Large-scale plasmid isolation in mega and giga formats
Invitrogen™ PureLink™ HiPure Expi Plasmid Purification Kits use a novel, proprietary anion-exchange resin to purify the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid isolations are typically completed in around 90 minutes, with purity equal to that obtained with cesium chloride (CsCl) gradients. PureLink HiPure Expi Plasmid Purification Kits are offered in both mega and giga formats, providing you the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.
PureLink HiPure Expi Plasmid Megaprep Kit
The PureLink HiPure Expi Plasmid Megaprep Kit includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The kit typically isolates 4 mg of high-quality, ultrapure plasmid DNA with inherently low endotoxin levels from 500 mL of bacterial culture. Advantages of the kit include:
- High yield–isolate over 5 mg of high-quality plasmid DNA from a single purification using 1 L of bacterial culture
- Purity–inherently low endotoxin levels (typically 0.1–1.0 EU/µg), and A260/A280 >1.8, making it ideal for mammalian cell transfection
- Simplicity and speed–with the vacuum-assisted protocol, typical plasmid isolation takes only 90 minutes to complete