What are isoschizomers?

Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. The prototype enzyme is generally the first to be discovered and characterized. Subsequent enzyme(s) discovered from different hosts that recognize and cut at the same target sequence are termed “isoschizomers” of that prototype. Because enzyme naming conventions reference the genus, species, and strain from which they were isolated, isoschizomers could have very different names, despite the sequence recognition similarities between them. For example, Anza 3 BcuI is an isoschizomer of SpeI. Review the table below outlining common Anza isoschizomers offered in our Anza Restriction Enzyme Cloning System.

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PrototypeAnza isoschizomerRecognition and cutting site
SpeIAnza 3 BcuIA^CTAG T
T GATC^A
AgeIAnza 7 BshTIA^CCGG T
T GGCC^A
NdeIAnza 9 NdeICA^TA TG
GT AT^AC
AvrIIAnza 15 XmaJIC^CTAG G
G GATC^C
AscIAnza 21 SgsIGG^CGCG CC
CC GCGC^GG
PmeIAnza 24 MssI
GTTT^AAAC
CAAA^TTG
SphI

Anza 25 PaeI

G CATG^C
C^GTAC G
EcoRVAnza 26 Eco32IGAT^ATC
CAN^TAG
ClaIAnza 30 Bsu15IAT^CG AT
TA GC^TA
MfeIAnza 31 MunIC^AATT G
G TTAA^C
SapIAnza 33 LguIGCTCTTCN1^
CGAGAAGN4^
DdeIAnza 41 HpyF3IC^TNA G
G ANT^C
SnaBIAnza 43 Eco105ITAC^GTA
ATG^CAT
SwaIAnza 49 SmiIATTT^AAAT
TAAA^TTTA
HpaIAnza 50 KspAIGTT^AAC
CAA^TTG
PvuIAnza 52 PvuIICG AT^CG
GC^AT GC