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A complete collection of single-use and high-throughput electrocompetent and chemically competent E. coli

Choosing the ideal competent cells for your cloning applications and workflows is a critical component of success. Aspects to consider when selecting your competent E. coli strain include:

  • Transfection method – chemical versus electroporation
  • Transformation efficiency – bacterial strain and genotype
  • Downstream application – protein expression, library production, general cloning

Find the products you need to get started

Quickly find the right competent cell strain that fits your application and your budget. Find the right strain for your research. Each E. coli strain is specially engineered to keep you moving ahead.

ApplicationE. coli strainAvailable as high efficiency  ( >10 9 cfu/μg)
Routine cloning / subcloningDH5α Family*> 1 x 109
DH10B Family*> 1 x 109
TOP10 Family*> 1 x 109
OmniMax 2 T1R*> 5 x 109
Mach1 T1R*> 1 x 109
Protein expressionBL21 Family*N/A
Cloning unstable DNAStbl2, Stbl3*, Stbl4 

Stbl2: > 1 x 109

Stbl4: > 5 x 109

Library productionDH10B Family > 1 x 1010
Stbl4 > 5 x 109
OmniMax 2 T1> 5 x 109
ssDNA propagationDH12S > 1 x 1010
Bacmid creationDH10BacN/A
Cre-lox recombination PIR1 / PIR2PIR1: > 1 x 1010
Single Strand DNA Isolation  TOP10 F' / TOP10 F'

TOP10 F': > 1 x 109

TOP10 F' (LB): > 5 x 109

INVαF'N/A
Highest efficiencyMegaX DH10B T1R > 3 x 1010
Plasmid prep  of SupF-containing vectors  TOP10/P3> 1 x 109
MC1061/P3N/A
Plasmid purification of dam or dcm methylation sensitive restriction enzymesINV110N/A
Use with Gateway vectors and plasmids with ccdB geneccd B Survival T1R> 1 x 109
*Certain E. coli strains within the family are available in a high-throughput configuration.
⚡ = Electrocompetent cells.

Available competent cell formats

MultiShot product line overview

Resources

Cloning Support Center
Find tips, troubleshooting help, and resources for your
cloning applications

Competent cells resources