Gateway Cloning

The easy-to-use choice for everyday cloning

Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system in just a few simple steps. From protein expression to functional analysis, Gateway® cloning technology is applicable for a variety of research areas, for truly multidisciplinary scientific studies.

Single-step BP/LR Clonase® reaction protocol now available!

  • Fast—1-hour room temperature cloning reactions
  • Accurate—cloning efficiency >99% delivers the clone you need
  • Easy—no need for restriction enzymes or ligation to maintain orientations and reading frames for expression-ready clones
  • Convenient—no resequencing required; use the same clone from target identification to validation for consistency
  • Flexible—shuttle DNA inserts from one expression vector to the next
  • Versatile—select from E. coli, yeast, insect, or mammalian cell Destination vectors (see the basics of Gateway® cloning reactions)

Learn more

Single-step BP/LR Clonase® reaction protocol

DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

  • Reduce the overall time needed to obtain an Expression clone from 3 days to 1
  • Reduce the cost by using less BP Clonase® enzyme

This protocol has been published in BioTechniques.
Download the PDF.
Download the Supplementary Material and Protocol.

Meet the Inventor video series

Widely adopted in the research community with more than 1,500 references since its launch, Gateway® cloning technology makes collaboration across research disciplines easy and convenient.

Dr. Jon Chesnut
Gateway® Cloning Technology

NEW Cloning Support Center
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