Kits for DNA gel extraction, cell-free DNA isolation, PCR clean-up, and microbiome DNA extraction
Whether you prefer organic reagents, filter columns, or magnetic beads, our products for Isolation of DNA fragments are designed for sensitive, scalable extraction and are compatible with a range of downstream applications.
Looking to isolate genomic DNA? Check out genomic DNA extraction by sample type
PCR purification kits
PCR clean-up is a routine but critical laboratory procedure. You need a PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.
Invitrogen DNA purification kits provide easier, faster, and safer methods for isolation of DNA fragments that yield superior results. Purified DNA fragments are ready for sequencing, additional PCR, transcription, mapping, cloning, and labeling.
Learn more about purifying your PCR product with PureLink PCR cleanup kits
See how the competitors stack up: Compare efficiency of PCR cleanup kits
Which PCR purification kit is right for you?
| Quick and efficient removal of by-products | Concentrates low-yield PCR products | Simple, reliable, rapid method, 96-well format | Rapid, scalable, magnetic bead format | |
|---|---|---|---|---|
| Product name | PureLink PCR Purification Kit | PureLink PCR Micro Purification Kit | PureLink 96 PCR Purification Kit | ChargeSwitch PCR Clean-Up Kit |
| Format | Silica column | Silica column | 96-well silica filter plate | Magnetic beads |
| Prep time | 15 mins | 15 mins | 15 mins | 5 mins |
| Sample input volume | 50–100 µL | 50–100 µL | 50–100 µL | 25–50 µL |
| DNA fragment size | 100 bp to 15 kb | 100 bp to 15 kb | 100 bp to 15 kb | 90 bp to 40 kb |
| DNA binding capacity | Up to 40 µg | Up to 40 µg | Up to 40 µg | ~25 µg per mg beads |
| Downstream applications | Sequencing, NGS, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Cloning | Sequencing, NGS, Microarray Analysis, PCR, Cloning |
| Automation compatibility | No | No | Yes | Yes |
| Pack size | 50 preps 250 preps | 50 preps 250 preps | 4 plates | 100 preps 960 preps |
| Cat. No. | K310001 K310002 | K310050 K310250 | K3100-96A | CS12000 CS12000-10 |
Perform both PCR purification and gel extraction?
The Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit contains everything you need for both procedures.
Cell-free DNA isolation
Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA) and viruses. Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples.
Large volumes of biological fluids are sometimes required to obtain sufficient amounts of cfDNA for analysis. Invitrogen/Thermo Fisher Scientific offers scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types.
Which cell-free DNA isolation kit is right for you?
| Fast isolation of viral nucleic acid | Easy-to-use, high sensitivity | Automated format, capable of processing large volumes of plasma | |
|---|---|---|---|
| Product name | PureLink Viral RNA/DNA Mini Kit | PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Cell-Free DNA Isolation Kit |
| Sample input volume | 500 µL | 200 µL | 0.5–10 mL |
| Compatible sample types | Plasma, serum, cerebrospinal fluid | Plasma, serum, cerebrospinal fluid | Plasma, serum, urine |
| Isolation technology | Silica spin column | Silica microplate | Paramagnetic beads |
| Prep time | 15 mins | 35 mins | 24 samples in <1 hr |
| High-throughput compatibility | No | Yes | Yes |
| Downstream applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
| Prep size | 50 preps | 4 x 96 preps | 50 preps |
Quick and easy DNA gel extraction
Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. High recovery of DNA fragments is critical to the success of the entire workflow.
Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15–30 minutes. The silica membrane technology captures DNA fragments without the need for pH adjustment.
With Invitrogen kits, DNA isolated from agarose gel is free of proteins, dye, and agarose; it is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.
Which DNA gel extraction kit is right for you?
| Environmentally friendly | High DNA binding capacity | Yields highly concentrated DNA | |
|---|---|---|---|
| Product name | PureLink Quick Gel Extraction Kit | GeneJET Gel Extraction Kit | GeneJET Gel Extraction and DNA Cleanup Micro Kit |
| DNA binding capacity | 15 µg per column | 25 µg per column | 10 µg per column |
| Isolation technology & format | Silica spin/vacuum column | Silica spin/vacuum column | Silica spin/vacuum column |
| Prep time | 30 mins | 15 mins | 15 mins |
| DNA fragment size range | 40 bp to 10 kb | 100 bp to 20 kb | 25 bp to 20 kb |
| Elution volume | 50 µL | 20 to 50 µL | 6 to 10 µL |
| Automation compatibility | No | No | No |
| Input sample types | Agarose gel slices | Agarose gel slices | Agarose gel slices, PCR and other enzymatic reaction mixtures |
Figure 1. Amplification of DNA isolated using the PureLink Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR Safe DNA Gel Stain.
See how the competitors stack up: Comparison of DNA gel extraction kits
Green benefits of the PureLink Quick Gel Extraction Kit 
Microbiome DNA purification Kit
The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil.
The kit uses proven PureLink spin column technology, combined with efficient triple-lysis approach and fast removal of inhibitors, to provide excellent yields of purified DNA from bacteria or fungi as well as host DNA.
The versatility of this microbiome DNA purification procedure makes it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.
Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples
Medical Science Review: The human microbiota: composition, functions, and therapeutic potential
The PureLink Microbiome DNA Purification Kit offers:
- Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
- Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
- Streamlined protocols for a variety of biological samples
- Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
One kit to isolate microbial and host DNA from diverse sample types
The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:
- Stool
- Urine
- Saliva
- Soil
- Swabs (vaginal, buccal, skin, rectal, environmental)
- Transport media
- Growth media
PCR purification kits
PCR clean-up is a routine but critical laboratory procedure. You need a PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.
