Transfection is the process by which nucleic acids are introduced into mammalian cells. Protocols and techniques vary widely and include lipid transfection and chemical and physical methods such as electroporation. Expertise in cellular and molecular biology is behind the Invitrogen product portfolio of DNA reagents and consumables, which are the most cited and trusted transfection and selection systems available. Use this overview of our most effective transfection products to choose the solution that’s right for you

See the Transfection Reagent Selection Guide

Featured application note: Versatility and performance of Lipofectamine Stem Transfection Reagent

The goal of this study was to illustrate the versatility of Invitrogen Lipofectamine Stem Transfection Reagent, which was developed specifically for stem cells, to co-deliver multiple payloads in addition to large plasmid constructs with high transfection efficiency. Lipofectamine Stem reagent can be used to transfect a wide range of stem cell types with superior efficiency while supporting continued proliferation without inducing differentiation.

 Download the application note

Transfection Products

By sample type

By cell type

Our protocols for Lipofectamine Reagent have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially with a new cell line.

If you are working with cancer cell lines, we’ve developed and validated cancer cell line-specific protocols covering the most researched cell lines in breast cancer, lung cancer, and liver cancer.

If neither of these options is working for your specific cell type, consult our additional cell type–specific protocols for further optimization.

Transfection Reagents

Designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures. Lister below are Invitrogen product lines:

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Transfection Methods

Resources

For Research Use Only. Not for use in diagnostic procedures.