A full suite of products for all your DNA extraction needs
Our DNA extraction products include kits and reagents for the purification of cell-free DNA, microbial DNA, PCR Clean-up, and sequence-specific DNA.
Learn more about DNA extraction products for:
DNA extraction from cell-free samples
Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA). cfDNA is used in pathogen detection, and cfDNA targets are being used as biomarkers in oncology research. Isolation of cfDNA is challenging because of its low abundance in typical samples. In pathogen detection, virus particles are also typically in low abundance and therefore are challenging to capture. Thus, large volumes of biological fluids (which can be difficult to handle) are required to obtain sufficient amounts of cfDNA for analysis. We have developed scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types in several format options.
Which DNA extraction kit for plasma, serum, and urine samples is right for you?
| Order now | Order now | Order now |
|
| Fast isolation of viral nucleic acid | Easy-to-use, high-sensitivity | Automated format, capable of processing large volumes of plasma | |
|---|---|---|---|
| PureLink Viral RNA/DNA Mini Kit | PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Cell-Free DNA Isolation Kit | |
| Sample input | 500 µL | 200 µL | 0.5–10 mL |
| Compatible samples | Plasma, serum, cerebrospinal fluid | Plasma, serum, cerebrospinal fluid | Plasma, serum, urine |
| Isolation method | Silica membrane | Filter plate | Magnetic beads |
| High-throughput compatible | No | Yes | Yes |
| Compatible applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
| Prep time | 15 min | 35 min | 24 samples in <1 hr |
| Prep size | 50 preps | 4 x 96 preps | 50 preps |
PureLink Microbiome DNA Purification Kit
The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. The kit uses proven PureLink spin column technology for robust yields of purified DNA from bacteria or fungi, ready for downstream applications such as PCR and sequencing. The highly efficient triple-lysis approach, fast removal of inhibitors, and versatility of this DNA extraction procedure make it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.
The PureLink Microbiome DNA Purification Kit offers:
- Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
- Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
- Streamlined protocols for a variety of biological samples
- Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
One kit to isolate microbial and host DNA from a diversity of sample types
The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:
|
|
Video: How to purify microbial and host DNA from stool samples
Learn how to isolate microbial DNA that accurately reflects the diverse microbes in the community sampled. This video will provide an outline of stool microbial DNA isolation, plus some tips and tricks. In addition to stool, the PureLink Microbiome DNA Purification Kit can be used to isolate DNA from urine, saliva, swabs, transport media, microbial culture, and soil.
View all PureLink Microbiome DNA Purification Kit product user guides
Video: Bringing bacteria out of hiding: Understanding the microbiome.
Dr. Watts, the co-director of the Genomics Shared Service at the University of Arizona Cancer Center, focuses on understanding the human microbiome and its role in disease onset and progression. In particular, they are employing 16S RNA sequencing to help correctly identify all of the bacteria present in individuals with diabetic foot ulcers.
Supporting experimental data
Figure 2. Purification of bacterial DNA from culture media. E. coli DNA was isolated from 0.2, 0.4, and 1 mL culture samples (in triplicate) with the PureLink Microbiome DNA Purification Kit.
(A) Concentrations of DNA as measured by a Thermo Scientific NanoDrop spectrophotometer (blue bars) and Invitrogen Qubit fluorometer (red bars) are shown. For all preparations DNA was highly pure: A260/A280 = 1.9, A260/A230 = 2.1–2.3.
(B) qPCR analysis with an E. coli–specific TaqMan assay. Threshold cycles (Ct) are displayed for isolations performed with three sample input volumes.
User guides
PCR clean-up from complex mixtures of DNA
PCR clean-up is a routine but time-consuming laboratory procedure. Now you can use an easier, faster, safer method and get superior results. Purify DNA using a simple and rapid PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.
Isolated DNA is ready for sequencing, PCR, transcription, mapping, cloning, and labeling. We offer a wide range of Invitrogen PCR clean-up kits, plus the support you may need to get high yields of pure DNA.
