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pSilencer™ 2.1-U6 hygro
pSilencer™ 2.1-U6 hygro Vector Map
This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.
pSilencer™ 2.1-U6 hygro Annotated Promoter/Cloning Site Region
We recommend the following primers for sequencing:
T7 primer: 5'-TAATACGACTCACTATAGGG-3'
2.0rev primer: 5'-AGGCGATTAAGTTGGGTA-3'
pSilencer™ 2.1-U6 hygro Restriction Sites (Vector Size: 4885 bp)
Enzymes with single Restriction Sites
| Site | Enzyme |
|---|---|
| 1250 | aflIII |
| 2143 | AhdI |
| 1666 | AlwNI |
| 3994 | AsiSI |
| 4395 | AvaI |
| 4416 | AvrII |
| 461 | BamHI |
| 239 | BbeI |
| 2648 | BcgI |
| 2682 | BcgI' |
| 4365 | BclI |
| 2204 | BsaI |
| 797 | BseRI |
| 3123 | BsmI |
| 4039 | BspMI |
| 3069 | BstZ17I |
| 399 | HindIII |
| 235 | KasI |
| 3256 | NaeI |
| 236 | NarI |
| 3254 | NgoMIV |
| 1250 | PciI |
| 4329 | PshAI |
| 3182 | PsiI |
| 4023 | PstI |
| 3945 | RsrII |
| 3578 | SacII |
| 3060 | SalI |
| 1134 | SapI |
| 4648 | SexAI |
| 237 | SfoI |
| 3994 | SgfI |
| 4397 | SmaI |
| 4421 | StuI |
| 4395 | XmaI |
| 2742 | XmnI |
Non-Cutting Enzymes:
| Enzyme |
|---|
| AarI |
| Acc65I |
| AfeI |
| AflII |
| AgeI |
| AleI |
| AloI |
| AloI' |
| ApaI |
| AscI |
| BaeI |
| BaeI' |
| BanII |
| BbsI |
| BbvCI |
| BlpI |
| BmgBI |
| BmtI |
| Bpu10I |
| BsaBI |
| BsgI |
| BsiWI |
| BsrGI |
| BstBI |
| BstEII |
| BstXI |
| Bsu36I |
| BtrI |
| ClaI |
| EcoICRI |
| EcoNI |
| EcoRV |
| FalI |
| FalI' |
| FseI |
| FspAI |
| HpaI |
| KpnI |
| MfeI |
| MluI |
| MscI |
| NheI |
| NotI |
| NruI |
| PacI |
| PflMI |
| PmeI |
| PpuMI |
| PspOMI |
| PsrI |
| PsrI' |
| SacI |
| SanDI |
| SbfI |
| SfiI |
| SgrAI |
| SnaBI |
| SpeI |
| SrfI |
| SwaI |
| TaqII' |
| XbaI |
| XcmI |
| XhoI |