Lipofectamine LTX Reagent is a plasmid transfection reagent that offers a balance of potency and gentleness for your cells, resulting in high transfection efficiencies and viabilities. This reagent provides an advanced solution for gene expression in common cell lines, particularly Chinese Hamster Ovary (CHO) cells, and challenging cell types such as primary neural progenitor cells, primary fibroblasts, and primary epithelial cells.
In addition to being excellent for transfecting primary and difficult-to-transfect cells, Lipofectamine LTX reagent provides a versatile approach to transfecting established cell lines, including those that are sensitive to other transfection reagents. Data for Lipofectamine vs FuGENE® has shown that the Lipofectamine LTX reagent leads to exceptional transfection efficiency with lower toxicity (Figure 1).
Figure 1. Lipofectamine LTX Reagent combines high transfection efficiency with low cytotoxicity superior to FuGENE 6 and FuGENE HD transfection reagents. Activity/toxicity analysis measures the ratio of peak gene expression to cytotoxicity, with higher ratios indicating optimization of transfection efficiency with low cytotoxicity levels. Ten cell lines were transfected with luciferase-expressing plasmids following recommended protocols for the respective reagents. Cells were analyzed for toxicity using the Invitrogen CyQUANT Cytotoxicity Assay Kit (Cat. No. V23111) and assayed for luciferase expression. The data were normalized to the activity/toxicity ratios for the FuGENE HD reagent.
Primary cells have traditionally been difficult to transfect. Lipofectamine LTX reagent, when combined with PLUS Reagent, solves the problem of transfecting primary cells, including primary neural progenitor cells (Figure 2).
Figure 2. Lipofectamine LTX reagent with PLUS Reagent efficiently transfects primary neural progenitor cells. Lipofectamine LTX reagent (1.5 μL) was used to transfect murine embryonic primary neural progenitor cells with the indicated quantities of a GFP-expressing plasmid in the presence of PLUS Reagent (2.5 μL). GFP expression was analyzed 24 hours post-transfection. Data courtesy of the Beverly Davidson laboratory, University of Iowa.
Lipofectamine LTX reagent has a protocol that is easy to use with improved performance. When using this protocol, there is no need to remove transfection complexes or change medium following transfection. You can spend less hands-on time, and get better results, faster.
Figure 3. Comparison of performance between the current protocol with an old protocol for use of Lipofectamine LTX PLUS Reagent.
How to perform plasmid DNA transfection with Lipofectamine LTX with PLUS Reagent
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