extracted genomic DNA

Kits for DNA gel extraction, cell-free DNA isolation, PCR clean-up, and microbiome DNA extraction

Whether you prefer organic reagents, filter columns, or magnetic beads, our products for Isolation of DNA fragments are designed for sensitive, scalable extraction and are compatible with a range of downstream applications.

Looking to isolate genomic DNA? Check out genomic DNA extraction by sample type

PCR purification kits

PCR clean-up is a routine but critical laboratory procedure. You need a PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.

Invitrogen DNA purification kits provide easier, faster, and safer methods for isolation of DNA fragments that yield superior results. Purified DNA fragments are ready for sequencing, additional PCR, transcription, mapping, cloning, and labeling.

Learn more about purifying your PCR product with PureLink PCR cleanup kits
See how the competitors stack up: Compare efficiency of PCR cleanup kits


Which PCR purification kit is right for you?

 Quick and efficient removal of by-productsConcentrates low-yield PCR
products
Simple, reliable, rapid method, 96-well formatRapid, scalable, magnetic bead
format
Product namePureLink PCR Purification KitPureLink PCR Micro Purification KitPureLink 96 PCR Purification KitChargeSwitch PCR Clean-Up Kit
FormatSilica columnSilica column96-well silica filter plateMagnetic beads
Prep time15 mins15 mins15 mins5 mins
Sample input volume50–100 µL50–100 µL50–100 µL25–50 µL
DNA fragment size100 bp to 15 kb100 bp to 15 kb100 bp to 15 kb90 bp to 40 kb
DNA binding capacityUp to 40 µgUp to 40 µgUp to 40 µg~25 µg per mg beads
Downstream applicationsSequencing, NGS, Nucleic Acid Labeling, PCR, CloningSequencing, Nucleic Acid Labeling, PCR, CloningSequencing, CloningSequencing, NGS, Microarray Analysis, PCR, Cloning
Automation compatibilityNoNoYesYes
Pack size50 preps
250 preps
50 preps
250 preps
4 plates100 preps
960 preps
Cat. No.K310001
K310002
K310050
K310250
K3100-96ACS12000
CS12000-10

 

Perform both PCR purification and gel extraction?

The Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit contains everything you need for both procedures.
 

Cell-free DNA isolation

Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA) and viruses. Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples.

Large volumes of biological fluids are sometimes required to obtain sufficient amounts of cfDNA for analysis. Invitrogen/Thermo Fisher Scientific offers scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types.

What is cell-free DNA?
 

Which cell-free DNA isolation kit is right for you?

 Fast isolation of viral nucleic acid
 
Easy-to-use, high sensitivity
 
Automated format, capable of processing large volumes of plasma
Product namePureLink Viral RNA/DNA Mini KitPureLink Pro 96 Viral RNA/DNA Purification KitMagMAX Cell-Free DNA Isolation Kit
Sample input volume500 µL200 µL0.5–10 mL
Compatible sample typesPlasma, serum, cerebrospinal fluidPlasma, serum, cerebrospinal fluidPlasma, serum, urine
Isolation technologySilica spin columnSilica microplateParamagnetic beads
Prep time15 mins35 mins24 samples in <1 hr
High-throughput compatibilityNoYesYes
Downstream applicationsCloning, qPCR, sequencing, genotypingCloning, qPCR, sequencing, genotypingCloning, qPCR, sequencing, genotyping
Prep size50 preps4 x 96 preps50 preps

Learn more about cell-free DNA isolation using MagMAX beads
 

Quick and easy DNA gel extraction

Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. High recovery of DNA fragments is critical to the success of the entire workflow.

Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15–30 minutes. The silica membrane technology captures DNA fragments without the need for pH adjustment.

With Invitrogen kits, DNA isolated from agarose gel is free of proteins, dye, and agarose; it is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.
 

Which DNA gel extraction kit is right for you?

 Environmentally
friendly
High DNA binding capacityYields highly concentrated DNA
Product namePureLink Quick Gel Extraction KitGeneJET Gel Extraction KitGeneJET Gel Extraction and DNA Cleanup Micro Kit
DNA binding capacity15 µg per column25 µg per column10 µg per column
Isolation technology & formatSilica spin/vacuum columnSilica spin/vacuum columnSilica spin/vacuum column
Prep time30 mins15 mins15 mins
DNA fragment size range40 bp to 10 kb100 bp to 20 kb25 bp to 20 kb
Elution volume50 µL20 to 50 µL6 to 10 µL
Automation compatibilityNoNoNo
Input sample typesAgarose gel slicesAgarose gel slicesAgarose gel slices, PCR and other enzymatic reaction mixtures

 

data.par.27463.image.457.259.1.dat-agarose-extraction-jpg

Figure 1. Amplification of DNA isolated using the PureLink Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR Safe DNA Gel Stain.