Which PCR product clean-up kit is right for you?
| Order now | Order now | Order now | Order now | |
| Quick and Efficient removal of by-products | Ideal for concentrating low yield PCR products | Simple, reliable, rapid method, 96-well format | Rapid, scalable, magnetic bead format | |
|---|---|---|---|---|
| PureLink PCR Purification Kit | PureLink PCR Micro Purification Kit | PureLink 96 PCR Purification Kit | ChargeSwitch PCR Clean-Up Kit | |
| Format | Spin/vacuum column | Spin/vacuum column | 96-well plate (vacuum/centrifuge) | Magnetic beads |
| Protocol time | 15 min | 15 min | 15 min | 5 min |
| Sample volume | 50–100 µL | 50–100 µL | 50–100 µL | 25–50 µL |
| Yield plasmid DNA | Up to 40 µg | Up to 20 µg | Up to 20 µg | Up to 30 µg |
| DNA fragment size | 100 bp–15 kb | 100 bp–15 kb | 100 bp–15 kb | 90 bp–40 kb |
| Binding capacity | Up to 40 µg | Up to 40 µg | Up to 40 µg | ~25 µg per mg beads |
| Downstream Application | Sequencing, Sequencing (Next Generation), Nucleic Acid Labeling, PCR, Cloning | Sequencing, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Cloning | Sequencing, Sequencing (Next Generation), Microarray Analysis, PCR, Cloning |
| High Throughput Compatibility | No | No | Yes | Yes |
| Pack size | 50 preps 250 preps | 50 preps 250 preps | 4 plates | 100 preps 960 preps |
| Cat. No. | K310001 K310002 |
K310050 K310250 |
K3100-96A | CS12000 CS12000-10 |
Perform both PCR purification and gel extraction?
If so, consider our Invitrogen PureLink combo kit that allows you to do either process with a single kit.
Learn more about the Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit
Quick and easy gel extraction of DNA
The PureLink Quick Gel Extraction Kit is designed to purify DNA fragments from agarose gels in less than 30 minutes. The simple procedure uses a unique silica-membrane spin column to capture and purify DNA fragments from 40 bp to 10 kb, without the need for pH adjustment. Isolated DNA is free of proteins, dye, and agarose and is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling (Figure 1).
Green benefits of the PureLink Quick Gel Extraction Kit 
- Less hazardous
- Less use of nonrenewable resources
- Less energy to produce
- Decreased fuel consumption and greenhouse gas emissions for transport
- Less waste disposal
PureLink Combo Kit
Isolating DNA from complex PCR mixtures and recovering bands from agarose gels? Try our combo kit
The PureLink Quick Gel Extraction and PCR Purification Combo Kit offers the ability to perform both gel extraction and PCR purification in a single kit.
Learn more about the PureLink Quick Gel Extraction and PCR Purification Combo Kit ›
Technical Resources
|
Sequence-Specific RNA/DNA Purification
Invitrogen streptavidin-coupled Dynabeads are a robust and versatile tool that can be used to capture specific RNA or DNA sequences and then pull them directly out of solution. These monosized superparamagnetic Dynabeads provide an efficient and solid-phase alternative to nitrocellulose and provide you with an unmatched level of product quality and data consistency. Excellent near–liquid phase reaction kinetics allow for extremely fast protocols. The inherent ease of magnetic handling means that downstream manipulations and buffer changes are as simple as concentrating the bead-bound target at the tube-wall with a magnet and then discarding the supernatant. These beads are compatible with an extremely broad range of sample types including most bodily fluids, crude lysates of plant, animal and microbial origin as well as purified total RNA or DNA. Since these Dynabeads will only interact with specifically targeted RNA or DNA molecules, upstream purification of total RNA or DNA is almost always an unnecessary step.
The direct capture procedure involves the immobilization of double-stranded PCR products onto the beads. These are easily converted to single-stranded bead-bound templates which are then used to capture specific RNA or DNA molecules directly from solution.
An alternative indirect capture approach will offer faster reaction kinetics in some cases. This indirect capture procedure allows the target sequence to be captured prior to being immobilized the magnetic beads. First, a biotinylated capture-sequence (single-stranded DNA) is incubated with the sample and allowed to hybridize to the targeted RNA or DNA molecules in solution. Streptavidin-coated Dynabeads are then added to the mixture and the hybridized sequences are immobilized onto the Dynabeads via the streptavidin-biotin bond.
The 1 µm Dynabeads MyOne Streptavidin C1 present a very high surface area per mg of beads, enabling high enrichment of low abundance RNA or DNA. When the goal is to capture nucleic acid from more viscous samples such as cerebrospinal fluid, the larger 2.8 µm sized Dynabeads M-270 Streptavidin are recommended. These Dynabeads (MyOne Streptavidin C1 and M-270 Streptavidin) are optimally designed to have slightly negatively charged surfaces which ensure negligible non-specific binding of non-target nucleic acid sequences.
