Transfection

Transfection is the process by which nucleic acids are introduced into eukaryotic cells. Protocols and techniques vary widely and include lipid transfection and chemical and physical methods such as electroporation. Expertise in cellular and molecular biology is behind the Invitrogen product portfolio, which are the most cited and trusted transfection and selection systems available.

Use this overview of our most effective transfection products to choose the solution that’s right for you.

Transfection reagent selection tool

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 Download the application note

By sample type

• Plasmid DNA transfection
• siRNA transfection
• in vivo transfection
• mRNA

By cell type

Our protocols for Lipofectamine Reagent have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially with a new cell line.

If you are working with cancer cell lines, we’ve developed and validated cancer cell line-specific protocols covering the most researched cell lines in breast cancer, lung cancer, and liver cancer.

If neither of these options is working for your specific cell type, consult our additional cell type–specific protocols for further optimization.

Designed for effective and efficient nucleic acid delivery into both traditional and difficult-to-transfect cell lines and for transient transfection in suspension cultures. Lister below are Invitrogen product lines:

• Lipofectamine 3000
• Lipofectamine Stem
• Lipofectamine CRISPRMAX
• Invivofectamine 3.0
• Lipofectamine MessengerMAX
• Lipofectamine RNAiMAX
• Lipofectamine 2000

Compare Lipofectamine reagents › 
View all transfection reagents ›

• Lipid transfection
• Electroporation
• Transient transfection
• CRISPR transfection
• In vivo transfection
• Cotransfection
• Stable transfection
• RNAi and siRNA transfection
• Calcium phosphate transfection
• Large scale protein production