See how the competitors stack up: Comparison of DNA gel extraction kits
 

Green benefits of the PureLink Quick Gel Extraction Kit

  • Less hazardous reagents
  • Improved, lightweight, recyclable packaging
  • Decreased fuel consumption and greenhouse gas emissions for transport
  • Less waste disposal
     

Microbiome DNA purification Kit

The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil.

The kit uses proven PureLink spin column technology, combined with efficient triple-lysis approach and fast removal of inhibitors, to provide excellent yields of purified DNA from bacteria or fungi as well as host DNA.

The versatility of this microbiome DNA purification procedure makes it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

  Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples
Medical Science Review: The human microbiota: composition, functions, and therapeutic potential 
 

  • Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
  • Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
  • Streamlined protocols for a variety of biological samples
  • Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
     

One kit to isolate microbial and host DNA from diverse sample types

The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:

  • Stool
  • Urine
  • Saliva
  • Soil
  • Swabs (vaginal, buccal, skin, rectal, environmental)
  • Transport media
  • Growth media

PCR purification kits

PCR clean-up is a routine but critical laboratory procedure. You need a PCR clean-up method that efficiently removes short primers, unincorporated dNTPs, enzymes, short-failed PCR products, and salts from PCR reactions.

Invitrogen DNA purification kits provide easier, faster, and safer methods for isolation of DNA fragments that yield superior results. Purified DNA fragments are ready for sequencing, additional PCR, transcription, mapping, cloning, and labeling.

Learn more about purifying your PCR product with PureLink PCR cleanup kits
See how the competitors stack up: Compare efficiency of PCR cleanup kits


Which PCR purification kit is right for you?

 Quick and efficient removal of by-productsConcentrates low-yield PCR
products
Simple, reliable, rapid method, 96-well formatRapid, scalable, magnetic bead
format
Product namePureLink PCR Purification KitPureLink PCR Micro Purification KitPureLink 96 PCR Purification KitChargeSwitch PCR Clean-Up Kit
FormatSilica columnSilica column96-well silica filter plateMagnetic beads
Prep time15 mins15 mins15 mins5 mins
Sample input volume50–100 µL50–100 µL50–100 µL25–50 µL
DNA fragment size100 bp to 15 kb100 bp to 15 kb100 bp to 15 kb90 bp to 40 kb
DNA binding capacityUp to 40 µgUp to 40 µgUp to 40 µg~25 µg per mg beads
Downstream applicationsSequencing, NGS, Nucleic Acid Labeling, PCR, CloningSequencing, Nucleic Acid Labeling, PCR, CloningSequencing, CloningSequencing, NGS, Microarray Analysis, PCR, Cloning
Automation compatibilityNoNoYesYes
Pack size50 preps
250 preps
50 preps
250 preps
4 plates100 preps
960 preps
Cat. No.K310001
K310002
K310050
K310250
K3100-96ACS12000
CS12000-10

 

Perform both PCR purification and gel extraction?

The Invitrogen PureLink Quick Gel Extraction and PCR Purification Combo Kit contains everything you need for both procedures.
 

Cell-free DNA isolation

Biological fluid samples, such as plasma, serum, and urine, contain cell-free DNA (cfDNA) and viruses. Isolation of cfDNA and viral nucleic acid is challenging because of its low abundance in typical samples.

Large volumes of biological fluids are sometimes required to obtain sufficient amounts of cfDNA for analysis. Invitrogen/Thermo Fisher Scientific offers scalable products capable of handling these large volumes while efficiently recovering cfDNA from a broad range of sample types.

What is cell-free DNA?
 

Which cell-free DNA isolation kit is right for you?

 Fast isolation of viral nucleic acid
 
Easy-to-use, high sensitivity
 
Automated format, capable of processing large volumes of plasma
Product namePureLink Viral RNA/DNA Mini KitPureLink Pro 96 Viral RNA/DNA Purification KitMagMAX Cell-Free DNA Isolation Kit
Sample input volume500 µL200 µL0.5–10 mL
Compatible sample typesPlasma, serum, cerebrospinal fluidPlasma, serum, cerebrospinal fluidPlasma, serum, urine
Isolation technologySilica spin columnSilica microplateParamagnetic beads
Prep time15 mins35 mins24 samples in <1 hr
High-throughput compatibilityNoYesYes
Downstream applicationsCloning, qPCR, sequencing, genotypingCloning, qPCR, sequencing, genotypingCloning, qPCR, sequencing, genotyping
Prep size50 preps4 x 96 preps50 preps

Learn more about cell-free DNA isolation using MagMAX beads
 

Quick and easy DNA gel extraction

Routine isolation of DNA fragments from agarose gel is made easier with DNA gel extraction kits from Thermo Fisher Scientific. High recovery of DNA fragments is critical to the success of the entire workflow.

