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Truncation Without Frameshifts

In a truncation library, the wild type sequence can be shortened from both ends in single amino acid/single codon steps.

GeneArt™ Truncation Libraries consist of a pool of DNA fragments of different length that all contain a certain core region of the gene of interest. Unlike other methods, our process preserves the open reading frame, avoiding out-of- frame mutations.

Truncation libraries can for example be used to:

  • Optimize solubility
  • Evaluate minimal functional-protein size
  • Identify functional domains
  • Screen for inhibitors
  • Map epitopes

Get your project started

Please download the questionnaire to submit project information. For secure data transfer please register at our
customer portal.

For further information regarding this service or the order process, please contact geneartsupport@thermofisher.com.

GeneArt™ Truncation Libraries are cloned into the vector of your choice, then up to 96 clones are peer group–sequenced to help ensure that your library meets your specifications.

  • 30 µg of plasmid DNA cloned into the vector of your choice
  • Peer group sequencing of up to 96 clones
  • Glycerol stock of transformed cells
  • More functional variants
    • No system-based out-of-frame mutations
    • Low ancillary mutation rates—>90% sequence integrity (depending on length of the gene; unintended mutants will have amino acid substitutions in most cases)
  • High success rates—receive all possible truncation variants; library diversity of up to ~40,000

For Research Use Only. Not for use in diagnostic procedures.