BindIt Software is free and provides automated protocols for KingFisher systems

Automated Protocols and Software

KingFisher automated purification systems are designed to adapt to your changing research needs. That’s why we include free software with all KingFisher instruments so that you can create, modify and store your protocols. You can also search and download automated protocols for our MagMAX nucleic acid purification kits, and Dynabeads and Pierce protein purification beads and kits.

KingFisher Flex and
Duo Prime Automated Protocol Software

KingFisher Flex and Duo Prime

Download BindIt Software

Features of Software

  • Compatible with KingFisher instruments
  • Conveniently create and modify protocols on your PC and transfer to them to your KingFisher instrument. The software allows you to define and edit steps and parameters for every part of your protocol—bind, wash, elute
  • Allows KingFisher plates and reagents to be defined in the plate layout
  • Generates status reports that include run log, plate layout, and step parameters

Instructions for downloading and installing protocols with BindIt software:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table (DNA/RNA protocols, Protein protocols). Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Upload and find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Already have a KingFisher Instrument and BindIt Software?

Download BindIt Software Upgrade

BindIx for
KingFisher Apex

KingFisher Apex

Features of Software

  • All of the functionality of the Apex instrument on your PC
  • Conveniently create and modify protocols
  • Easily transfer new or edited protocols to KingFisher Apex
  • Access protocol library
  • Available for PC laptops or desktop computers
  • Compatible with KingFisher Apex instruments only

How to install BindIx software*?

  1. Download BindIx here
  2. Download BindIx from Microsoft Store here
    BindIx from Microsoft Store can be installed by selecting “Install” button on MS Store.
  3. USB stick (delivered from manufacture)
    BindIx from USB stick can be installed by double-clicking .msix bundle file and after that selecting “Install” button.

Note: System requirements for BindIx software is Windows 10 version 1809 (17763.0) or higher.

DNA & RNA extraction protocols available to download by SKU

Nucleic acidSample typeKit nameSKU Format Software protocol
RNASalivaMagMAX Viral/Pathogen Kit (MVP I)A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
Flex
MagMAX Viral/Pathogen II Kit (MVPII)A48383up to 2,000 preps
DNABlood, buccal, swabsMagMAX DNA Multi-Sample UltraA25597
A25598
500 preps
2,500 preps
MagMAX DNA Blood Buccal
 Duo Prime Flex
DNABlood, buccal, swabsMagMAX DNA Multi-Sample Ultra w/ extra PK and beadsA25919
A25920
500 preps
2,500 preps
MagMAX DNA Blood Buccal
Duo PrimeFlex
DNABlood, buccal, swabsMagMAX DNA Multi-Sample441302050 prepsMagMAX DNA Blood Buccal
Duo PrimeFlex
DNABlood, buccal, swabsMagMAX-96 DNA Multi-Sample4413021
4413022
96 preps
5 x 96 preps
MagMAX DNA Blood Buccal
 Duo PrimeFlex
DNAPCR reactionChargeSwitch PCR Clean-Up KitCS12000
CS1200010
100 preps
960 preps
ChargeSwitch PCR reaction
 Flex
DNATissue, cellsMagMAX DNA Multi-Sample UltraA25597
A25598
500 preps
2,500 preps
MagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX DNA Multi-Sample Ultra w/ extra PK and beadsA25919
A25920
500 preps
2,500 preps
MagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX DNA Multi-Sample441302150 prepsMagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX-96 DNA Multi-Sample4413021
4413022
96 preps
5 x 96 preps
MagMAX DNA tissue cells
Duo PrimeFlex
RNA/DNAViralMagMAX Pathogen RNA/DNA4462359480 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Viral RNAAM183696 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Total Nucleic AcidAM1840100 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Pathogen RNA/DNA4462359480 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Viral RNAAM183696 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Total Nucleic AcidAM1840100 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNAViralMagMAX-96 AI/ND Viral RNA Isolation KitAM1835384 prepsMagMAX AI/ND Viral RNA
Duo PrimeFlex
RNA/
microRNA
Tissue, cells
blood, plasma, urine
MagMAX mirVana Total RNAA2782896 prepsMagMAX mirVana tissue cells
Duo PrimeFlex

MagMAX mirVana biofluids
Duo PrimeFlex
DNAPlasmaMagMAX Cell-Free DNAA2931996 prepsMagMAX Cell-Free DNA 2mL plasma
Duo PrimeFlex

