The Neon Transfection System offers an innovative electroporation method that utilizes a proprietary biologically compatible pipette tip chamber to generate a more uniform electric field for a significant increase in transfection efficiency and cell viability.

Get improved transfection outcomes with the Neon Transfection System

  • Efficiency—up to 90% transfection and gene-editing efficiency in extremely difficult cells, including immune, primary, and stem cells; over 140 cell lines were tested with optimized ready-to-use conditions for efficiency and viability
  • Flexibility—open system allows electroporation parameters to be optimized freely; easily transfect from 2 x 104 cells to 6 x 106 cells per reaction
  • Simplicity—single reagent kit for all cell types
  • Versatility—can be used to deliver DNA, RNA, and protein


Ordering information for Neon Transfection System


Achieve high transfection efficiency and high cell viability in a broad range of cell lines with Neon Electroporation System

The Neon Transfection System is an electroporation instrument that offers up to 90% transfection and gene-editing efficiency in extremely difficult-to-transfect cells, including immune, primary, and stem cells. Over 140 cell lines have been tested with optimized ready-to-use conditions for efficiency and viability.

Search the Transfection protocols and citations database for information on cell lines successfully transfected using the Neon Electroporation System.

Panel A displaying the high transfection efficiency of Jukrat cells with the Neon Transfection System via electron microscopy and panel B is via fluorescence imaging.

Figure 1. High transfection efficiency of Jurkat cells with Neon Transfection System. Intracellular uptake of reporter vector encoded with EGFP at 24 hr following transfection of Jurkat cells with Neon Transfection System. B is the corresponding fluorescence image of A.



How to use a Neon Transfection System

Plug-n-play out of the box

The transfection process is simple: take up the cells and plasmid mix into the Neon pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon system uses a simple three-step transfection procedure. The transfection occurs in the Neon pipette tip.

Infographic showing the three steps of using the Neon Electroporation system, with step 1 showing loading of the cells into the pipette tip, step 2 showing selection of the protocol on the monitor, and step 3 showing delivery of the transfected cells into a culture vessel
  1. Load mix of harvested cells and molecules to be delivered (e.g., DNA, RNA, protein) into the Neon pipette tip.
  2. Plug the pipette into position in the Neon transfection device; select your protocol, and press Start.
  3. Unplug the pipette and transfer your transfected cells into a tissue culture vessel.

Simplified transfection kit for all cell types

Avoid the hassle of determining which proprietary buffer will work with your favorite cell type. Simplify your workflow—Neon electroporation kits contain all the buffers and consumables you need and are compatible with all cell types. Start your transfection with our optimized protocols for popular cell types or follow our standard  simple optimization procedure for all new cell types.


A step-by-step video on how to use the Neon Electroporation System



Specialized Neon pipette tip: a better alternative to electroporation cuvettes

Unlike standard cuvette-based electroporation chambers, the Neon electroporation system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows a better maintenance of physiological conditions resulting in high cell survival compared to conventional electroporation.*

Standard electroporation cuvette is pictured on the left and the Neon electroporation tip is on the right.

Figure 2. Standard electroporation cuvette compared to Neon pipette tip. The design of the electrode in pipette has been shown to produce a more uniform electric field. The result is less toxicity to the cells and higher transfection efficiencies.

Resources

Transfection protocols and citations

Search this library to find protocols and citations curated to fit your experimental needs. Filter by cell type, payload, and product of choice to easily unlock your answers.

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Cell Culture and Transfection Learning Center
Access cell culture and transfection application resources for more success as you plan and execute your experiments.

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Transfection FAQs

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For Research Use Only. Not for use in diagnostic procedures.

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