Protect your valuable research time and samples with a compact electroporation machine designed and engineered for performance, speed, simplicity, and flexibility. The combination of the Invitrogen Neon NxT Electroporation System’s proprietary pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers contamination risk, saves you steps, and can put you in control of your experiments.

Benefits of the Neon NxT Electroporation System

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Order the Neon NxT Electroporation System

Invitrogen TransfectionLab, a cloud-based app can automatically generate step-by-step guide tailored for each electroporation experiment, starting from the design stage. You can remotely set up multiple plate layouts for up to 384 samples, and any protocol or plate layout saved in the app can be accessed directly on the connected Neon NxT system.


How-to & FAQ videos


Customer story

Neon NxT University Lab Partners Testimonial Video

In this video, Samantha Renusch, the Education Program Manager at University Lab Partners, shares her experience using the Neon NxT Electroporation System.


Achieve high transfection efficiency and high cell viability in a broad range of cell lines using this transfection system

The Neon NxT Electroporation System is an electroporation instrument that offers up to 90% transfection and gene-editing efficiency in extremely difficult-to-transfect cells, including immune, primary, and stem cells. Over 150 cell lines have been tested with optimized ready-to-use conditions for efficiency and viability.

Search the Transfection protocols and citations database for optimized electroporation protocols on different cell lines.

Graphed results from transfection efficiency and cell viability study

Figure 1. Exceptional transfection efficiency and post-transfection cell viability across diverse cell types are obtained with the Neon NxT Electroporation System. Cells were transfected with GFP plasmid DNA in 10 μL or 100 μL electroporation reactions. Transfection efficiency is reported as the percentage of GFP-positive cells (n = 3). Transfected cells were stained with Invitrogen SYTOX Red Dead Cell Stain and assessed for viability on the Invitrogen Attune NxT Flow Cytometer. Cell viability is reported as the mean of 3 measurements.


A single buffer kit for many mammalian cell type

Avoid the hassle of searching for a buffer kit that will work with your cell line. We have simplified the process with one buffer kit that is compatible with over 150 mammalian cell lines. We recommend using Invitrogen Neon NxT Resuspension R Buffer at voltages below 1,900 V and Invitrogen Neon NxT Resuspension T Buffer at higher voltages.

Enhancing genome editing efficiency

Genome editing holds great promise in the pursuit of a greater understanding of human health and disease. A critical, yet challenging step in the genome editing workflow is the effective delivery of CRISPR ribonucleoprotein (RNP), DNA and RNA molecules into the cell line of choice—also known as transfection. As demonstrated above, electroporation is the most widely used delivery method for its ability to achieve high transfection efficiency, even with hard-to-transfect cells.

When used with the Neon NxT Electroporation System, our gene editing reagents and the Neon NxT Resuspension Genome Editing Buffer can help improve gene editing performance with specific payloads like CRISPR Cas9 for knockout or knock-in experiments with mammalian cells including primary, stem, and difficult-to-transfect cells (Figure 2).

Explore our comprehensive suite of gene editing workflow solutions at thermofisher.com/geneediting

Graphed results from CRISPR knock-out/knock-in study

Figure 2. Performance of Neon NxT Resuspension Genome Editing Buffer in CRISPR-Cas9–based genome editing experiments across diverse cell types and targets. The target loci included ACTN for Jurkat and K562 cells, TRAC for activated primary T cells, B2M for HSCs, and AAVS1 for primary NK cells. Cells were electroporated in 10 μL or 100 μL reactions. (A) GFP donor DNA knock-in efficiency reported as the percentage of GFP-positive cells. (B) Viability of cells after GFP donor DNA knock-in. (C) Knockout efficiency reported as the percent reduction of a specified target locus compared to untreated control. (D) Post-electroporation viability of knockout cells.
* For primary NK cells, indel efficiency (%), determined through a genomic cleavage detection (GCD) assay, served as a proxy for knockout efficiency.



Why is the Neon NxT Electroporation System a better alternative to the electroporation cuvette?

comparison photos

Figure 3. Neon NxT pipette tip advantages compared to standard electroporation cuvette. The proprietary biologically compatible (A) Neon NxT tip presents a proven capillary electroporation technology that has important advantages over (B) electroporation cuvettes.

Unlike standard cuvette-based electroporation chambers, the Neon NxT electroporation system uses a proprietary biologically compatible pipette tip chamber that generates a more uniform electric field. This design allows for a better maintenance of physiological conditions resulting in high cell survival compared to conventional electroporation.†

  • Creates a more uniform electric field
  • Maintains a stable pH throughout the electroporation chamber
  • Generates minimal heat
  • Forms fewer ions
  • Reduces shear force experienced by cells


Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols

Customizable electroporation parameters

With the Neon NxT Electroporation System, you can precisely control the parameters that matter most for your experiment without wasting precious time adjusting those that do not. This transfection system allows you to modify:

  • Pulse voltage
  • Pulse width
  • Number of pulses
  • Cell type
  • Buffer type
  • Payload type

Versatility for different payloads, cell types, and cell densities

Deliver DNA, RNA, or protein to a wide variety of mammalian cells, with the flexibility to transfect 1 x 10to 1 x 107 cells per reaction.


Quick 3-step cell transfection with 1 or 8-channel pipette, 96 reactions in under 15 minutes, and intuitive user interface with plate mode

Simplified electroporation workflow

Easy to follow and execute, the Neon NxT Electroporation System streamlined workflow requires minimal training and improves consistency and reproducibility.