MagMAX Cell-Free DNA 4mL plasma
Duo PrimeFlex
RNATissue, cells, plantsMagMAX-96 Total RNAAM183096 prepsMagMAX Total RNA tissue cell plant
Duo PrimeFlex
RNABloodMagMAX for Stabilized Blood (Paxgene tubes)445189496 prepsMagMAX for Paxgene tubes
Duo PrimeFlex
RNABloodMagMAX for Stabilized Blood (Tempus tubes)445189396 prepsMagMAX for Tempus tubes
Duo PrimeFlex
DNALeukocytesMagMAX DNA Multi-Sample UltraA25597500 prepsMagMAX DNA leukocytes
Duo PrimeFlex
mRNA Dynabeads mRNA Direct61011
61012
5 mL
10 mL
Dynabeads mRNA from crude samples
Duo PrimeFlex
RNA MagMAX for Microarrays Total RNA IsolationAM183996 prepsMagMAX for Microarrays with 100uL aqueous phase
Duo PrimeFlex
DNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra DNA only
Duo PrimeFlex
RNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra RNA only
Duo PrimeFlex
RNA/DNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra DNA and RNA
Duo PrimeFlex

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Uploadand find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Immunoprecipitation protocols for KingFisher Flex or DUO Prime automation

† Strategy (Indirect or Direct). This refers to the order of binding steps, and it primarily pertains to immunoprecipitation protocols. Indirect protocols involve incubating the target-containing sample and antibody first, and then incubating with the beads second. Direct protocols involve incubating beads and antibody first (either to bind or covalently couple the IP antibody), and then incubating the bead-antibody complex with the sample.

Ligand Product Protocol name: description Strategy† Download file
AntibodiesFabRICATOR MagIC immobilized on magnetic beadsHigh throughput parallel analysis of antibodiesIndirectFlex
Duo Prime
Protein ADynabeads Protein AIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein ADynabeads Protein AIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein ADynabeads Protein A Immunoprecipitation KitIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein ADynabeads Protein A Immunoprecipitation KitIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein GDynabeads Protein GIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein GDynabeads Protein GIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein GDynabeads Protein G Immunoprecipitation KitIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein GDynabeads Protein G Immunoprecipitation KitIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Sheep anti-Mouse IgGDynabeads Sheep anti-Mouse IgGIP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Sheep anti-Rabbit IgGDynabeads Sheep anti-Rabbit IgGIP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
StreptavidinDynabeads M-280 StreptavidinIP Dynabeads M-280 SA Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
GlutathionePierce Glutathione Magnetic Agarose BeadsPurify GST tag Magnetic Agarose GSH Direct:
GST fusion protein purification
DirectFlex
Duo Prime
Ni-IMACPierce High Capacity Ni-IMAC MagBeads, EDTA-compatiblePurify His tag Magnetic Agarose Ni-IMAC DirectDirectFlex
Duo Prime
Ni-NTAPierce Ni-NTA Magnetic Agarose BeadsPurify His tag Magnetic Agarose Ni-NTA Direct:
His-tagged fusion protein purification
DirectFlex
Duo Prime
Protein A/GPierce Protein A/G Magnetic Agarose BeadsPurify Ab Magnetic Agarose Protein AG Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Duo Prime
Anti-HA AntibodyPierce Anti-HA Magnetic BeadsIP HA tag Pierce anti-HA Ab Direct:
HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
DirectFlex
Anti-c-Myc AntibodyPierce Anti-c-Myc Magnetic BeadsIP Myc tag Pierce anti-Myc Ab Direct:
Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
DirectFlex
GlutathionePierce Glutathione Magnetic BeadsPurify GST tag Pierce GSH Direct:
GST fusion protein purification or pull-down
DirectFlex
Ni-NTAHisPur Ni-NTA Magnetic BeadsPurify His tag Pierce Ni-NTA Direct:
His-tagged fusion protein purification or pull-down
DirectFlex
NHS EsterPierce NHS-Activated Magnetic BeadsCouple Pierce NHS ester Direct:
Coupling (immobilization) of primary amine molecule (protein, peptide)
DirectFlex
NHS EsterPierce NHS-Activated Magnetic BeadsIP Pierce NHS ester Direct:
Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide)
DirectFlex
Protein A/GPierce Protein A/G Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein APierce Protein A Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein GPierce Protein G Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein LPierce Protein L Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of immunoglobulins containing kappa light chains
DirectFlex
Protein A/GPierce Protein A/G Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein APierce Protein A Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein GPierce Protein G Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein A/GPierce Classic Magnetic IP/Co-IP KitIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
NHS EsterPierce Direct Magnetic IP/Co-IP KitIP Pierce Direct IP Kit Direct:
Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads
DirectFlex
Protein A/GPierce Crosslink Magnetic IP/Co-IP KitIP Pierce Crosslink IP Kit Direct:
Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads
DirectFlex
Protein A/GPierce Magnetic ChIP KitChIP Pierce ChIP Kit Indirect:
Chromatin immunoprecipition (ChIP) of protein-DNA complexes
IndirectFlex
StreptavidinPierce Streptavidin Magnetic BeadsIP Pierce SA heated elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution
IndirectFlex
StreptavidinPierce Streptavidin Magnetic BeadsIP Pierce SA low pH elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution
IndirectFlex
Titanium Dioxide (TiO2)Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment KitEnrich pPep Pierce pPep Kit Direct deepwell plate:
Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
DirectFlex
Titanium Dioxide (TiO2)Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment KitEnrich pPep Pierce pPep Kit Direct PCR plate:
Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
DirectFlex