Examples of applications include; isolation of RNA/DNA infectious agent (1,2,3,4), subtractive hybridization (5,6,7), cDNA selection and enrichment, detection and isolation of mutated sequences (8,9,10), isolation of cell specific transcripts and mRNA differential display.
Learn more about Nucleic Acid Capture Assays ›
- Meng Q. et al. (2001) Automated multiplex assay system for simultaneous detection of hepatitis B virus DNA, hepatitis C virus RNA and human immunodeficiency virus type 1 RNA. J.Clin.Microbiol. 39(8):2937-2945.
- Stevens SJC. et al. (1999) Monitoring of Epstein-Barr virus DNA load in peripheral blood by quantitative competitive PCR. J.Clin. Microbiol. 37:2852-2857.
- Mangiapan G. et al. (1996) Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens. J. Clin. Microbiol. 34(5):1209-1215.
- Shuber AP. et.al. (2002) Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment. J. Clin. Microbiol. 40(1):262-264.
- Hansen-Hagge TE. et.al. (2001) Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (LIMES). Nucl. Acids Res. 29(4):e20.
- Pradel N. et.al. (2002) Genomic subtraction to identify and characterize sequences of Shiga toxin-producing Escherichia coli O91:H21. Appl. Env. Microbiol. 68(5):2316-2325.
- Laveder P. et.al. (2002) A two-step strategy for constructing specifically self-subtracted cDNA libraries. Nucleic Acids Res. 30(9):e38.
- Lindblad-Toh K.et.al. (2000) Large-scale discovery and genotyping of single-nucleotide polymorphisms in the mouse. Nature Genetics. 24:381-386.
- Miyashiro I. et.al. (2001) Molecular strategy for detecting metastatic cancers with use of multiple tumor-specific MAGE-A genes. Clin.Chem. 47(3):505-512.
- Dong SM. et.al. (2001) Detection of colorectal cancer in stool with the use of multiple genetic targets. J Natl. Cancer Inst. 93(11):858-865.
DNA extraction from cell-free samples
Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA). cfDNA is used in pathogen detection, and cfDNA targets are being used as biomarkers in oncology research. Isolation of cfDNA is challenging because of its low abundance in typical samples. In pathogen detection, virus particles are also typically in low abundance and therefore are challenging to capture. Thus, large volumes of biological fluids (which can be difficult to handle) are required to obtain sufficient amounts of cfDNA for analysis. We have developed scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types in several format options.
Which DNA extraction kit for plasma, serum, and urine samples is right for you?
| Order now | Order now | Order now |
|
| Fast isolation of viral nucleic acid | Easy-to-use, high-sensitivity | Automated format, capable of processing large volumes of plasma | |
|---|---|---|---|
| PureLink Viral RNA/DNA Mini Kit | PureLink Pro 96 Viral RNA/DNA Purification Kit | MagMAX Cell-Free DNA Isolation Kit | |
| Sample input | 500 µL | 200 µL | 0.5–10 mL |
| Compatible samples | Plasma, serum, cerebrospinal fluid | Plasma, serum, cerebrospinal fluid | Plasma, serum, urine |
| Isolation method | Silica membrane | Filter plate | Magnetic beads |
| High-throughput compatible | No | Yes | Yes |
| Compatible applications | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping | Cloning, qPCR, sequencing, genotyping |
| Prep time | 15 min | 35 min | 24 samples in <1 hr |
| Prep size | 50 preps | 4 x 96 preps | 50 preps |
PureLink Microbiome DNA Purification Kit
The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil. The kit uses proven PureLink spin column technology for robust yields of purified DNA from bacteria or fungi, ready for downstream applications such as PCR and sequencing. The highly efficient triple-lysis approach, fast removal of inhibitors, and versatility of this DNA extraction procedure make it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.
The PureLink Microbiome DNA Purification Kit offers:
- Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
- Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
- Streamlined protocols for a variety of biological samples
- Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
One kit to isolate microbial and host DNA from a diversity of sample types
The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:
|
|
Video: How to purify microbial and host DNA from stool samples
Learn how to isolate microbial DNA that accurately reflects the diverse microbes in the community sampled. This video will provide an outline of stool microbial DNA isolation, plus some tips and tricks. In addition to stool, the PureLink Microbiome DNA Purification Kit can be used to isolate DNA from urine, saliva, swabs, transport media, microbial culture, and soil.
View all PureLink Microbiome DNA Purification Kit product user guides
Video: Bringing bacteria out of hiding: Understanding the microbiome.
