TOPO PCR Cloning
Twenty years ago, the TOPO name became synonymous with superior quality and game-changing innovation in PCR cloning. Today, Invitrogen TOPO cloning kits are still the fastest, simplest, and most efficient cloning solutions available. There is no substitute.
- Fast—5-minute, room temperature reaction
- Simple—add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps
- Efficient—up to 95% of clones contain desired insert
- Flexible—available in a variety of formats and sizes to suit your choice in polymerase
- Proven—20,000 citations
No matter what your downstream application is, TOPO PCR cloning solutions help you get the right clone sooner, freeing up your time to answer more important questions.
TOPO Tip of the Month
Eliminate blue/white screening with TOPO Zero Blunt vectors, which contain a lethal ccdB gene. When disrupted by the insertion of a PCR product, only positive recombinants are permitted to grow upon transformation.
See how TOPO PCR Cloning technology works
Learn how simple it is to get 95% recovery of your clone in just 5 minutes.
Choose the right TOPO cloning solution for you
For easy, highly efficient cloning of extra-long PCR products (up to 13kb) using the new linearized, topoisomerase I-activated pCR™-XL-2-TOPO vector.
Meet the Inventor
Find out how the revolutionary TOPO cloning was developed with Dr. Jon Chestnut, research fellow in Synthetic Biology R&D.
Dr. Jon Chesnut
TOPO Cloning Technology
For Research Use Only. Not for use in diagnostic procedures.