2006-0260).
Choose the TrypLE product that fits your needs
See TrypLE Express products, manufactured to make this trypsin replacement reagent easily accessible to all researchers
See TrypLE Select (1X) products, manufactured on dedicated animal origin-free equipment
See TrypLE Select (10X) products, fast dissociation of strongly adherent cells and manufactured on dedicated animal origin–free equipment
Gibco TrypLE Express reagent proves that superior performance can be affordable. Significant economies of scale make it even easier to replace trypsin with TrypLE Express reagent. For fast, effective cell dissociation that doesn’t harm your cells, use TrypLE Express reagent.
Gibco TrypLE Select (1X) reagent is manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York.
Cell release times with TrypLE Select (1X) reagent
| TrypLE Select Reagent | |||
|---|---|---|---|
| Cell line | Media | Mean time required for cell release (min) |
Mean viability (%) |
| 293F | DMEM + 5% FBS | 2 | 97 |
| VERO | VP-SFM | 5 | 100 |
| VERO | EMEM + 5% FBS | 8 | 100 |
| VERO | OptiPRO SFM | 7 | 98 |
| CHO-K1 | CHO III A | 7 | 95 |
Gibco TrypLE Select (10X) reagent can be used at full strength to quickly and gently dissociate strongly adherent cell types. TrypLE Select (10X) reagent is also manufactured on dedicated animal origin–free equipment in our state-of-the art facilities in Grand Island, New York.
Strongly adherent cell release times
| TrypLE Select (10X) Reagent | TrypLE Select Reagent | ||||
|---|---|---|---|---|---|
| Strongly adherent cell lines |
Media | Mean time required for cell release (min) |
Mean viability (%) |
Mean time required for cell release (min) |
Mean viability (%) |
| PK-15 | EMEM + 10% FBS | 11.7 ± 2.0 | 99.4 ± 0.3 | 27 | 98 |
| PK-15 | Gibco OptiPRO SFM | N/A | N/A | 11 | 99 |
| MDCK | OptiPRO SFM | 15.3 ± 0.8 | 98.7 ± 0.5 | 28 | 98 |
| MDBK | DMEM + 5% FBS | 8.0 ± 1.3 | 98.8 ± 0.5 | 15 | 100 |
| A549 | DMEM + 10% FBS | 5.2 ± 0.3 | 99.1 ± 0.5 | 9 | 98 |
| MSC | DMEM + 10% MSC FBS | 7.8 ± 1.2 | 98.7 ± 1.3 | N/A | N/A |
| MSC | Gibco StemPro MSC SFM | 8.7 ± 1.5 | 97.5 ± 0.1 | N/A | N/A |
Resources
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