Dr. Watts, the co-director of the Genomics Shared Service at the University of Arizona Cancer Center, focuses on understanding the human microbiome and its role in disease onset and progression. In particular, they are employing 16S RNA sequencing to help correctly identify all of the bacteria present in individuals with diabetic foot ulcers.
Supporting experimental data
Figure 2. Purification of bacterial DNA from culture media. E. coli DNA was isolated from 0.2, 0.4, and 1 mL culture samples (in triplicate) with the PureLink Microbiome DNA Purification Kit.
(A) Concentrations of DNA as measured by a Thermo Scientific NanoDrop spectrophotometer (blue bars) and Invitrogen Qubit fluorometer (red bars) are shown. For all preparations DNA was highly pure: A260/A280 = 1.9, A260/A230 = 2.1–2.3.
(B) qPCR analysis with an E. coli–specific TaqMan assay. Threshold cycles (Ct) are displayed for isolations performed with three sample input volumes.
User guides
PCR clean-up from complex mixtures of DNA
PCR clean-up is a routine but time-consuming laboratory procedure. Now you can use an easier, faster, safer method and get superior results. Purify DNA using a simple and rapid PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.
Isolated DNA is ready for sequencing, PCR, transcription, mapping, cloning, and labeling. We offer a wide range of Invitrogen PCR clean-up kits, plus the support you may need to get high yields of pure DNA.
Which PCR product clean-up kit is right for you?
| Order now | Order now | Order now | Order now | |
| Quick and Efficient removal of by-products | Ideal for concentrating low yield PCR products | Simple, reliable, rapid method, 96-well format | Rapid, scalable, magnetic bead format | |
|---|---|---|---|---|
| PureLink PCR Purification Kit | PureLink PCR Micro Purification Kit | PureLink 96 PCR Purification Kit | ChargeSwitch PCR Clean-Up Kit | |
| Format | Spin/vacuum column | Spin/vacuum column | 96-well plate (vacuum/centrifuge) | Magnetic beads |
| Protocol time | 15 min | 15 min | 15 min | 5 min |
| Sample volume | 50–100 µL | 50–100 µL | 50–100 µL | 25–50 µL |
| Yield plasmid DNA | Up to 40 µg | Up to 20 µg | Up to 20 µg | Up to 30 µg |
| DNA fragment size | 100 bp–15 kb | 100 bp–15 kb | 100 bp–15 kb | 90 bp–40 kb |
| Binding capacity | Up to 40 µg | Up to 40 µg | Up to 40 µg | ~25 µg per mg beads |
| Downstream Application | Sequencing, Sequencing (Next Generation), Nucleic Acid Labeling, PCR, Cloning | Sequencing, Nucleic Acid Labeling, PCR, Cloning | Sequencing, Cloning | Sequencing, Sequencing (Next Generation), Microarray Analysis, PCR, Cloning |
| High Throughput Compatibility | No | No | Yes | Yes |
| Pack size | 50 preps 250 preps | 50 preps 250 preps | 4 plates | 100 preps 960 preps |
| Cat. No. | K310001 K310002 |
K310050 K310250 |
K3100-96A | CS12000 CS12000-10 |
Perform both PCR purification and gel extraction?
If so, consider our Invitrogen PureLink combo kit that allows you to do either process with a single kit.
Learn more about the Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit
Quick and easy gel extraction of DNA
The PureLink Quick Gel Extraction Kit is designed to purify DNA fragments from agarose gels in less than 30 minutes. The simple procedure uses a unique silica-membrane spin column to capture and purify DNA fragments from 40 bp to 10 kb, without the need for pH adjustment. Isolated DNA is free of proteins, dye, and agarose and is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling (Figure 1).
Green benefits of the PureLink Quick Gel Extraction Kit
- Less hazardous
- Less use of nonrenewable resources
- Less energy to produce
- Decreased fuel consumption and greenhouse gas emissions for transport
- Less waste disposal
PureLink Combo Kit
Isolating DNA from complex PCR mixtures and recovering bands from agarose gels? Try our combo kit
The PureLink Quick Gel Extraction and PCR Purification Combo Kit offers the ability to perform both gel extraction and PCR purification in a single kit.