Invitrogen DNA purification kits provide easier, faster, and safer methods for isolation of DNA fragments that yield superior results. Purified DNA fragments are ready for sequencing, additional PCR, transcription, mapping, cloning, and labeling.
Learn more about purifying your PCR product with PureLink PCR cleanup kits
See how the competitors stack up: Compare efficiency of PCR cleanup kits
Which PCR purification kit is right for you?
| Quick and efficient removal of by-products | Concentrates low-yield PCR products | Simple, reliable, rapid method, 96-well format | Rapid, scalable, magnetic bead format | |
|---|---|---|---|---|
| Product name | PureLink PCR Purification Kit | PureLink PCR Micro Purification Kit | PureLink 96 PCR Purification Kit | ChargeSwitch PCR Clean-Up Kit |
| Format | Silica column | Silica column | 96-well silica filter plate | Magnetic beads |
| Prep time | 15 mins | 15 mins | 15 mins | 5 mins |
| Sample input volume | 50–100 µL | 50–100 µL | 50–100 µL | 25–50 µL |
| DNA fragment size | 100 bp to 15 kb | 100 bp to 15 kb | 100 bp to 15 kb | 90 bp to 40 kb |
| DNA binding capacity | Up to 40 µg | Up to 40 µg | Up to 40 µg | ~25 µg per mg beads |
| Downstream applications | Sequencing, NGS, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Cloning | Sequencing, NGS, Microarray Analysis, PCR, Cloning |
| Automation compatibility | No | No | Yes | Yes |
| Pack size | 50 preps 250 preps | 50 preps 250 preps | 4 plates | 100 preps 960 preps |
| Cat. No. | K310001 K310002 | K310050 K310250 | K3100-96A | CS12000 CS12000-10 |
Perform both PCR purification and gel extraction?
The Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit contains everything you need for both procedures.
Cell-free DNA isolation
Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA) and viruses. Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples.
Large volumes of biological fluids are sometimes required to obtain sufficient amounts of cfDNA for analysis. Invitrogen/Thermo Fisher Scientific offers scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types.
Which cell-free DNA isolation kit is right for you?
| Fast isolation of viral nucleic acid | Easy-to-use, high sensitivity | Automated format, capable of processing large volumes of plasma | |
|---|---|---|---|
| Product name | PureLink Viral RNA/DNA Mini Kit | PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Cell-Free DNA Isolation Kit |
| Sample input volume | 500 µL | 200 µL | 0.5–10 mL |
| Compatible sample types | Plasma, serum, cerebrospinal fluid | Plasma, serum, cerebrospinal fluid | Plasma, serum, urine |
| Isolation technology | Silica spin column | Silica microplate | Paramagnetic beads |
| Prep time | 15 mins | 35 mins | 24 samples in <1 hr |
| High-throughput compatibility | No | Yes | Yes |
| Downstream applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
| Prep size | 50 preps | 4 x 96 preps | 50 preps |
Quick and easy DNA gel extraction
Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. High recovery of DNA fragments is critical to the success of the entire workflow.
Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15–30 minutes. The silica membrane technology captures DNA fragments without the need for pH adjustment.
With Invitrogen kits, DNA isolated from agarose gel is free of proteins, dye, and agarose; it is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.
Which DNA gel extraction kit is right for you?
| Environmentally friendly | High DNA binding capacity | Yields highly concentrated DNA | |
|---|---|---|---|
| Product name | PureLink Quick Gel Extraction Kit | GeneJET Gel Extraction Kit | GeneJET Gel Extraction and DNA Cleanup Micro Kit |
| DNA binding capacity | 15 µg per column | 25 µg per column | 10 µg per column |
| Isolation technology & format | Silica spin/vacuum column | Silica spin/vacuum column | Silica spin/vacuum column |
| Prep time | 30 mins | 15 mins | 15 mins |
| DNA fragment size range | 40 bp to 10 kb | 100 bp to 20 kb | 25 bp to 20 kb |
| Elution volume | 50 µL | 20 to 50 µL | 6 to 10 µL |
| Automation compatibility | No | No | No |
| Input sample types | Agarose gel slices | Agarose gel slices | Agarose gel slices, PCR and other enzymatic reaction mixtures |
Figure 1. Amplification of DNA isolated using the PureLink Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR Safe DNA Gel Stain.
See how the competitors stack up: Comparison of DNA gel extraction kits
Green benefits of the PureLink Quick Gel Extraction Kit
Microbiome DNA purification Kit
The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil.
The kit uses proven PureLink spin column technology, combined with efficient triple-lysis approach and fast removal of inhibitors, to provide excellent yields of purified DNA from bacteria or fungi as well as host DNA.
The versatility of this microbiome DNA purification procedure makes it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.
Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples
Medical Science Review: The human microbiota: composition, functions, and therapeutic potential
The PureLink Microbiome DNA Purification Kit offers:
- Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
- Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
- Streamlined protocols for a variety of biological samples
- Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
One kit to isolate microbial and host DNA from diverse sample types
The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:
- Stool
- Urine
- Saliva
- Soil
- Swabs (vaginal, buccal, skin, rectal, environmental)
- Transport media
- Growth media
Resources and support for PCR cleanup, DNA gel extraction, and cell-free DNA isolation
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
- Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
- Videos—View our library of DNA & RNA purification and analysis videos.
- Application Notes—Explore our application notes from scientists sharing data for isolation products
For Research Use Only. Not for use in diagnostic procedures.
