A DNA library is a collection of DNA fragments that have been cloned into vectors so that researchers can identify and isolate the DNA fragments that interest them for further study. There are basically two kinds of libraries: genomic DNA and cDNA libraries. Genomic DNA libraries contain large fragments of DNA in either bacteriophages or bacterial or P1-derived artificial chromosomes (BACs and. PACs). cDNA libraries are made with cloned, reverse-transcribed mRNA, and therefore lack DNA sequences corresponding to genomic regions that are not expressed, such as introns and 5′ and 3′ noncoding regions. cDNA libraries generally contain much smaller fragments than genomic DNA libraries, and are usually cloned into plasmid vectors.
We offer genomic DNA libraries as well as innovative Invitrogen products for the construction of full-length cDNA libraries from your source material, PCR-ready first-strand cDNA from various tissue sources, and RACE kits.
Construct high-quality full-length cDNA libraries using SuperScript reverse transcriptase and Gateway recombination cloning (no restriction enzyme digestion needed), or use standard restriction enzyme–based cloning.
Gene Pool cDNA is PCR-ready, first-strand cDNA for specific or degenerate primers, analysis of tissue- or tumor-specific expression, or characterization of spliced mRNA.