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Upload and find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Dynabeads virus enrichment and positive cell isolation protocols to download

LigandProductProtocol descriptionStrategyDownload file
VirusViruses (especially SARS-CoV-2), vesicles and proteins Dynabeads Intact Virus EnrichmentIndirectDuo Prime
VirusViruses (especially SARS-CoV-2), vesicles and proteinsDynabeads Intact Virus EnrichmentIndirectFlex
CellsCD3, CD4, CD8, CD14, CD19 Positive Cell Isolation for downstream nucleic acid,  protein analysis, or cell depletionIndirectDuo Prime
CellsCD3, CD4, CD8 Positive Cell Isolation for downstream cellular applications – such as T-cell ActivationIndirectDuo Prime

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Uploadand find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

KingFisher Flex and
Duo Prime Automated Protocol Software

KingFisher Flex and Duo Prime

Download BindIt Software

Features of Software

  • Compatible with KingFisher instruments
  • Conveniently create and modify protocols on your PC and transfer to them to your KingFisher instrument. The software allows you to define and edit steps and parameters for every part of your protocol—bind, wash, elute
  • Allows KingFisher plates and reagents to be defined in the plate layout
  • Generates status reports that include run log, plate layout, and step parameters

Instructions for downloading and installing protocols with BindIt software:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table (DNA/RNA protocols, Protein protocols). Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Upload and find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Already have a KingFisher Instrument and BindIt Software?

Download BindIt Software Upgrade

BindIx for
KingFisher Apex

KingFisher Apex

Features of Software

  • All of the functionality of the Apex instrument on your PC
  • Conveniently create and modify protocols
  • Easily transfer new or edited protocols to KingFisher Apex
  • Access protocol library
  • Available for PC laptops or desktop computers
  • Compatible with KingFisher Apex instruments only

How to install BindIx software*?

  1. Download BindIx here
  2. Download BindIx from Microsoft Store here
    BindIx from Microsoft Store can be installed by selecting “Install” button on MS Store.
  3. USB stick (delivered from manufacture)
    BindIx from USB stick can be installed by double-clicking .msix bundle file and after that selecting “Install” button.

Note: System requirements for BindIx software is Windows 10 version 1809 (17763.0) or higher.

DNA & RNA extraction protocols available to download by SKU

Nucleic acidSample typeKit nameSKU Format Software protocol
RNASalivaMagMAX Viral/Pathogen Kit (MVP I)A48310
A42352
up to 2,000 preps
up to 200 preps
MagMAX Viral/Pathogen RNA Saliva
Flex
MagMAX Viral/Pathogen II Kit (MVPII)A48383up to 2,000 preps
DNABlood, buccal, swabsMagMAX DNA Multi-Sample UltraA25597
A25598
500 preps
2,500 preps
MagMAX DNA Blood Buccal
 Duo Prime Flex
DNABlood, buccal, swabsMagMAX DNA Multi-Sample Ultra w/ extra PK and beadsA25919
A25920
500 preps
2,500 preps
MagMAX DNA Blood Buccal
Duo PrimeFlex
DNABlood, buccal, swabsMagMAX DNA Multi-Sample441302050 prepsMagMAX DNA Blood Buccal
Duo PrimeFlex
DNABlood, buccal, swabsMagMAX-96 DNA Multi-Sample4413021
4413022
96 preps
5 x 96 preps
MagMAX DNA Blood Buccal
 Duo PrimeFlex
DNAPCR reactionChargeSwitch PCR Clean-Up KitCS12000
CS1200010
100 preps
960 preps
ChargeSwitch PCR reaction
 Flex
DNATissue, cellsMagMAX DNA Multi-Sample UltraA25597
A25598
500 preps
2,500 preps
MagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX DNA Multi-Sample Ultra w/ extra PK and beadsA25919
A25920
500 preps
2,500 preps
MagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX DNA Multi-Sample441302150 prepsMagMAX DNA tissue cells
Duo PrimeFlex
DNATissue, cellsMagMAX-96 DNA Multi-Sample4413021
4413022
96 preps
5 x 96 preps
MagMAX DNA tissue cells
Duo PrimeFlex
RNA/DNAViralMagMAX Pathogen RNA/DNA4462359480 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Viral RNAAM183696 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Total Nucleic AcidAM1840100 prepsMagMAX Pathogen Std Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Pathogen RNA/DNA4462359480 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Viral RNAAM183696 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNA/DNAViralMagMAX Total Nucleic AcidAM1840100 prepsMagMAX Pathogen High Volume
Duo PrimeFlex
RNAViralMagMAX-96 AI/ND Viral RNA Isolation KitAM1835384 prepsMagMAX AI/ND Viral RNA
Duo PrimeFlex
RNA/
microRNA
Tissue, cells
blood, plasma, urine
MagMAX mirVana Total RNAA2782896 prepsMagMAX mirVana tissue cells
Duo PrimeFlex

