KingFisher Duo Prime

The Thermo Scientific KingFisher Duo Prime is the most versatile compact benchtop sample preparation instrument in the lab for 6 or 12 samples per run. It offers consistent extraction and purification of DNA, RNA, proteins, and cells, and includes an ultraviolet lamp for decontamination. The KingFisher Duo Prime is an affordable choice for academic or small labs.


In addition to the common features of all KingFisher Purification Systems, the Duo Prime offers:

Automated purification

6–12 samples per run


Choose from two plate formats

Wide 20 μL–5 mL volume range


Download protocols from our library

Or easily customize protocols using ThermoScientific BindIt Software

Easy to install and run

Ready to run in 10 minutes or less


Simple workflow

Just follow the on-screen instructions


Ultraviolet lamp

Decontamination is standard

Demo videos


The KingFisher Duo Prime can gently and efficiently isolate and purify nucleic acids, proteins, and cells from a range of sample types for a wide variety of downstream applications. For a selection of application kits, reagents, and protocols that run on the KingFisher Duo Prime, see the Kits, Reagents, and Protocols tab.

Protocols and software

Thermo Scientific BindIt software is included with KingFisher Duo Prime and Flex instruments. The software makes it easy to create, download, run, modify, and store protocols for your KingFisher applications.


Protocols are available for nucleic acid and protein purification procedures with reagent kits. The software allows you to define and edit steps, parameters, plates, and reagents for every part of your protocol—bind, wash, and elute.


For more information about BindIt, see the Features tab.

Plates, plastics, and setup

For information about plates, plastics, and plate setup products designed to work with the KingFisher Duo Prime, see the Accessories tab.




Instrument size

Compact benchtop

Throughput level

Low to medium

Processing volume range

50–1,000 µL: 12-pin magnet head
200–5,000 µL: 6-pin magnet head

Samples per run

6 or 12

Software included


Customizable protocols

Yes (with BindIt software)


10ºC–75ºC (plate row block A)
4ºC–75ºC (elution strip block)

Ultraviolet lamp

8 watts (up to 16 hr)

Bar code reader

With BindIt software or standalone

User-selectable magnetic heads


Standalone use



40 x 46 x 34 cm; 15.7 x 18.1 x 13.4 in. (W x D x H)


17 kg; 37.5 lbs

Ordering information