- First commercially available, fully defined serum-free media for immunological studies
- Lot-to-lot consistency and produced under cGMP
Superior performance, no serum required
Gibco AIM V Medium does not require human serum for optimal growth and activation of differentiated lymphoid cells.
- Minimizes risk of viral immunogenicity associated with human serum
- Contains no added cytokines and lymphokines
- All proteins in the formulation have the natural human sequence
AIM V versus human serum–supplemented medium
Figure 1 compares relative LAK cell lytic activity of lymphocytes maintained in AIM V Medium with lymphocytes maintained in RPMI-1640 supplemented with serum.
|Figure 1. Comparison of LAK cell lytic activity: AIM V Medium versus human serum–supplemented medium. Human peripheral blood lymphocytes (PBLs) were cultured in RPMI-1640 with 1,000 units/mL of IL-2 plus 2% human serum type AB. Human PBLs were also cultured in serum-free AIM V Medium with 600 units/mL of IL-2. Results are expressed as averages of duplicate flasks assayed in triplicate using Daudi cells as targets.|
Applications of AIM V Medium
AIM V Medium has been tested for broad range of applications in adoptive immunotherapy and cellular immunology research. See some of the applications here:
|LAK cell induction & function||Cytokine & antibody effects on LAK cell induction|
|Immunotherapy of murine hepatic metastases||Lymphocyte receptor studies|
|TIL cell generation in hollow fiber bioreactors||NK cells & NK-like effector populations|
|T cell activation||In vitro & in vivo model systems for adoptive immunotherapy|
|Cytokine regulation of effector cells||HIV research|
|Cytokine receptor & postreceptor events in lymphoid effector cells||Macrophage activation|
|Hybridoma growth & monoclonal antibody production||Antibody-dependent cellular cytotoxicity|
|Effector cells in antitumor immunity||Long-term cultivation of functional human macrophages|
|In vitro studies of lymphokines & antibodies||Mechanisms of cell-mediated cytotoxicity|
Cells types grown using AIM V Medium
Other cell types
- Human monocytes
- HUT78 (human)
- Dendritic cells
- COS cells (monkey)
- YAC-1 (mouse)
- Murine lymphoma
- Myeloid cells
- Leukemic cells
- Human fibroblasts
- Oral epithelial
- Dietze B., et al. (2008) An improved method to generate equine dendritic cells from peripheral blood mononuclear cells: divergent maturation programs by IL-4 and LPS. Immunobiology 213:751–758.
- Meehan K.R., et al. (2008) Development of a clinical model for ex vivo expansion of multiple populations of effector cells for adoptive cellular therapy. Cytotherapy 10: 30–37.
- Ye Z., et al. (2006) Human dendritic cells engineered to express alpha tumor necrosis factor maintain cellular maturation and T-cell stimulation capacity. Cancer Biother Radiopharm 21:613–622.
- Choi B.H., et al. (2006) Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemic cells for clinical immunotherapy. J Clin Apher 21: 233–240.
- Imataki O., et al. (2006) Efficient ex vivo expansion of Valpha24+ NKT cells derived from G-CSF-mobilized blood cells. J Immunother 29: 320–327.
- Peng J.C., et al. (2005) Generation and maturation of dendritic cells for clinical application under serum-free conditions. J Immunother 28: 599–609.
- Trickett A.E., et al. (2002) Ex vivo expansion of functional T lymphocytes from HIV-infected individuals. J Immunol Methods 262: 71–83.
- Carlens S., et al. (2000) Ex vivo T lymphocyte expansion for retroviral transduction: influence of serum-free media on variations in cell expansion rates and lymphocyte subset distribution. Exp Hematol 28: 1137–1146.
- Kambe N et al. (2000) An improved procedure for the development of human mast cells from dispersed fetal liver cells in serum-free culture medium. J Immunol Methods 240: 101–110.