MagMAX mirVana biofluids
Duo PrimeFlex
DNAPlasmaMagMAX Cell-Free DNAA2931996 prepsMagMAX Cell-Free DNA 2mL plasma
Duo PrimeFlex

MagMAX Cell-Free DNA 4mL plasma
Duo PrimeFlex
RNATissue, cells, plantsMagMAX-96 Total RNAAM183096 prepsMagMAX Total RNA tissue cell plant
Duo PrimeFlex
RNABloodMagMAX for Stabilized Blood (Paxgene tubes)445189496 prepsMagMAX for Paxgene tubes
Duo PrimeFlex
RNABloodMagMAX for Stabilized Blood (Tempus tubes)445189396 prepsMagMAX for Tempus tubes
Duo PrimeFlex
DNALeukocytesMagMAX DNA Multi-Sample UltraA25597500 prepsMagMAX DNA leukocytes
Duo PrimeFlex
mRNA Dynabeads mRNA Direct61011
61012
5 mL
10 mL
Dynabeads mRNA from crude samples
Duo PrimeFlex
RNA MagMAX for Microarrays Total RNA IsolationAM183996 prepsMagMAX for Microarrays with 100uL aqueous phase
Duo PrimeFlex
DNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra DNA only
Duo PrimeFlex
RNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra RNA only
Duo PrimeFlex
RNA/DNAFFPEMagMAX FFPE DNA/RNA Ultra KitA318811 kitMagMAX FFPE Ultra DNA and RNA
Duo PrimeFlex

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Uploadand find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Immunoprecipitation protocols for KingFisher Flex or DUO Prime automation

† Strategy (Indirect or Direct). This refers to the order of binding steps, and it primarily pertains to immunoprecipitation protocols. Indirect protocols involve incubating the target-containing sample and antibody first, and then incubating with the beads second. Direct protocols involve incubating beads and antibody first (either to bind or covalently couple the IP antibody), and then incubating the bead-antibody complex with the sample.