Using this transfection system, the electroporation process is simple: first, take up cells and plasmid mix into the Neon NxT pipette tip. Second, dock the pipette into the pipette station, and press Electroporate. Finally, pipette the transfected cells back into your culture vessel. As the transfection occurs in the pipette tip, the transfection is completed in just 3 simple steps: Aspirate, Electroporate, Dispense. In addition to simplifying the workflow, our proprietary electroporation technology shortens the overall process, compared to a conventional electroporation system as the transfection occurs in the Neon NxT pipette tip and therefore significantly reduces the number of pipetting steps.

8-channel pipette, greater throughput maintaining performance

With the Neon NxT 8-Channel Pipette, we elevate flexibility to an unprecedented level. Each tip of the 8-channel pipette operates independently within its own isolated tube, enabling the use of different electroporation parameters, resuspension buffer, delivery payloads, or even cell types for each tip. Additionally, the Neon NxT 8-Channel Pipette and Station works with the existing Neon NxT device as well as leverages the same Neon NxT tips and buffers. Whether you are optimizing conditions or exploring multiple variables, the Neon NxT 8-Channel Pipette provides the versatility needed to accelerate your research and achieve more reliable results.

product photo

Flexibility to maximize optimization

With the Neon NxT 8-Channel Pipette where each channel can be a different condition, you can efficiently test a wide range of parameters in a single experiment, greatly enhancing your research throughput and precision. This streamlined approach allows for rapid and accurate optimization, ensuring high efficiency and viability in your experiments (Figure 4).

graph of GFP cells vs total number of viable transfected cells

Figure 4. Neon NxT transfection program optimization study. We evaluated the optimal electroporation program and resuspension buffer for 11.5 kb GFP plasmid transfection in Jurkat cells. Each data point, representing a specific electroporation program, shows the percentage of GFP-positive cells 24 hours post-electroporation against the total number of viable transfected cells (TVTC). The red circles highlight the best-performing conditions, achieving up to 85% transfection efficiency with T buffer.



Minimize sample transfer loss and contamination risk of valuable cells with unique and compact electroporation instrument design

Minimal sample transfer loss

Your samples are precious, but some volume is inevitably lost during transfer to and from a conventional electroporation cuvette. Electroporation on the Neon NxT Electroporation System occurs within the pipette tip, helping to minimize sample loss and the amount of shear force experienced by cells.


Minimal sample contamination

The compact footprint of the Neon NxT system, including the pulse generator and pipette station, makes it small enough to fit in most biosafety cabinets (BSCs). Your samples always stay within the aseptic area while using the instrument, helping reduce the risk of contaminating precious cells. The Neon NxT system also has a new cable management feature that makes using it in the BSC hassle-free.



Specification comparison between the Neon NxT Electroporation System and the Neon Transfection System

SpecificationPhotograph showing the Neon NxT Electroporation System
Neon NxT Electroporation System
Photograph showing the Neon Transfection System
Neon Transfection System
Pipette type 1-channel and 8-channel options1-channel only
Electroporation Volume10 µL or 100 µL10 µL or 100 µL
Electroporation buffer volume*2 mL3 mL
Tip attachmentClipTip technologyFriction
Electroporation pulses1–101–10
Pulse duration1–100 ms1–100 ms
Pulse voltage500–2,500 V500–2,500 V
Arc detectionYesNo
Cloud connectivityYesNo
Pulse generator dimensions**9.5 x 7.6 x 9.9 in. (W x H x D)
11. 9 lb (5.4 kg)
9.5 x 8.9 x 13.6 in. (W x H x D)
13. 8 lb (6.25 kg)
Cable management feature***YesNo
Touch display8-inch capacitive touchscreen7-inch touchscreen
Electrical rating100–240 VAC, 270 W100–240 C, 150 W

*The buffer tube of the Neon NxT Electroporation System has a 2 mL level indicator.
**The Neon NxT pulse generator can be moved into or out of a typical BSC without removing the sash.
***Excess cable length can be secured behind the Neon NxT system with the attachable cable organizer.

The same technology and performance as the Neon Transfection System

Multiple bar graphs comparing transfection efficiency and cell viability between the Neon NxT Electroporation System and the Neon Transfection System. Panels A and B show similar transfection efficiencies and cell viabilities between the two electroporation instruments for Jurkat, BC-1, HeLA, HEK293, and Primary activated T cells transfected with GFP DNA. Panels C and D show similar transfection efficiencies and cell viabilities between the two instruments for Naïve T, Jurkat, and HEK393T cells transfected with GFP mRNA.

Figure 6. Performance of the Neon NxT Electroporation System and the Neon Transfection System. Performance was evaluated by transfecting different mammalian cell lines with GFP plasmid DNA or GFP mRNA. (A) GFP plasmid DNA transfection efficiency reported as the percentage of GFP-positive cells. (B) Viability of cells after transfection with GFP plasmid DNA. (C) GFP mRNA transfection efficiency reported as the percentage of GFP-positive cells. (D) Viability of cells after transfection with GFP mRNA.


Gibco CTS Xenon Electroporation System for Cell Therapy Manufacturing

A closed, scalable electroporation system for GMP-compliant cell therapy manufacturing. Process- and protocol-compatible with the Neon NxT Transfection System, the Xenon system helps enable smooth scale-up from research to process development to commercial manufacturing of up to 25 mL at a time.

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Transfection FAQs

Search our extensive collection of Transfection FAQs to find the answers related to Neon Electroporation Transfection.

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