Strand Newsletter

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Research of Note

Genetic Assembly Tools for Synthetic Biology

Billyana Tsvetanova et al present a set of protocols for “seamless, simultaneous, flexible, and highly efficient assembly....read more>>

 

new tools

 

 

New Tools

Life Technologies launches new DNA molecule design tool

The free GeneArt® Primer and Construct Design Tool facilitates the in silico design, assembly,
...read more>>

   
bioinformatics 

 Meet R&D: GeneArt® Precision TALs

....watch video>>
   
 Technology Applied 

Technology Applied

Learn about GeneArt® Directed Evolution Services from product manager Christian Kranz.....read more>>

Did you know?

The FDA approved the first biosynthetic insulin for sale on October 28, 1982.

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Upcoming events

Come see us at these upcoming Trade shows & Conferences

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Synthetic Biology Resources

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Research of Note

Genetic Assembly Tools for Synthetic Biology        

Genetic Assembly Tools for Synthetic Biology

Billyana Tsvetanova et al present a set of protocols for “seamless, simultaneous, flexible, and highly efficient assembly of genetic material, designed for a wide size dynamic range.”1 The authors explore the potential for applying the method in vitro or within living cells.
1Billyana Tsvetanova et al., “Genetic Assembly Tools for Synthetic Biology,” Methods in Enzymology 498 (2011), p. 328.

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Send us your story on how Life Technologies is enabling you to make breakthroughs

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   The free GeneArt® Primer and Construct Design Tool facilitates the in silico design, assembly, or mutagenesis of a DNA molecule using GeneArt® technology. Use the GeneArt® design tool in conjunction with GeneArt® Seamless Cloning and Assembly Kits and GeneArt® Mutagenesis Kits—the tool helps you with elements of the workflow protocol including fragment import, order, and orientation, as well as primer creation for precloning, fragment assembly, or mutagenesis.

Get the design tool now


The GeneArt® GeneOptimizer® process significantly increases protein expression.

The GeneOptimizer® process has been shown to help deliver up to 100-fold increases in protein yield due to a combination of parameters, including codon optimization, that stabilize mRNA and maximize translational efficacy (Figure 1)

JNK3

Figure1. Relative JNK3 protein expression levels of wild type protein, and JNK3 with gene sequence optimized by the GeneArt® GeneOptimizer® process (1) (1) Fath S, Bauer AP, Liss M et al. (2011), PLoS One 6(3):e17596.


GeneArt Gene Synthesis

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Meet R&D: GeneArt® Precision TALs

Named the scientific method of the year in 2011 by Nature Methods, gene-editing nucleases have created new possibilities for the study of genes and custom genomes. Meet one of our research fellows and hear him explain the use and benefits of TAL effector proteins, a method of gene editing.

Trade shows & Conferences

Come see us at these upcoming Trade shows & Conferences

Event     Date
13th International Conference on Systems Biology (ICSB) Aug. 2012
Algae Biomass Organization (ABO) Summit Sept. 2012
FASEB Genome Engineering—Science Research Conference Sept. 2012

Technology Applied Q&A

 

Learn about GeneArt® Directed Evolution Services from product manager Christian Kranz.

Q: How can I benefit from GeneArt® Directed Evolution Services?

CK: Directed evolution helps you to modify selected protein properties, like antibody affinity or thermostability of industrial enzymes. GeneArt® Directed Evolution Services provide a toolbox with various mutagenesis tools. Mutagenesis can be fairly simple, such as adding an affinity tag to a certain construct or introducing a deletion or substitution at a specified site. On the other hand, mutagenesis can also be complex. We have technologies like the TRIM technology that enable us to provide you with billions of mutants (mutant libraries), that can be used to screen for the specimen with improved properties.

To get more information on directed evolution, visit the website.

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