Ligand Product Protocol name: description Strategy† Download file
AntibodiesFabRICATOR MagIC immobilized on magnetic beadsHigh throughput parallel analysis of antibodiesIndirectFlex
Duo Prime
Protein ADynabeads Protein AIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein ADynabeads Protein AIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein ADynabeads Protein A Immunoprecipitation KitIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein ADynabeads Protein A Immunoprecipitation KitIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein GDynabeads Protein GIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein GDynabeads Protein GIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Protein GDynabeads Protein G Immunoprecipitation KitIP Dynabeads M-280 Protein A G Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Protein GDynabeads Protein G Immunoprecipitation KitIP Dynabeads M-280 Protein A G Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Duo Prime
Sheep anti-Mouse IgGDynabeads Sheep anti-Mouse IgGIP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
Sheep anti-Rabbit IgGDynabeads Sheep anti-Rabbit IgGIP Dynabeads M-280 SAM SAR Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
StreptavidinDynabeads M-280 StreptavidinIP Dynabeads M-280 SA Direct:
Immunoprecipitation (IP or Co-IP)
DirectFlex
Duo Prime
GlutathionePierce Glutathione Magnetic Agarose BeadsPurify GST tag Magnetic Agarose GSH Direct:
GST fusion protein purification
DirectFlex
Duo Prime
Ni-IMACPierce High Capacity Ni-IMAC MagBeads, EDTA-compatiblePurify His tag Magnetic Agarose Ni-IMAC DirectDirectFlex
Duo Prime
Ni-NTAPierce Ni-NTA Magnetic Agarose BeadsPurify His tag Magnetic Agarose Ni-NTA Direct:
His-tagged fusion protein purification
DirectFlex
Duo Prime
Protein A/GPierce Protein A/G Magnetic Agarose BeadsPurify Ab Magnetic Agarose Protein AG Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Duo Prime
Anti-HA AntibodyPierce Anti-HA Magnetic BeadsIP HA tag Pierce anti-HA Ab Direct:
HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
DirectFlex
Anti-c-Myc AntibodyPierce Anti-c-Myc Magnetic BeadsIP Myc tag Pierce anti-Myc Ab Direct:
Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP)
DirectFlex
GlutathionePierce Glutathione Magnetic BeadsPurify GST tag Pierce GSH Direct:
GST fusion protein purification or pull-down
DirectFlex
Ni-NTAHisPur Ni-NTA Magnetic BeadsPurify His tag Pierce Ni-NTA Direct:
His-tagged fusion protein purification or pull-down
DirectFlex
NHS EsterPierce NHS-Activated Magnetic BeadsCouple Pierce NHS ester Direct:
Coupling (immobilization) of primary amine molecule (protein, peptide)
DirectFlex
NHS EsterPierce NHS-Activated Magnetic BeadsIP Pierce NHS ester Direct:
Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide)
DirectFlex
Protein A/GPierce Protein A/G Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein APierce Protein A Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein GPierce Protein G Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of IgG containing Fc region
DirectFlex
Protein LPierce Protein L Magnetic BeadsPurify Ab Pierce Protein AGL Direct:
Antibody purification of immunoglobulins containing kappa light chains
DirectFlex
Protein A/GPierce Protein A/G Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein APierce Protein A Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein GPierce Protein G Magnetic BeadsIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
Protein A/GPierce Classic Magnetic IP/Co-IP KitIP Pierce Protein AGL Indirect:
Immunoprecipitation (IP or Co-IP)
IndirectFlex
NHS EsterPierce Direct Magnetic IP/Co-IP KitIP Pierce Direct IP Kit Direct:
Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads
DirectFlex
Protein A/GPierce Crosslink Magnetic IP/Co-IP KitIP Pierce Crosslink IP Kit Direct:
Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads
DirectFlex
Protein A/GPierce Magnetic ChIP KitChIP Pierce ChIP Kit Indirect:
Chromatin immunoprecipition (ChIP) of protein-DNA complexes
IndirectFlex
StreptavidinPierce Streptavidin Magnetic BeadsIP Pierce SA heated elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution
IndirectFlex
StreptavidinPierce Streptavidin Magnetic BeadsIP Pierce SA low pH elution Indirect:
Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution
IndirectFlex
Titanium Dioxide (TiO2)Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment KitEnrich pPep Pierce pPep Kit Direct deepwell plate:
Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
DirectFlex
Titanium Dioxide (TiO2)Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment KitEnrich pPep Pierce pPep Kit Direct PCR plate:
Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine)
DirectFlex

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connect in BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Upload and find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.

Dynabeads virus enrichment and positive cell isolation protocols to download

LigandProductProtocol descriptionStrategyDownload file
VirusViruses (especially SARS-CoV-2), vesicles and proteins Dynabeads Intact Virus EnrichmentIndirectDuo Prime
VirusViruses (especially SARS-CoV-2), vesicles and proteinsDynabeads Intact Virus EnrichmentIndirectFlex
CellsCD3, CD4, CD8, CD14, CD19 Positive Cell Isolation for downstream nucleic acid,  protein analysis, or cell depletionIndirectDuo Prime
CellsCD3, CD4, CD8 Positive Cell Isolation for downstream cellular applications – such as T-cell ActivationIndirectDuo Prime

Instructions for downloading and installing protocols:

  1. Make sure you have BindIt 4.1 Software installed in your computer
  2. Click the protocol in the table. Protocol opens directly in BindIt Software
  3. Save the protocol in your computer in the preferred folder.
  4. Connect your PC with USB cable to KingFisher instrument and select Home > Connectin BindIt Software.
  5. You can start the protocol from the BindIt or transfer it into instrument. If you transfer the protocol select Home > Transfer in BindIt Software.
  6. Select Uploadand find the saved protocol. Click open.
  7. Select the folder (category) you want to store the protocol in the instrument.
  8. Your protocol can be now found in the instrument memory.
  9. Disconnect your instrument from BindIt and check using keypad that you protocol is in the desired folder.