• First commercially available, fully defined serum-free media for immunological studies
  • The only FDA 510(k) cleared commercial T cell medium for human ex vivo tissue and cell culture processing applications
  • Lot-to-lot consistency and produced under cGMP

Superior performance, no serum required

Gibco AIM V Medium does not require human serum for optimal growth and activation of differentiated lymphoid cells.

  • Minimizes risk of viral immunogenicity associated with human serum
  • Contains no added cytokines and lymphokines
  • All proteins in the formulation have the natural human sequence

Taking your work to the clinic? Be sure to check out CTS AIM V Medium

AIM V versus human serum–supplemented medium

Figure 1 compares relative LAK cell lytic activity of lymphocytes maintained in AIM V Medium with lymphocytes maintained in RPMI-1640 supplemented with serum.

       Figure 1. Comparison of LAK cell lytic activity: AIM V Medium versus human serum–supplemented medium. Human peripheral blood lymphocytes (PBLs) were cultured in RPMI-1640 with 1,000 units/mL of IL-2 plus 2% human serum type AB. Human PBLs were also cultured in serum-free AIM V Medium with 600 units/mL of IL-2. Results are expressed as averages of duplicate flasks assayed in triplicate using Daudi cells as targets.

Applications of AIM V Medium

AIM V Medium has been tested for broad range of applications in adoptive immunotherapy and cellular immunology research. See some of the applications here:

LAK cell induction & function Cytokine & antibody effects on LAK cell induction
Immunotherapy of murine hepatic metastases Lymphocyte receptor studies
TIL cell generation in hollow fiber bioreactors NK cells & NK-like effector populations
T cell activation In vitro & in vivo model systems for adoptive immunotherapy
Cytokine regulation of effector cells HIV research
Cytokine receptor & postreceptor events in lymphoid effector cells Macrophage activation
Hybridoma growth & monoclonal antibody production Antibody-dependent cellular cytotoxicity
Effector cells in antitumor immunity Long-term cultivation of functional human macrophages
In vitro studies of lymphokines & antibodies Mechanisms of cell-mediated cytotoxicity

Cells types grown using AIM V Medium

T cell types

  • CD8+ T lymphocytes
  • Polyclonal T cells
  • Peripheral blood lymphocytes (PBL)
  • CD4 cells for HIV patients
  • Tumor infiltrating lymphocytes (TIL)
  • Retroviral transduced PBL
  • CD3 positive NK T cells
  • Antigen-specific cells (not clonal)
  • γδ T cells
  • NK cells

Other cell types

  • Macrophages
  • Human monocytes
  • HUT78 (human)
  • Dendritic cells
  • HL-60
  • COS cells (monkey)
  • YAC-1 (mouse)
  • Murine lymphoma
  • Myeloid cells
  • Leukemic cells
  • Human fibroblasts
  • VERO
  • Oral epithelial

About AIM V Medium