Making a gene or genetic elements can be done through cloning with TOPO technology, constructing the gene with the GeneArt Seamless Cloning and Assembly Kit or purchasing an optimized GeneArt gene. TOPO cloning is the ideal solution for the researcher who needs to clone genes with a high cloning efficiency. The GeneArt seamless cloning allows a researcher to clone up to 4 fragments directly into any vector of their choice with high efficiency in a 30 minute bench-top cloning reaction. GeneArt Optimized Genes are the most complete solution for customers, providing what they need with a minimum of hand-on time.

GeneArt Seamless Cloning and Assembly Kits work great together with GeneArt Strings DNA Fragments. This new service offers custom-made DNA fragments up to 1,000 bp ready for cloning and assembly and delivered in only 5-7 business days.

Making a Gene or Genetic Element

 Quick & simple cloning when constructing a geneThe highest efficiency, direct cloning technology available todayCustom optimized gene, 100% accurate, no limitation in sequence design
 TOPO Cloning
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GeneArt Seamless Cloning and Assembly Kit & GeneArt Strings DNA Fragments
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GeneArt Optimized Genes
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Key FeaturesFastest cloning method with high efficiency into a cloning vectorCan clone up to 4 fragments into any vector without additional sequenceFull service that delivers validated Genes with improved expression yields, no limitation in sequence design
Time spent cloning5 minutes30 minutesNo hands-on time required at all
Maximum Number of Fragments14Service can deliver length of 20kb and more
Cloning Efficiency95%90% (single fragment);
75% (4 fragments)
Service can deliver length of >20kb
Time to Clone Multiple FragmentsN/A1–3 days6–15 days to receive clones ready for use
Improvement of expression yieldsN/AN/AUp to 100%
Webtool for oligo and construct designN/AFree!Free!
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For Research Use Only. Not for use in diagnostic procedures.