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Thermo Fisher Scientific offers cryopreserved primary astrocytes for your neuroscience research. When thawed and cultured in vitro, these neural cell cultures exhibit astrocytic phenotypic markers and high viability.
Astrocytes are large, star-shaped neural cells found in the brain. Astrocytes are the most common cells in the central nervous system and comprise several sub-types based on location within the CNS. They perform a variety of functions including neurotransmitter regulation, support of neuronal health and development as well as immune cell function and metabolism.
Primary astrocytes are harvested from tissue and have not been cultured extensively. Unlike neurons, primary astrocytes can be cultured and expanded in vitro.
Cultured astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.
A. Gibco Rat Primary Cortical Astrocytes are isolated from the cortex of fetal (E19) Sprague Dawley rats.
A. Gibco Rat Primary Cortical Astrocytes were cryopreserved in growth medium containing 10% DMSO at passage 1 (P1).
A. There are few known differences between cortical and hippocampal astrocytes. However, it has been reported that astrocytes from different regions of the brain show a differential sensitivity to ischemic injury (Zhao and Flavin, 2000; Xu et al., 2001).
A. Primary rat astrocytes are typically isolated from late fetal or early postnatal brain because the resulting astrocytes are more viable in culture and of higher purity than astrocytes from adult brain.
A. Upon thawing, the vial has more than 70% viability, which will give more than one million live cells.
A. Cell purity was evaluated in terms of the expression of astrocyte specific marker, GFAP. More than 80% of the cells were positive for GFAP marker and the expression of neuronal marker (DCX) and oligodendrocyte marker (GalC) were less than 10%.
A. Gibco Rat Primary Cortical Astrocytes can be thawed, recovered, and proliferated up to three passages to yield 1.5–2 fold increase of cells.
A. The doubling time of Gibco Rat Primary Cortical Astrocytes is about 8.7 days.
A. Clean the coverslips and then coat them with 0.01% poly-D-lysine in double distilled water.
A. Gibco Rat Primary Cortical Astrocytes were dissociated into single cells before cryopreservation, which gives an advantage to control the number of cells to plate.
A. Astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.
Cell type | Size | Cat. No. | Medium |
---|---|---|---|
Rat cortical astrocytes | 1 x 106 cells | N7745-100 | 85% DMEM (high glucose) and 15% Fetal Bovine Serum (FBS) |
For Research Use Only. Not for use in diagnostic procedures.