pSilencer™ 3.1-H1 hygro Vector Map

pSilencer™ 3.1-H1 hygro Vector Map

This map and the annotated sequence below portrays the circular negative control vector provided with the kit. It includes the negative control siRNA template (66 base pairs) between the BamH I and Hind III sites.

pSilencer™ 3.1-H1 hygro Annotated Promoter/Cloning Site Region

We recommend the following primers for sequencing:

M13F(-40): 5'-GTTTTCCCAGTCACGAC-3'

3.0rev: 5'-GAGTTAGCTCACTCATTAGGC-3'





pSilencer™ 3.1-H1 hygro Restriction Sites (Vector Size: 4552 bp)

Enzymes with single Restriction Sites

Site Enzyme
915 AflIII
1808 AhdI
1331 AlwNI
3661 AsiSI
4062 AvaI
4083 AvrII
495 BamHI
239 BbeI
2313 BcgI
2347 BcgI`
4032 BclI
4093 BseRI
2790 BsmI
3706 BspMI
2736 BstZ17I
4541 EcoO109I
556 HindIII
235 KasI
2923 NaeI
236 NarI
2921 NgoMIV
915 PciI
3996 PshAI
3690 PstI
3612 RsrII
3245 SacII
2727 SalI
799 SapI
4315 SexAI
237 SfoI
3661 SgfI
4064 SmaI
4088 StuI
4062 XmaI
2407 XmnI

Non-Cutting Enzymes:

Enzyme
AarI
Acc65I
AfeI
AflII
AgeI
AleI
AloI
AloI'
ApaI
AscI
BaeI
BaeI'
BanII
BbsI
BbvCI
BglII
BlpI
BmgBI
BmtI
Bpu10I
BsaBI
BsgI
BsiWI
BsrGI
BssHII
BstBI
BstEII
BstXI
Bsu36I
BtrI
ClaI
EcoICRI
EcoNI
EcoRV
FalI
FalI'
FseI
FspAI
HpaI
KpnI
MfeI
MluI
MscI
NheI
NotI
NruI
PacI
PflMI
PmeI
PpuMI
PspOMI
PsrI
PsrI'
SacI
SanDI
SbfI
SfiI
SgrAI
SnaBI
SpeI
SrfI
SwaI
TaqII'
XbaI
XcmI
XhoI