TOPO PCR Cloning

Twenty years ago, the TOPO name became synonymous with superior quality and game-changing innovation in PCR cloning. Today, Invitrogen TOPO cloning kits are still the fastest, simplest, and most efficient cloning solutions available. There is no substitute.

TOPO PCR Cloning Handbook

TOPO cloning technology highlights

  • Fast—5-minute, room temperature reaction
  • Simple—add restriction sites and/or universal primer sites to either end of your PCR product in just 3 easy steps
  • Efficient—up to 95% of clones contain desired insert
  • Flexible—available in a variety of formats and sizes to suit your choice in polymerase
  • Proven—20,000 citations

No matter what your downstream application is, TOPO PCR cloning solutions help you get the right clone sooner, freeing up your time to answer more important questions.

TOPO Tip of the Month

Eliminate blue/white screening with TOPO Zero Blunt vectors, which contain a lethal ccdB gene. When disrupted by the insertion of a PCR product, only positive recombinants are permitted to grow upon transformation.

Choose the right TOPO cloning solution for you

Invitrogen TOPO PCR cloning technology was developed to help improve cloning efficiency, simplify protocol setup, and accommodate a wide range of PCR insert sizes. TOPO technology enables inserts with compatible ends to be readily joined to the vector in 5 minutes, without the need for additional ligation steps. Learn more about our TOPO cloning solutions below.

For the direct insertion of blunt-end PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Now with 25% extra reactions.

For the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning. Now with 25% extra reactions.

For easy, highly efficient cloning of extra-long PCR products (up to 13kb) using the new linearized, topoisomerase I-activated pCR™-XL-2-TOPO vector.

TOPO Kits without competent cells

Now with 25% extra reactions.

Gateway recombinant cloning with TOPO Technology

Expression cloning with TOPO Technology


Featured videos

See how TOPO PCR Cloning technology works

Learn how simple it is to get 95% recovery of your clone in just 5 minutes.

Meet the Inventor

Find out how the revolutionary TOPO cloning was developed with Dr. Jon Chestnut, research fellow in Synthetic Biology R&D.

Dr. Jon Chesnut
TOPO Cloning Technology


Cloning Support Center
Find tips, troubleshooting help, and resources for your cloning applications

Cloning Education
Learn about PCR cloning strategies and subcloning approaches

PCR Education
Review the principles of PCR, components, cycling parameters, methods, and applications