Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots

Extract samples from dried blood spots for HIV-1 drug resistance detection

The Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots isolates and purifies nucleic acid from dried blood spots (DBS). Dried blood spot is an alternative specimen type that can be shipped at ambient temperature. This cost-effective solution is robust and easily transported. DBS extraction offers a simple and reliable solution for labs with limited resources. The Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is compatible with KingFisher automated purification.

The kit utilizes trusted MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing.

  • Cost-effective – boost testing volumes in remote regions
  • Flexible – can be used with DBS or plasma
  • Convenient – kit contains all reagents from extraction through isolation
  • High recovery and purity – due to improved sample mixing and washing with magnetic beads
  • Automated with the KingFisher purification system
     

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Product details for the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots

Reactions100 reactions
Test duration ~30 min DBS lysis + ~30 min viral RNA extraction
Hands-on time for 24 samples~30 min
Sample typesDried blood spots (DBS) and plasma
Viral concentration
  • HIV-1 positive DBS: >2,000 copies/mL
  • HIV-1 positive plasma: >1,000 copies/mL
Kit storage temperature15 °C to 25 °C
Required equipment
  • Magnetic stand (manual protocol)
  • KingFisher Sample Purification System (automated protocol)
Regulatory statusResearch Use Only (RUO)
Kit components
  • 60 mL Dried Blood Spot Lysis Solution
  • 55 mL Binding Solution
  • 100 mL Wash Solution
  • 10 mL Elution Solution
  • 1 mL Proteinase K
  • 2 mL DNA/RNA Binding Beads

Why use the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots?

  • Efficiency
    • Magnetic bead separation binds nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields.
    • Because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with protein-rich, large-volume samples such as whole blood or plasma that are commonly used for viral testing. 
  • Easy transport
    • DBS can be shipped at ambient temperatures. Reduce shipping costs and hassle by shipping samples through regular mail as nonhazardous materials.
    • Preserve sample integrity even in transport delays.
  • Robust
    • Collection can be performed in remote, resource-limited settings.
  • Small blood sample
    • DBS does not need special equipment for processing (whereas plasma requires centrifuge).
    • Requires less blood (50-100 µL per spot), which promotes patient comfort and makes samples easier to obtain.
  • HIV sample transportation
    • According to the World Health Organization (WHO), nearly one half of infants born to mothers infected with HIV have HIV drug resistance to one or more non-nucleoside reverse transcriptase inhibitors (NNRTIs). To test, samples are often collected far from the laboratory. Dried blood spot collection helps by being able to ship at ambient temperature.
       

Genotyping workflow used to detect HIV drug resistance

  1. Specimen Processing
  2. RNA Extraction
  3. RT-PCR
  1. Nested PCR
  2. Confirm & Purify PCR
  3. Cycle Sequencing
  1. Sequence Purification
  2. Sequence Detection
  3. Data Analysis