Growth factors are the unique cell signaling molecules that play important roles in cell proliferation and development. It's a fact that cell cultures can greatly benefit from the use of pure, high quality proteins.
Fibroblast Growth Factor basic, bFGF, FGF basic or FGF 2, belongs to a large FGF protein family involved in many aspects of development including cell proliferation, growth, and differentiation. FGF-basic is a critical component for maintaining embryonic stem cells in culture in an undifferentiated state.
EGF, Epidermal Growth Factor, acts a potent mitogenic factor that plays an important role in the growth, proliferation and various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Dysregulation of EGF production has been associated with the growth and progression of certain cancers.
Brain-Derived Neurotrophic Factor (BDNF) is a used in the maintenance of motor neurons and the differentiation of both cholinergic and dopaminergic neurons.
Human Interleukin 2 (IL2) causes proliferation of T-cells and is a central regulator of immune responses. IL2 stimulates the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes.
Vascular Endothelial Cell Growth Factor (VEGF), is a part of the platelet-derived family of growth factors. VEGF is involved in endothelial cell proliferation, angiogenesis and vasculogenesis. VEGF has also been shown to be chemotactic for monocytes and osteoblasts.
Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) is involved in many biological responses including the growth and development of granulocyte and macrophage progenitor cells, stimulation and the initiation of differentiation of myeloblasts and monoblasts, and chemotaxis of eosinophils.
For those who seek a consistent supply of recombinant cytokines throughout the drug discovery and development journey, Gibco PeproTech cytokines and growth factors are manufactured solely in-house to maintain quality and provide high compatibility across the RUO-to-GMP spectrum.
Figure 2. Structural homogeneity. The SDS-PAGE gel was run on Human BMP-2 (Cat. No. PHC7145) under reducing (lanes 2 and 3) and non-reducing (lanes 4 and 5) conditions. Under non-reducing conditions, BMP-2 is seen as a dimer, consistent with biologically active BMP-2.
Figure 3. More bioactive protein due to exceptional refolding techniques. Proliferation of MO7e cells in response to SCF from Gibco and the competitor (Cat. No. PHC2115). As illustrated by the lower ED50, less Gibco SCF is required to yield a response.
Figure 4. Protease activity confirms protein functionality. Enzymatic activity of Kallikrein 4 (KLK4) was measured by its ability to cleave fluorogenic peptide substrate Boc-Val-Pro-Arg-AMC. The end product is measured using fluorescence excitation and emission wavelengths of 380 nm and 460 nm, respectively.
Figure 6. Calcium flux confirms expected biological response. Calcium flux activation was observed by stimulating Fura-2–loaded rat PMNs with the Gibco chemokine CINC-2β.
To help you get your desired cell culture results every time, we’ve compiled a list from experienced researchers of important facts about growth factors.
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