- Gerin P.A., et al. (1999) Production of retroviral vectors for gene therapy with the human packaging cell line FLYRD18. Biotechnol Prog 15: 941–948.
- Slunt J.B., et al. (1997) Human T-cell responses to Trichophyton tonsurans: inhibition using the serum free medium Aim V. Clin Exp Allergy 27: 1184–1192.
- Kreuzfelder E., (1996) Assessment of peripheral blood mononuclear cell proliferation by [2-3H]adenine uptake in the woodchuck model. Clin Immunol Immunopathol 78: 223–227.
- Causey A.L., (1994) A serum-free medium for human primary T lymphocyte culture. J Immunol Methods 175: 115–121.
- Freedman R.S., et al. (1994) Large-scale expansion in interleukin-2 of tumor-infiltrating lymphocytes from patients with ovarian carcinoma for adoptive immunotherapy. J Immunol Methods 167: 145–160.
- Nomura K., et al. (1993) [Study of adoptive immunotherapy for metastatic renal cell carcinoma with lymphokine-activated killer (LAK) cells and interleukin-2. II. Clinical evaluation] Nippon Hinyokika Gakkai Zasshi 84: 831–840. Japanese.
- Kaldjian E.P., et al. (1992) Enhancement of lymphocyte proliferation assays by use of serum-free medium. J Immunol Methods 147: 189–195.
- Hayakawa K., et al. (1991) Study of tumor-infiltrating lymphocytes for adoptive therapy of renal cell carcinoma (RCC) and metastatic melanoma: sequential proliferation of cytotoxic natural killer and noncytotoxic T cells in RCC. J Immunother 10: 313–325.
- McVicar D.W., et al. (1991) A comparison of serum-free media for the support of in vitro mitogen-induced blastogenic expansion of cytolytic lymphocytes. Cytotechnology 6: 105–113.
- Burg S., et al. (1991) [Effect of different media on long-term cultivation of human synovial macrophages] Z Rheumatol 50: 142–150. German.
- Helinski E.H., et al. (1988) Long-term cultivation of functional human macrophages in Teflon dishes with serum-free media. J Leukoc Biol 44: 111–121.
- Robyn S., et al. (2007) RA8, A Human Anti-CD25 Antibody Against Human Treg Cells Hybridoma 26: 119–130.
- Chena X., et al.(2006) Induction of primary anti-HIV CD4 and CD8 T cell responses by dendritic cells transduced with self-inactivating lentiviral vectors Cellular Immunology 243: 10–18.
- Grant R., et al. (2008) CCL2 Increases X4-tropic HIV-1 Entry into Resting CD4+ T Cells. J Biol Chem 283: 30745–30753.
- Hagihara M., et al. (2003) Increased frequency of CD3/8/56-positive umbilical cord blood T lymphocytes after allo-priming in vitro Annals of Hematology 82: 166–170.
- Wang, Z., et al. (2006) Application of Serum-Free Culture Medium for Preparation of A-NK Cells Cell Mol Immunol 3: 391–395.
- Morecki S., et al. (1991) Retrovirus-mediated gene transfer into CD4+ and CD8+ human T cell subsets derived from tumor-infiltrating lymphocytes and peripheral blood mononuclear cells Cancer Immunol Immunotherapy 32: 342–352.
- Johansen P. et al. Cellular immune response—CD4 T cells guarantee optimal competitive fitness of CD8 memory T cells Eur J Immunol 34: 91–97.
Additional AIM-V medium references
- Rebecca J et al., (2010) Natural exposure to cutaneous anthrax gives long lasting T cell immunity encompassing infection-specific Epitopes. J. Immunol., 2010; 184: 3814 – 3821
- Fabricius D et al., (2010) Prostaglandin E2 inhibits IFN-α secretion and Th1 costimulation by human plasmacytoid dendritic cells via E-prostanoid 2 and E-prostanoid 4 receptor engagement. J. Immunol., 2010; 184: 677 - 684.
