strand issue 1

In This Issue

gene synthesis  

 Featured Video

Synthetic Biology in a Nutshell
Enjoy this TEDx presentation with Dr. Ralf Wagner of Life Technologies....read more>>

     

 

New Tools: GeneArt® Precision TALs: Precise Genome Engineering

GeneArt® Precision TALs provide custom DNA-binding proteins for accurate DNA targeting ...read more>>
 
     
NewCloning Tools  

Tips & Tricks

Top 5 Gateway® Cloning Tips & Tricks ..read more>>

 

bioinformatics  

The Future of Bioinformatics

Test drive the future of bioinformatics
Vector NTI® Express software supports the future of bioinformatics and synthetic biology with a plug-in architecture to consistently deliver new tools...read more>>

     
resources  

Synthetic Biology Resources

Keep up to date with the latest synthetic biology products and applications...read more>>

     

 

Did you know?

Life Technologies sequencer identifies European E. coli


What can SynBio do for you? In 2011, the Life Technologies ION PGM Sequencer was used to resolve a major E. coli health crisis. read more>>


Upcoming Events

Come see us at the upcoming Trade shows & Conferences...see where>>


Q&A

Bioinformatics
Learn the latest in our bioinformatics portfolio with Raymond Tecotzky. read more>>


Hot from Social

Recombination-Based DNA Assembly and Mutagenesis Methods.....read more>>

Featured Video

Synthetic Biology in a Nutshell

Enjoy this TEDx presentation with Dr. Ralf Wagner of Life Technologies.

Dr. Wagner was one of the first to realize the enormous scientific and commercial potential of synthetic biology. He successfully converted that scientific and technical vision into a sound business model supplying gene optimization, DNA synthesis and subcloning to the 20 largest pharmaceutical companies in the world. In addition to his work at GeneArt®, Ralf also manages the Molecular Microbiology and Gene Therapy unit at the University of Regensburg. Some people decide to build a better mouse trap. Others, a better mouse.

 

GeneArt® Precision TALs: Precise Genome Engineering

 

GeneArt® Precision TALs provide custom DNA-binding proteins for accurate DNA targeting and precise genome editing. Other technologies limit the choice of targets, but with GeneArt® Precision TALs, you can target any locus in the genome, and this increase in engineering accuracy allows you to develop better solutions for healthcare, agriculture, energy, and bioproduction research.

Start editing

Tips & Tricks

Top 5 Gateway® Cloning Tips & Tricks

gateway cloning 
  1. Recovering an entry clone from an expression construct. The efficiency of the BP reaction can be improved by linearizing the expression construct. The BP Clonase® works more efficiently when, at least, one of the substrates is a linear fragment.
  2. Improve the chances to obtain PCR amplified fragments for BP reactions. Relatively short template specific sequence (12-14 mer) improves the chances of amplification success without compromising specificity.
  3. Use destination vectors and entry clones with the same selection marker. It can be performed by linearizing the entry clone with one or more restriction enzymes that do not cut within the insert sequence.
  4. Give a second chance to failed LR or BP reactions. Do not stop the reaction with proteinase K. Instead, after transformation, keep the tube at room temperature and re-transform the following day if necessary.
  5. Speed up the process. Use One Shot® Mach1™-T1R Chemically Competent E. coli cells. Colonies are clearly visible after eight hours of plating. Transformants to be selected on ampicillin plates do not need to be recovered before plating.
Have tips and tricks you use in your lab?  Send us your tips

Test drive the future of bioinformatics

Vector NTI® Express software supports the future of bioinformatics and synthetic biology with a plug-in architecture to consistently deliver new tools and functionality. Life Technologies can provide a customized Vector NTI® Express software quote to meet your needs.

Receive a custom quote

   

Learn the latest in our bioinformatics portfolio with Raymond Tecotzky, product manager of Vector NTI® software.


Question: What’s the difference between the Vector NTI Advance® and Vector NTI® Express software suites?

Answer: Both the Vector NTI Advance® and Vector NTI® Express software suites allow you to manage, view, analyze, transform, share, and publicize diverse types of molecular biology. The Vector NTI Advance® sequence analysis suite has been around for more than 20 years, while the Vector NTI® Express software is a newer, even faster, simpler, and easier-to-use version of Vector NTI® software. Vector NTI® Express software is also designed to run on both PC and Mac® platforms. You can find a full list of Vector NTI® software features here.

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