All Thermo Scientific™ KingFisher™ instruments come with dedicated Thermo Scientific™ BindIt™ Software (PC), which includes optimized protocols for a variety of Invitrogen™ and Thermo Scientific™ purification kits and reagents. Here, we provide users with access to download new protocols for free as they become available. Choose protocols designed for different protein purification procedures and reagent kits from various manufacturers.

First read the instructions for loading BindIt Software protocols (PDF, 20 KB)

Select and import protocols to your computer:

  1. Locate the desired Software Protocol in the table below.
  2. Save the “.msz” or “.bdz” file to your computer using mouse right click. Note: Protocols that were made with KingFisher Software cannot be imported to a PC with the BindIt Software.
  3. In the Home menu of the BindIt Software, click Import on the action panel. The Import Data dialog opens.
  4. Click Browse to select the file (.msz) that you want to import from a directory.
  5. Select the desired file, and click Open. Then click Next.
  6. Select a location to import the protocol. You can also create a new folder and rename it.
  7. Click Finish. A message appears on the toolbar informing if the import was successful.

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† Strategy (Indirect or Direct). This refers to the order of binding steps, and it primarily pertains to immunoprecipitation protocols. Indirect protocols involve incubating the target-containing sample and antibody first, and then incubating with the beads second. Direct protocols involve incubating beads and antibody first (either to bind or covalently couple the IP antibody), and then incubating the bead-antibody complex with the sample.

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Ligand Product Protocol name: description Strategy† Download file
Protein A Dynabeads Protein A IP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Protein A Dynabeads Protein A IP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex | Duo Prime
Protein A Dynabeads Protein A Immunoprecipitation Kit IP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Protein A Dynabeads Protein A Immunoprecipitation Kit IP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex | Duo Prime
Protein G Dynabeads Protein G IP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Protein G Dynabeads Protein G IP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex | Duo Prime
Protein G Dynabeads Protein G Immunoprecipitation Kit IP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Protein G Dynabeads Protein G Immunoprecipitation Kit IP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex | Duo Prime
Sheep anti-Mouse IgG Dynabeads Sheep anti-Mouse IgG IP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Sheep anti-Rabbit IgG Dynabeads Sheep anti-Rabbit IgG IP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Streptavidin Dynabeads M-280 Streptavidin IP Dynabeads M-280 SA Direct:
Immunoprecipitation (IP or Co-IP)
Direct Flex | Duo Prime
Glutathione Pierce Glutathione Magnetic Agarose Beads New Purify GST tag Magnetic Agarose GSH Direct:
GST fusion protein purification
Direct Flex | Duo Prime
Ni-NTA Pierce Ni-NTA Magnetic Agarose Beads New Purify His tag Magnetic Agarose Ni-NTA Direct:
His-tagged fusion protein purification
Direct Flex | Duo Prime
Protein A/G Pierce Protein A/G Magnetic Agarose Beads New Purify Ab Magnetic Agarose Protein AG Direct:
Antibody purification of IgG containing Fc region
Direct Flex | Duo Prime
Anti-HA Antibody Pierce Anti-HA Magnetic Beads IP HA tag Pierce anti-HA Ab Direct:
HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
Direct Flex
Anti-c-Myc Antibody Pierce Anti-c-Myc Magnetic Beads IP Myc tag Pierce anti-Myc Ab Direct:
Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
Direct Flex
Glutathione Pierce Glutathione Magnetic Beads Purify GST tag Pierce GSH Direct:
GST fusion protein purification or pull-down
Direct Flex
Ni-NTA HisPur Ni-NTA Magnetic Beads Purify His tag Pierce Ni-NTA Direct:
His-tagged fusion protein purification or pull-down
Direct Flex
NHS Ester Pierce NHS-Activated Magnetic Beads Couple Pierce NHS ester Direct:
Coupling (immobilization) of primary amine molecule (protein, peptide)
Direct Flex
NHS Ester Pierce NHS-Activated Magnetic Beads IP Pierce NHS ester Direct:
Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide)
Direct Flex
Protein A/G Pierce Protein A/G Magnetic Beads Purify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
Direct Flex
Protein A Pierce Protein A Magnetic Beads Purify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
Direct Flex
Protein G Pierce Protein G Magnetic Beads Purify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
Direct Flex
Protein L Pierce Protein L Magnetic Beads Purify Ab Pierce Protein AGL Direct:
Antibody purification of immunoglobulins containing kappa light chains
Direct Flex
Protein A/G Pierce Protein A/G Magnetic Beads IP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex
Protein A Pierce Protein A Magnetic Beads IP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex
Protein G Pierce Protein G Magnetic Beads IP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex
Protein A/G Pierce Classic Magnetic IP/Co-IP Kit IP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
Indirect Flex
NHS Ester Pierce Direct Magnetic IP/Co-IP Kit IP Pierce Direct IP Kit Direct:
Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads
Direct Flex
Protein A/G Pierce Crosslink Magnetic IP/Co-IP Kit IP Pierce Crosslink IP Kit Direct:
Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads
Direct Flex
Protein A/G Pierce Magnetic ChIP Kit ChIP Pierce ChIP Kit Indirect:
Chromatin immunoprecipition (ChIP) of protein-DNA complexes
Indirect Flex
Streptavidin Pierce Streptavidin Magnetic Beads IP Pierce SA heated elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution
Indirect Flex
Streptavidin Pierce Streptavidin Magnetic Beads IP Pierce SA low pH elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution
Indirect Flex
Titanium Dioxide (TiO2) Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit Enrich pPep Pierce pPep Kit Direct deepwell plate:
Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
Direct Flex
Titanium Dioxide (TiO2) Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit Enrich pPep Pierce pPep Kit Direct PCR plate:
Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
Direct Flex