- Jahrsdorfer B et al., Granzyme B produced by human plasmacytoid dendritic cells suppresses T-cell expansion. Blood, 2010; 115: 1156 - 1165.
- Tam V et al., The RgpA-Kgp Proteinase-Adhesin Complexes of Porphyromonas gingivalis Inactivate the Th2 Cytokines Interleukin-4 and Interleukin-5. Infect. Immun., 2009; 77: 1451 - 1458.
- Ando T et al., Transduction with the Antioxidant Enzyme Catalase Protects Human T Cells against Oxidative Stress J. Immunol., 2008; 181: 8382 - 8390.
- Nesbit L et al., Polyfunctional T Lymphocytes Are in the Peripheral Blood of Donors Naturally Immune to Coccidioidomycosis and Are Not Induced by Dendritic Cells. Infect. Immun., 2010; 78: 309 - 315.
- Csillag A et al., Pollen-Induced Oxidative Stress Influences Both Innate and Adaptive Immune Responses via Altering Dendritic Cell Functions. J. Immunol., 2010; 184: 2377 - 2385.
- Bellone S et al., Human Papillomavirus Type 16 (HPV-16) Virus-Like Particle L1-Specific CD8+ Cytotoxic T Lymphocytes (CTLs) Are Equally Effective as E7-Specific CD8+ CTLs in Killing Autologous HPV-16-Positive Tumor Cells in Cervical Cancer Patients: Implications for L1 Dendritic Cell-Based Therapeutic Vaccines. J. Virol., 2009; 83: 6779 - 6789.
- Liu ZW et al., A CD26-Controlled Cell Surface Cascade for Regulation of T Cell Motility and Chemokine Signals. J. Immunol., 2009; 183: 3616 - 3624.
- Megyeri M et al., Complement Protease MASP-1 Activates Human Endothelial Cells: PAR4 Activation Is a Link between Complement and Endothelial Function. J. Immunol., 2009; 183: 3409 - 3416.
- Asish K. et al., Curcumin Inhibits Prosurvival Pathways in Chronic Lymphocytic Leukemia B Cells and May Overcome Their Stromal Protection in Combination with EGCG. Clin. Cancer Res., 2009; 15: 1250 -1258
- Cornberg M et al., CD8 T Cell Cross-Reactivity Networks Mediate Heterologous Immunity in Human EBV and Murine Vaccinia Virus Infections. J. Immunol., 2010; 184: 2825 - 2838.
- Ariadne L et al., The Gli3 Transcription Factor Expressed in the Thymus Stroma Controls Thymocyte Negative Selection Via Hedgehog-Dependent and -Independent Mechanisms. J. Immunol., 2009; 183: 3023 - 3032.
- Hagn M et al., Human B Cells Secrete Granzyme B When Recognizing Viral Antigens in the Context of the Acute Phase Cytokine IL-21. J. Immunol., 2009; 183: 1838 - 1845.
- Lenka L et al., Apolipoprotein-mediated lipid antigen presentation in B cells provides a pathway for innate help by NKT cells. Blood, 2009; 114: 2411 - 2416.
- Toll-like Receptor-7 Tolerizes Malignant B Cells and Enhances Killing by Cytotoxic Agents. Cancer Res., 2007; 67: 1823 - 1831.
- Andrei V et al., Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor B target genes and induction of apoptosis in chronic lymphocytic leukemia B cells. Blood, 2007; 110: 735 - 742.
- Caitlin M et al., Extracellular calcium sensing promotes human B-cell activation and function. Blood, 2007; 110: 3985 - 3995.
AIM V medium is widely used on T cells, T cell lines, lymph node cells and dendritic cells.
- HUVEC cells is reported to culture in AIM V with 1% FBS and growth factors (reference 10)
- CLL B cell (reference 11, 17)
- CLL cells (reference 16)
- Fetal thymus (reference 13)
- Human B cells (reference 14, 15, 18)