Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis by disrupting translocation and promoting mistranslation at the 80S ribosome. Because it uses a different mode of action than Gibco Geneticin, Blasticidin S, or Gibco Zeocin, it’s perfect for dual-selection experiments when used in conjunction with another selection agent.
Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). The concentration for selection ranges from 100-1000 µg/ml (typically 200 µg/ml) and should be optimized for each cell line.
FW: 527.5 g/mole
Table 1 - Selection agents and resistant vectors
|Cell types||Conc. (μg/ml)*||Invitrogen vectors carrying resistance marker|
|HeLa||~550||pcDNA5 vectors (Constitutive mammalian expression)|
|CHO||~250||pIND/Hygro vector (Ecdysone-Inducible mammalian expression)|
|Jurkat T-Cell||~1,000||pSecTag2/Hygro vector (Secreted mammalian expression)|
|S2 Drosophila||200-300||pREP vectors (Episomal mammalian expression)|
|pCoHygro (Selection vector for DES)|
* The optimal concentration for selection of your cell line should be determined using kill curves.