Rat Cortex and Hippocampus Neuronal Cells
|Gibco® Primary Rat Cortex and Hippocampus Neurons are isolated from day-18 Fisher 344 rat embryos and cryopreserved in liquid nitrogen. These cells offer neuroscience researchers a flexible, ready-to-use, high-quality alternative to freshly isolated neurons.|
Rat cortex and hippocampus neuronal cells
These products are developed through vigorous internal research to afford comparable properties, such as viability, purity, and cell function, but minimize the inherent lot-to-lot variability with cells that are acutely isolated. Unlike the other commercially available primary rat neurons that generally yield about 30% or lower post-thaw viability, Gibco® neuron cells consistently give post-thaw viability in the range of 50–80% and cells grown in Neurobasal® medium plus B-27® supplements show minimum or absence of glial cell growth.
Figure 1. Comparison of post thaw viability of primary rat cortex neurons with competitors' products.
Figure 2. Comparison of post thaw viability of primary hippocampus neurons with competitors' products.
Figure 3. Primary Rat Hippocampus neurons. Immunoflorescence detection of primary neuronal cells stained with mouse anti-MAP2 antibody (green) and singular presence of astrocyte as detected by rabbit anti-GFAP marker (red). Nuclei are stained with DAPI (blue).
Long-term viability of neurons using the LIVE/DEAD® Viability/Cytotoxicity assay
|1 Day||14 Days||22 days|
|Figure 4. Long-term viability of neurons using the LIVE/DEAD® Viability/Cytotoxicity assay. Rat cortical neurons cultured in Neurobasal® medium supplemented with B-27® supplement and GlutaMAX™-I. Live cells are stained with calcein-AM (green) and dead cells are stained with EthD-1 (red); (LIVE/DEAD® Viability/Cytotoxicity Kit for mammalian cells).|
For Research Use Only. Not for use in diagnostic procedures.