Neon NxT Electroporation System:
Trusted Transfection Performance

Protect your valuable research time and samples with a compact electroporation machine designed and engineered for performance, speed, simplicity, and flexibility. The combination of the Invitrogen Neon NxT Electroporation System’s proprietary pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers contamination risk, saves you steps, and can put you in control of your experiments.

Benefits of the Neon NxT Electroporation System

• Proven transfection efficiency and cell viability—transfect challenging cell lines with high efficiency and high cell viability
• Flexibility when you need it—precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
• Enhanced genome editing efficiency—optimized buffer for improved delivery of CRISPR molecules
• Save valuable research time—transfect cells quickly with an easy three step workflow, a single buffer kit and intuitive user interface
• Preserve your precious samples—minimize sample transfer loss and contamination risk

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The Neon NxT Electroporation System is an electroporation instrument that offers up to 90% transfection and gene-editing efficiency in extremely difficult-to-transfect cells, including immune, primary, and stem cells. Over 150 cell lines have been tested with optimized ready-to-use conditions for efficiency and viability.

Search the Transfection protocols and citations database for optimized electroporation protocols on different cell lines.

Genome editing holds great promise in the pursuit of a greater understanding of human health and disease. A critical, yet challenging step in the genome editing workflow is the effective delivery of CRISPR ribonucleoprotein (RNP), DNA and RNA molecules into the cell line of choice—also known as transfection. As demonstrated above, electroporation is the most widely used delivery method for its ability to achieve high transfection efficiency, even with hard-to-transfect cells.

When used with the Neon NxT Electroporation System, our gene editing reagents and the Neon NxT Resuspension Genome Editing Buffer can help improve gene editing performance with specific payloads like CRISPR Cas9 for knockout or knock-in experiments with mammalian cells including primary, stem, and difficult-to-transfect cells (Figure 3).

Explore our comprehensive suite of gene editing workflow solutions at thermofisher.com/geneediting

Customizable electroporation parameters

With the Neon NxT Electroporation System, you can precisely control the parameters that matter most for your experiment without wasting precious time adjusting those that do not. This transfection system allows you to modify:

• Pulse voltage
• Pulse width
• Number of pulses
• Cell type
• Buffer type
• Payload type

Versatility for different payloads, cell types, and cell densities

Deliver DNA, RNA, or protein to a wide variety of mammalian cells, with the flexibility to transfect 1 x 10⁴ to 1 x 10⁷ cells per reaction.

Plan with ease using the TransfectionLab cloud-based app

Invitrogen TransfectionLab, a cloud-based app found on Thermo Fisher Connect, can automatically generate step-by-step guide tailored for each electroporation experiment, starting from the design stage. You can remotely set up multiple plate layouts for up to 384 samples, and any protocol or plate layout saved in the app can be accessed directly on the connected Neon NxT system.

Simplified electroporation workflow

Easy to follow and execute, the Neon NxT Electroporation System streamlined workflow requires minimal training and improves consistency and reproducibility.

Using this transfection system, the electroporation process is simple: first, take up cells and plasmid mix into the Neon NxT pipette tip. Second, plug into the pipette holder, and press Electroporate. Finally, pipette the transfected cells back into your culture vessel. As the transfection occurs in the pipette tip, the transfection is completed in just 3 simple steps: Aspirate, Electroporate, Dispense.

Shorter processing time using this electroporation system

In addition to simplifying the workflow, our proprietary electroporation technology shortens the overall process to 10–15 minutes, compared to a conventional electroporation system as the transfection occurs in the Neon NxT pipette tip.†

A single buffer kit for many mammalian cell types

Avoid the hassle of searching for a buffer kit that will work with your cell line. We have simplified the process with one buffer kit that is compatible with over 150 mammalian cell lines. We recommend using Invitrogen Neon NxT Resuspension R Buffer at voltages below 1,900 V and Invitrogen Neon NxT Resuspension T Buffer at higher voltages.

*The buffer tube of the Neon NxT Electroporation System has a 2 mL level indicator.
**The Neon NxT pulse generator can be moved into or out of a typical BSC without removing the sash.
***Excess cable length can be secured behind the Neon NxT system with the attachable cable organizer.

The same technology and performance as the Neon Transfection System

† Kim JA, Cho K, Shin MS, et al. (2008) A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 23(9):1353–1360.