Learn more about the PureLink Quick Gel Extraction and PCR Purification Combo Kit ›
Technical Resources
|
Sequence-Specific RNA/DNA Purification
Invitrogen streptavidin-coupled Dynabeads are a robust and versatile tool that can be used to capture specific RNA or DNA sequences and then pull them directly out of solution. These monosized superparamagnetic Dynabeads provide an efficient and solid-phase alternative to nitrocellulose and provide you with an unmatched level of product quality and data consistency. Excellent near–liquid phase reaction kinetics allow for extremely fast protocols. The inherent ease of magnetic handling means that downstream manipulations and buffer changes are as simple as concentrating the bead-bound target at the tube-wall with a magnet and then discarding the supernatant. These beads are compatible with an extremely broad range of sample types including most bodily fluids, crude lysates of plant, animal and microbial origin as well as purified total RNA or DNA. Since these Dynabeads will only interact with specifically targeted RNA or DNA molecules, upstream purification of total RNA or DNA is almost always an unnecessary step.
The direct capture procedure involves the immobilization of double-stranded PCR products onto the beads. These are easily converted to single-stranded bead-bound templates which are then used to capture specific RNA or DNA molecules directly from solution.
An alternative indirect capture approach will offer faster reaction kinetics in some cases. This indirect capture procedure allows the target sequence to be captured prior to being immobilized the magnetic beads. First, a biotinylated capture-sequence (single-stranded DNA) is incubated with the sample and allowed to hybridize to the targeted RNA or DNA molecules in solution. Streptavidin-coated Dynabeads are then added to the mixture and the hybridized sequences are immobilized onto the Dynabeads via the streptavidin-biotin bond.
The 1 µm Dynabeads MyOne Streptavidin C1 present a very high surface area per mg of beads, enabling high enrichment of low abundance RNA or DNA. When the goal is to capture nucleic acid from more viscous samples such as cerebrospinal fluid, the larger 2.8 µm sized Dynabeads M-270 Streptavidin are recommended. These Dynabeads (MyOne Streptavidin C1 and M-270 Streptavidin) are optimally designed to have slightly negatively charged surfaces which ensure negligible non-specific binding of non-target nucleic acid sequences.
Examples of applications include; isolation of RNA/DNA infectious agent (1,2,3,4), subtractive hybridization (5,6,7), cDNA selection and enrichment, detection and isolation of mutated sequences (8,9,10), isolation of cell specific transcripts and mRNA differential display.
Learn more about Nucleic Acid Capture Assays ›
- Meng Q. et al. (2001) Automated multiplex assay system for simultaneous detection of hepatitis B virus DNA, hepatitis C virus RNA and human immunodeficiency virus type 1 RNA. J.Clin.Microbiol. 39(8):2937-2945.
- Stevens SJC. et al. (1999) Monitoring of Epstein-Barr virus DNA load in peripheral blood by quantitative competitive PCR. J.Clin. Microbiol. 37:2852-2857.
- Mangiapan G. et al. (1996) Sequence capture-PCR improves detection of mycobacterial DNA in clinical specimens. J. Clin. Microbiol. 34(5):1209-1215.
- Shuber AP. et.al. (2002) Accurate, noninvasive detection of Helicobacter pylori DNA from stool samples: potential usefulness for monitoring treatment. J. Clin. Microbiol. 40(1):262-264.
- Hansen-Hagge TE. et.al. (2001) Identification of sample-specific sequences in mammalian cDNA and genomic DNA by the novel ligation-mediated subtraction (LIMES). Nucl. Acids Res. 29(4):e20.
- Pradel N. et.al. (2002) Genomic subtraction to identify and characterize sequences of Shiga toxin-producing Escherichia coli O91:H21. Appl. Env. Microbiol. 68(5):2316-2325.
- Laveder P. et.al. (2002) A two-step strategy for constructing specifically self-subtracted cDNA libraries. Nucleic Acids Res. 30(9):e38.
- Lindblad-Toh K.et.al. (2000) Large-scale discovery and genotyping of single-nucleotide polymorphisms in the mouse. Nature Genetics. 24:381-386.
- Miyashiro I. et.al. (2001) Molecular strategy for detecting metastatic cancers with use of multiple tumor-specific MAGE-A genes. Clin.Chem. 47(3):505-512.
- Dong SM. et.al. (2001) Detection of colorectal cancer in stool with the use of multiple genetic targets. J Natl. Cancer Inst. 93(11):858-865.
Compatability
Our products extract high-quality DNA with excellent reproducibility for many fundamental downstream molecular biology experiments, including:
- Next-generation Sequencing
- PCR
- DNA cloning
- DNA sequencing
- DNA electrophoresis
Resources
For Research Use Only. Not for use in diagnostic procedures.
