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HTP Strings DNA Fragments |
Normalized DNA fragments for high-throughput antibody construct generation
Invitrogen GeneArt HTP Strings DNA Fragments are designed to easily integrate into your high throughput cloning workflow. These DNA fragments are normalized to enhance cloning efficiency and streamline the generation of expression constructs for antibody screening.
For orders of more than 500 DNA fragments, we recommend downloading the order form and sending it to the GeneArt support team for the most efficient handling.
High quality DNA for efficient cloning
To decrease screening effort, it is critical to use DNA fragments with low error rates in your high throughput cloning pipeline. GeneArt HTP Strings Fragments offer some of the lowest error rates on the market, helping improve your chances of picking the right clone the first time.
To demonstrate this advantage, a test set of 55 HTP Strings DNA fragments containing 49 heavy or light chain variable regions and 6 non-antibodies was assessed by next-generation sequencing and achieved an average of 95.1% correctness. To confirm the NGS results and demonstrate suitability of the product for the desired cloning application, 80 HTP Strings DNA Fragments (coding heavy and light chain variable regions) were cloned in appropriate vectors. For two clones of each sample, correctness was determined by PCR and sequencing. In total, 156/160 clones (97.5%) were correct. Moreover, at least one correct clone could be identified for all tested samples (100%).
Distribution of perfect molecules
Figure 1. A test set of 55 HTP Strings DNA fragments was sequenced by NGS to assess the proportion of fully correct sequences for each. LSL (lower specification limit) is the proportion of correct molecules needed to identify ≥1 correct clone when screening two clones.
Cloning performance
Figure 2. A test set of 80 HTP DNA fragments (48 LC variable regions, 32 HC variable regions) was cloned into pcDNA3.4 containing antibody constant regions using Golden Gate cloning. Sequence verification by colony-PCRs of two clones each.
Sequence optimization to enhance downstream expression
For greater efficiency throughout the research and development workflow, strategies to maximize protein expression should be considered at the design stage. Species-matched codon usage and other sequence factors can greatly impact final protein yield. The Invitrogen GeneArt GeneOptimizer algorithm enables sequence optimization to help improve your downstream results and can be applied when designing and ordering HTP Strings DNA Fragments online.
Flexible delivery format for HTP DNA Fragments
Help reduce liquid handling steps by choosing the ready-to-use delivery format that meets your needs. Select from several standard 96-well storage plates or 2D-barcoded tube options, including Thermo Scientific Matrix tubes in 96 SBS format.
- Define custom double-stranded DNA fragments for your specific cloning strategy
- Customize plate layout
- Fragments provided in water or TE buffer
Additional delivery formats available upon request.
| Length range | 200–450 bp |
| Yield | 400 ng |
| Normalization | Concentration: 10 ng/µL |
| Buffer options |
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| Delivery format | Frozen |
| Sequence verification | Next-generation sequencing |
| Supported cloning methods | Golden Gate (scarless), restriction enzymes |
| Adapters | Fragments contain 38 bp universal linkers at each end, eliminating the need to add stuffer sequences outside terminal cloning sites |
| Minimum order size | 192 sequences |
| Production time | 5–6 business days |
Additional services for antibody engineering
For Research Use Only. Not for use in diagnostic procedures.
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