Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, molecular biology, and genetics to separate macromolecules, such as proteins or nucleic acids, based on their size and charge. PAGE involves the use of a polyacrylamide gel matrix, which serves as a medium through which the molecules can migrate when an electric field is applied. PAGE provides high resolution of DNA molecules between 10–3,000 bp long. Under optimal conditions, DNA molecules differing in single nucleotide can be resolved using polyacrylamide gels.

You can rely on our reagents, including UltraPure acrylamide, for pouring your own gels and hassle-free precast Invitrogen Novex polyacrylamide gels when running PAGE gels.

Learn more about acrylamide gels


Novex precast PAGE gels for DNA

We offer a wide variety of precast polyacrylamide Novex gels that can be used for standard or denaturing nucleic acid electrophoresis. To obtain the best result based on your application (PCR fragment separation, DNA retardation, etc.), it is important to choose the correct gel percentage, buffer system, and gel format. Figure 1 shows the migration pattern of DNA run on Novex polyacrylamide gels. Use the infographic to help select the optimal gel for your experiments.

Figure 1. DNA gel migration chart. The chart demonstrates patterns of DNA separation on Novex gels. Use the table below to select the optimal gel for your experiments. For optimal resolution, nucleic acid bands should migrate within the shaded areas.


PAGE gel for DNA selection guide

The size of Novex precast gels is 10 x 10 cm (gel size is 8 x 8 cm). We recommend using the XCell SureLock Mini-Cell Electrophoresis System to obtain optimal and consistent performance.

Novex TBE (Tris-Borate-EDTA) gels provide the highest resolution for DNA fragments 10–3,000 bp long. Use these gels for any DNA electrophoresis application, including analyzing restriction-enzyme digests, PCR products, southern analysis, and primer analysis. 

Novex TBE Gels, 4–20%
  • 15-well
  • 12-well
  • 10-well
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Novex TBE Gels, 6%
  • 15-well
  • 12-well
  • 10-well
  • 1-well
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Novex TBE Gels, 8%

  • 15-well
  • 12-well
  • 10-well
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Novex TBE Gels, 10%
  • 15-well
  • 12-well
  • 10-well
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Novex TBE Gels, 20%
  • 15-well
  • 12-well
  • 10-well
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Novex DNA Retardation Gels consist of 6% polyacrylamide prepared with 0.5X TBE as the gel buffer. They provide good resolution of 60–2,500 bp DNA fragments. 0.5X TBE buffer offers good fragment separation in electrophoresis, yet its ionic strength is low enough to promote DNA-protein interactions.

DNA Retardation Gels (6%), 1.0 mm
  • 15-well
  • 12-well
  • 10-well
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Novex TBE-Urea Gels are optimized for analysis and purification of single-stranded DNA or RNA 20–800 bases long. They are an excellent choice for synthetic oligonucleotide analysis and purification, RNase protection assays, in vitro transcription studies, and northern blot analysis.

6% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 6%
  • 15-well
  • 12-well
  • 10-well
Order here
10% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 10%
  • 15-well
  • 12-well
  • 10-well
Order here
15% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 15%
  • 15-well
  • 12-well
  • 10-well
Order here
For general DNA analysis

Novex TBE (Tris-Borate-EDTA) gels provide the highest resolution for DNA fragments 10–3,000 bp long. Use these gels for any DNA electrophoresis application, including analyzing restriction-enzyme digests, PCR products, southern analysis, and primer analysis. 

Novex TBE Gels, 4–20%
  • 15-well
  • 12-well
  • 10-well
Order here
Novex TBE Gels, 6%
  • 15-well
  • 12-well
  • 10-well
  • 1-well
Order here

Novex TBE Gels, 8%

  • 15-well
  • 12-well
  • 10-well
Order here
Novex TBE Gels, 10%
  • 15-well
  • 12-well
  • 10-well
Order here
Novex TBE Gels, 20%
  • 15-well
  • 12-well
  • 10-well
Order here
For DNA retardation and gel shift assays

Novex DNA Retardation Gels consist of 6% polyacrylamide prepared with 0.5X TBE as the gel buffer. They provide good resolution of 60–2,500 bp DNA fragments. 0.5X TBE buffer offers good fragment separation in electrophoresis, yet its ionic strength is low enough to promote DNA-protein interactions.

DNA Retardation Gels (6%), 1.0 mm
  • 15-well
  • 12-well
  • 10-well
Order here
For denaturing DNA or RNA analysis

Novex TBE-Urea Gels are optimized for analysis and purification of single-stranded DNA or RNA 20–800 bases long. They are an excellent choice for synthetic oligonucleotide analysis and purification, RNase protection assays, in vitro transcription studies, and northern blot analysis.

6% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 6%
  • 15-well
  • 12-well
  • 10-well
Order here
10% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 10%
  • 15-well
  • 12-well
  • 10-well
Order here
15% Novex TBE-Urea Gel cassettes and product pack
Novex TBE-Urea Gels, 15%
  • 15-well
  • 12-well
  • 10-well
Order here
Related products

For Research Use Only. Not for use in diagnostic procedures.

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Resources

Nucleic acid electrophoresis products
Explore Thermo Fisher Scientific’s suite of products for all your electrophoresis needs.

Nucleic acid electrophoresis education
Learn about gel electrophoresis basics, workflow, considerations, applications, and troubleshooting for separation and analysis of nucleic acids.

DNA and RNA protocols
Find easy-to-follow protocols for everyday experiments, including agarose protocols and mRNA protocols.

Support

Nucleic acid purification and analysis support center
Find tips, troubleshooting help, and resources for your nucleic acid purification and analysis applications.

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For Research Use Only. Not for use in diagnostic procedures.

Stylesheet for Classic Wide Template adjustments
Stylesheet for Classic Wide Template adjustments
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