Invitrogen DNA gel extraction kits are designed to isolate DNA fragments from agarose gel in 15–30 minutes. The silica membrane technology captures DNA fragments without the need for pH adjustment.

With Invitrogen kits, DNA isolated from agarose gel is free of proteins, dye, and agarose; it is ready to use in a variety of applications, including DNA sequencing, PCR, in vitro transcription, restriction mapping, cloning, and labeling.
 

Which DNA gel extraction kit is right for you?

 Environmentally
friendly
High DNA binding capacityYields highly concentrated DNA
Product namePureLink Quick Gel Extraction KitGeneJET Gel Extraction KitGeneJET Gel Extraction and DNA Cleanup Micro Kit
DNA binding capacity15 µg per column25 µg per column10 µg per column
Isolation technology & formatSilica spin/vacuum columnSilica spin/vacuum columnSilica spin/vacuum column
Prep time30 mins15 mins15 mins
DNA fragment size range40 bp to 10 kb100 bp to 20 kb25 bp to 20 kb
Elution volume50 µL20 to 50 µL6 to 10 µL
Automation compatibilityNoNoNo
Input sample typesAgarose gel slicesAgarose gel slicesAgarose gel slices, PCR and other enzymatic reaction mixtures

 

data.par.27463.image.457.259.1.dat-agarose-extraction-jpg

Figure 1. Amplification of DNA isolated using the PureLink Quick Gel Extraction Kit. PCR amplicons varying in size from 100 bp to 5.4 kb were prepared using recombinant Taq DNA Polymerase. A portion of each PCR reaction was run on a 1% UltraPure Agarose gel (data not shown), and amplicon bands were excised and extracted using the PureLink Quick Gel Extraction Kit. Unpurified and gel-extracted PCR products were loaded onto a 1% agarose gel and visualized using SYBR Safe DNA Gel Stain.

See how the competitors stack up: Comparison of DNA gel extraction kits
 

Green benefits of the PureLink Quick Gel Extraction Kit

  • Less hazardous reagents
  • Improved, lightweight, recyclable packaging
  • Decreased fuel consumption and greenhouse gas emissions for transport
  • Less waste disposal
     

Microbiome DNA purification Kit

The Invitrogen PureLink Microbiome DNA Purification Kit enables rapid isolation of high-quality microbial and host DNA from a wide variety of sample types, including challenging samples such as stool and soil.

The kit uses proven PureLink spin column technology, combined with efficient triple-lysis approach and fast removal of inhibitors, to provide excellent yields of purified DNA from bacteria or fungi as well as host DNA.

The versatility of this microbiome DNA purification procedure makes it the ultimate kit for microbiome research projects as well as programs aimed at rapid detection of pathogenic bacteria in various samples.

  Application Note: Rapid 2-hour workflow for detection of select bacteria in stool samples
Medical Science Review: The human microbiota: composition, functions, and therapeutic potential 
 

  • Efficient lysis of all microorganisms (including durable species with thick and complex cell walls) by a combination of heat, chemical, and mechanical disruption with specialized beads
  • Elimination of inhibitory compounds by precipitation using a novel cleanup buffer
  • Streamlined protocols for a variety of biological samples
  • Recovery of highly pure DNA compatible with PCR, sequencing, and many other types of downstream analysis
     

One kit to isolate microbial and host DNA from diverse sample types

The PureLink Microbiome DNA Purification Kit is designed to eliminate the need to order “specialized” kits because it has been optimized for use with a wide range of biological samples. This versatile kit enables microbial (and host, where applicable) DNA purification from the following samples:

  • Stool
  • Urine
  • Saliva
  • Soil
  • Swabs (vaginal, buccal, skin, rectal, environmental)
  • Transport media
  • Growth media

Resources and support for PCR cleanup, DNA gel extraction, and cell-free DNA isolation

Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.

  • Protocol Videos—Videos to help you isolate nucleic acids using a variety of techniques.
  • Videos—View our library of DNA & RNA purification and analysis videos.
  • Application Notes—Explore our application notes from scientists sharing data for isolation products

For Research Use Only. Not for use in diagnostic procedures.