Protein Production Services
Fast, reliable protein production from mammalian cells
The GeneArt™ Genes-to-Proteins service is an extremely fast way to obtain correctly folded, native protein from transiently transfected mammalian cells. Starting with only the nucleotide sequence, we can provide purified protein typically within 30 business days. We clone your expression optimized gene into one of the advanced Life Technologies expression vectors, produce transfection grade plasmid DNA and then use the described advanced expression systems and conditions to obtain high expression yields. Secreted or intracellular protein is then purified using affinity chromatography (e.g. Fc tag, His tag, etc.). Further protein polishing steps are available if highly purified protein is needed. A detailed documentation including coomassie stained PAGE gel and western blot is provided with every purified protein. Deliverables of such a project are the protein of interest and the expression vector used for transfection.
Table 1: Protein production services; optimally combined with gene synthesis and subcloning into desired Life Technologies expression vector to achieve highest expression yields.
|Genes-to-Proteins Pilot||Feasibility study for determination of production yield of protein from transiently transfected HEK293 or CHO cells||
|Genes-to-Proteins Purification||Protein expression and purification from customer specified culture volume of transiently transfected 293 or CHO cells||
Protein expression and purification of customer specified protein amount using transiently transfected 293 or CHO cells
Pilot expression service mandatory
The combination of GeneArt™ expression optimization and Life Technologies advanced expression systems (e.g. Expi™293) usually lead to higher overall project reliability and expression yields than obtained with non-optimized genes and other expression systems (see section Expi 293 expression system).
Figure 1: Advantages of the GeneArt™ Genes-to-Proteins service.
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For Research Use Only. Not for use in diagnostic procedures.